ATPAF1 (NM_022745) Human Tagged ORF Clone

CAT#: RG209963

  • TrueORF®

ATPAF1 (GFP-tagged) - Human ATP synthase mitochondrial F1 complex assembly factor 1 (ATPAF1), nuclear gene encoding mitochondrial protein, transcript variant 1


  "NM_022745" in other vectors (4)

Reconstitution Protocol

USD 460.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol ATPAF1
Synonyms ATP11; ATP11p
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG209963 representing NM_022745
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTGTGGTGGTGGCGGCTGCGGGTGGCGCGGGACCGGCGGTCCTGCAGGTGGCCGGTCTCTACC
GGGGCCTGTGCGCGGTGCGCAGCCGCGCCCTGGGCCTGGGGCTCGTGTCACCCGCGCAGCTGCGCGTCTT
CCCAGTGCGCCCCGGCTCGGGCCGGCCCGAGGGGGGCGCCGACGGCAGCGGGGTCGGGGCCGAGGCCGAG
CTCCAGGCCAACCCTTTCTACGACCGCTACCGCGACAAGATCCAGCTGCTGCGCAGGTCAGACCCAGCTG
CTTTTGAGTCCCGCCTGGAGAAACGCAGTGAATTTCGGAAGCAGCCAGTGGGGCATTCCAGGCAAGGTGA
TTTTATCAAATGTGTGGAACAGAAGACAGATGCCTTGGGGAAACAGTCTGTGAACAGAGGATTCACTAAG
GACAAGACTCTCAGTTCAATCTTTAACATTGAGATGGTAAAAGAAAAAACTGCAGAAGAAATAAAACAGA
TTTGGCAGCAATATTTTGCAGCAAAAGATACAGTCTACGCAGTTATTCCTGCAGAAAAGTTTGATTTGAT
CTGGAACCGGGCTCAGTCCTGTCCAACATTTCTATGTGCTCTGCCAAGAAGGGAAGGTTATGAGTTTTTT
GTAGGACAATGGACAGGTACTGAACTCCACTTCACTGCACTTATAAATATTCAGACCCGAGGGGAAGCTG
CAGCCAGCCAGCTGATTTTATATCACTATCCTGAACTTAAGGAAGAAAAGGGCATAGTGCTGATGACTGC
AGAAATGGATTCCACATTTCTGAATGTTGCTGAGGCACAGTGCATCGCCAACCAAGTTCAGCTCTTCTAC
GCTACTGATCGGAAAGAGACCTACGGGTTAGTGGAGACCTTTAACCTCAGACCAAATGAGTTCAAATATA
TGTCTGTCATCGCTGAATTGGAGCAAAGCGGACTTGGAGCAGAACTGAAATGTGCCCAGAACCAAAATAA
GACT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG209963 representing NM_022745
Red=Cloning site Green=Tags(s)

MAAVVVAAAGGAGPAVLQVAGLYRGLCAVRSRALGLGLVSPAQLRVFPVRPGSGRPEGGADGSGVGAEAE
LQANPFYDRYRDKIQLLRRSDPAAFESRLEKRSEFRKQPVGHSRQGDFIKCVEQKTDALGKQSVNRGFTK
DKTLSSIFNIEMVKEKTAEEIKQIWQQYFAAKDTVYAVIPAEKFDLIWNRAQSCPTFLCALPRREGYEFF
VGQWTGTELHFTALINIQTRGEAAASQLILYHYPELKEEKGIVLMTAEMDSTFLNVAEAQCIANQVQLFY
ATDRKETYGLVETFNLRPNEFKYMSVIAELEQSGLGAELKCAQNQNKT

TRTRRLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_022745
ORF Size 984 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_022745.3, NP_073582.2
RefSeq Size 4140
RefSeq ORF 1056
Locus ID 64756
Gene Summary This gene encodes an assembly factor for the F(1) component of the mitochondrial ATP synthase. This protein binds specifically to the F1 beta subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Aug 2011]

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