ATPAF1 (NM_022745) Human Tagged ORF Clone

CAT#: RC209963

ATPAF1 (Myc-DDK-tagged)-Human ATP synthase mitochondrial F1 complex assembly factor 1 (ATPAF1), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY411584) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209963 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ATPAF1 protein (Cat# TP309963). The protein was produced from HEK293T cells transfected with ATPAF1 cDNA clone (Cat# RC209963) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATPAF1
• Synonyms: ATP11; ATP11p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209963 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGTGGTGGTGGCGGCTGCGGGTGGCGCGGGACCGGCGGTCCTGCAGGTGGCCGGTCTCTACC
  GGGGCCTGTGCGCGGTGCGCAGCCGCGCCCTGGGCCTGGGGCTCGTGTCACCCGCGCAGCTGCGCGTCTT
  CCCAGTGCGCCCCGGCTCGGGCCGGCCCGAGGGGGGCGCCGACGGCAGCGGGGTCGGGGCCGAGGCCGAG
  CTCCAGGCCAACCCTTTCTACGACCGCTACCGCGACAAGATCCAGCTGCTGCGCAGGTCAGACCCAGCTG
  CTTTTGAGTCCCGCCTGGAGAAACGCAGTGAATTTCGGAAGCAGCCAGTGGGGCATTCCAGGCAAGGTGA
  TTTTATCAAATGTGTGGAACAGAAGACAGATGCCTTGGGGAAACAGTCTGTGAACAGAGGATTCACTAAG
  GACAAGACTCTCAGTTCAATCTTTAACATTGAGATGGTAAAAGAAAAAACTGCAGAAGAAATAAAACAGA
  TTTGGCAGCAATATTTTGCAGCAAAAGATACAGTCTACGCAGTTATTCCTGCAGAAAAGTTTGATTTGAT
  CTGGAACCGGGCTCAGTCCTGTCCAACATTTCTATGTGCTCTGCCAAGAAGGGAAGGTTATGAGTTTTTT
  GTAGGACAATGGACAGGTACTGAACTCCACTTCACTGCACTTATAAATATTCAGACCCGAGGGGAAGCTG
  CAGCCAGCCAGCTGATTTTATATCACTATCCTGAACTTAAGGAAGAAAAGGGCATAGTGCTGATGACTGC
  AGAAATGGATTCCACATTTCTGAATGTTGCTGAGGCACAGTGCATCGCCAACCAAGTTCAGCTCTTCTAC
  GCTACTGATCGGAAAGAGACCTACGGGTTAGTGGAGACCTTTAACCTCAGACCAAATGAGTTCAAATATA
  TGTCTGTCATCGCTGAATTGGAGCAAAGCGGACTTGGAGCAGAACTGAAATGTGCCCAGAACCAAAATAA
  GACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209963 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAVVVAAAGGAGPAVLQVAGLYRGLCAVRSRALGLGLVSPAQLRVFPVRPGSGRPEGGADGSGVGAEAE
  LQANPFYDRYRDKIQLLRRSDPAAFESRLEKRSEFRKQPVGHSRQGDFIKCVEQKTDALGKQSVNRGFTK
  DKTLSSIFNIEMVKEKTAEEIKQIWQQYFAAKDTVYAVIPAEKFDLIWNRAQSCPTFLCALPRREGYEFF
  VGQWTGTELHFTALINIQTRGEAAASQLILYHYPELKEEKGIVLMTAEMDSTFLNVAEAQCIANQVQLFY
  ATDRKETYGLVETFNLRPNEFKYMSVIAELEQSGLGAELKCAQNQNKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022745
• ORF Size: 984 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022745.1, NM_022745.2, NM_022745.3, NP_073582.1, NP_073582.2
• RefSeq Size: 1849
• RefSeq ORF: 1056
• Locus ID: 64756
• MW: 36.4 kDa
• Gene Summary: This gene encodes an assembly factor for the F(1) component of the mitochondrial ATP synthase. This protein binds specifically to the F1 beta subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Aug 2011]