KCNJ5 (NM_000890) Human Tagged ORF Clone

CAT#: RC210184

KCNJ5 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 5 (KCNJ5)

Product Images

Western blot validation of overexpression lysate (Cat# LY424464) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210184 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified KCNJ5 protein (Cat# TP310184). The protein was produced from HEK293T cells transfected with KCNJ5 cDNA clone (Cat# RC210184) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNJ5
• Synonyms: CIR; GIRK4; KATP1; KIR3.4; LQT13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210184 representing NM_000890
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCGATTCTAGGAATGCCATGAACCAGGACATGGAGATTGGAGTCACTCCCTGGGACCCCAAGA
  AGATTCCAAAACAGGCCCGCGATTATGTCCCCATTGCCACAGACCGTACGCGCCTGCTGGCCGAGGGCAA
  GAAACCACGCCAGCGCTACATGGAGAAGAGCGGCAAGTGCAATGTGCACCACGGCAACGTCCAGGAGACC
  TACCGGTACCTGAGTGACCTCTTCACCACCCTGGTGGACCTCAAGTGGCGCTTCAACTTGCTCGTCTTCA
  CCATGGTTTACACTGTCACCTGGCTGTTCTTCGGCTTCATTTGGTGGCTCATTGCTTATATCCGGGGTGA
  CCTGGACCATGTTGGCGACCAAGAGTGGATTCCTTGTGTTGAAAACCTCAGTGGCTTCGTGTCCGCTTTC
  CTGTTCTCCATTGAGACCGAAACAACCATTGGGTATGGCTTCCGAGTCATCACAGAGAAGTGTCCAGAGG
  GGATTATACTCCTCTTGGTCCAGGCCATCCTGGGCTCCATCGTCAATGCCTTCATGGTGGGGTGCATGTT
  TGTCAAGATCAGCCAGCCCAAGAAGAGAGCGGAGACCCTCATGTTTTCCAACAACGCAGTCATCTCCATG
  CGGGACGAGAAGCTGTGCCTCATGTTCCGGGTGGGCGACCTCCGCAACTCCCACATCGTGGAGGCCTCCA
  TCCGGGCCAAGCTCATCAAGTCCCGGCAGACCAAAGAGGGGGAGTTCATCCCCCTGAACCAGACAGACAT
  CAACGTGGGCTTTGACACGGGCGACGACCGCCTCTTCCTGGTGTCTCCTCTGATCATCTCCCACGAGATC
  AACGAGAAGAGCCCTTTCTGGGAGATGTCTCAGGCTCAGCTGCATCAGGAAGAGTTTGAAGTTGTGGTCA
  TTCTAGAAGGGATGGTGGAAGCCACAGGCATGACCTGCCAAGCCCGGAGCTCCTACATGGATACAGAGGT
  GCTCTGGGGCCACCGATTCACACCAGTCCTCACCTTGGAAAAGGGCTTCTATGAGGTGGACTACAACACC
  TTCCATGATACCTATGAGACCAACACACCCAGCTGCTGTGCCAAGGAGCTGGCAGAAATGAAGAGGGAAG
  GCCGGCTCCTCCAGTACCTCCCCAGCCCCCCACTGCTGGGGGGCTGTGCTGAGGCAGGGCTGGATGCAGA
  GGCTGAGCAGAATGAAGAAGATGAGCCCAAGGGGCTGGGTGGGTCCAGGGAGGCCAGGGGCTCGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210184 representing NM_000890
  Red=Cloning site Green=Tags(s)
  
  MAGDSRNAMNQDMEIGVTPWDPKKIPKQARDYVPIATDRTRLLAEGKKPRQRYMEKSGKCNVHHGNVQET
  YRYLSDLFTTLVDLKWRFNLLVFTMVYTVTWLFFGFIWWLIAYIRGDLDHVGDQEWIPCVENLSGFVSAF
  LFSIETETTIGYGFRVITEKCPEGIILLLVQAILGSIVNAFMVGCMFVKISQPKKRAETLMFSNNAVISM
  RDEKLCLMFRVGDLRNSHIVEASIRAKLIKSRQTKEGEFIPLNQTDINVGFDTGDDRLFLVSPLIISHEI
  NEKSPFWEMSQAQLHQEEFEVVVILEGMVEATGMTCQARSSYMDTEVLWGHRFTPVLTLEKGFYEVDYNT
  FHDTYETNTPSCCAKELAEMKREGRLLQYLPSPPLLGGCAEAGLDAEAEQNEEDEPKGLGGSREARGSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000890
• ORF Size: 1257 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000890.2, NM_000890.3, NP_000881.3
• RefSeq Size: 2912 bp
• RefSeq ORF: 1260 bp
• Locus ID: 3762
• Cytogenetics: 11q24.3
• Domains: IRK
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 47.5 kDa
• Gene Summary: 'This gene encodes an integral membrane protein which belongs to one of seven subfamilies of inward-rectifier potassium channel proteins called potassium channel subfamily J. The encoded protein is a subunit of the potassium channel which is homotetrameric. It is controlled by G-proteins and has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Naturally occurring mutations in this gene are associated with aldosterone-producing adenomas. [provided by RefSeq, Aug 2017]'