KCNJ5 (NM_000890) Human Untagged Clone

CAT#: SC119590

KCNJ5 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 5 (KCNJ5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNJ5
• Synonyms: CIR; GIRK4; KATP1; KIR3.4; LQT13
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000890 edited
  GAATTCGGCACGAGGGAGAGGTTCCAGCTACAGCTCCTCCGTGGGGTCATGGCAGGGGCTGGGGAGTCCC
  CTCAAAAGCCCTGAGCCCCCCTGCACCGCCGCTAAGGGACACCCCAGAAGTTAGCATCAGTGGGACTCGG
  AAGCTCCGATCTCAACAACATCCCAGCTATGGCTGGCGATTCTAGGAATGCCATGAACCAGGACATGGAG
  ATTGGAGTCACTCCCTGGGACCCCAAGAAGATTCCAAAACAGGCCCGCGATTATGTCCCCATTGCCACAG
  ACCGTACGCGCCTGCTGGCCGAGGGCAAGAAGCCACGCCAGCGCTACATGGAGAAGAGCGGCAAGTGCAA
  TGTGCACCACGGCAACGTCCAGGAGACCTACCGGTACCTGAGTGACCTCTTCACCACCCTGGTGGACCTC
  AAGTGGCGCTTCAACTTGCTCGTCTTCACCATGGTTTACACTGTCACCTGGCTGTTCTTCGGCTTCATTT
  GGTGGCTCATTGCTTATATCCGGGGTGACCTGGACCATGTTGGCGACCAAGAGTGGATTCCTTGTGTTGA
  AAACCTCAGTGGCTTCGTGTCCGCTTTCCTGTTCTCCATTGAGACCGAAACAACCATTGGGTATGGCTTC
  CGAGTCATCACAGAGAAGTGTCCAGAGGGGATTATACTCCTCTTGGTCCAGGCCATCCTGGGCTCCATCG
  TCAATGCCTTCATGGTGGGGTGCATGTTTGTCAAGATCAGCCAGCCCAAGAAGAGAGCGGAGACCCTCAT
  GTTTTCCAACAACGCAGTCATCTCCATGCGGGACGAGAAGCTGTGCCTCATGTTCCGGGTGGGCGACCTC
  CGCAACTCCCACATCGTGGAGGCCTCCATCCGGGCCAAGCTCATCAAGTCCCGGCAGACCAAAGAGGGGG
  AGTTCATCCCCCTGAACCAGACAGACATCAACGTGGGCTTTGACACGGGCGACGACCGCCTCTTCCTGGT
  GTCTCCTCTGATCATCTCCCATGAGATCAACCAGAAGAGCCCTTTCTGGGAGATGTCTCAGGCTCAGCTG
  CATCAGGAAGAGTTTGAAGTTGTGGTCATTCTAGAAGGGATGGTGGAAGCCACAGGCATGACCTGCCAAG
  CCCGGAGCTCCTACATGGATACAGAGGTGCTCTGGGGCCACCGATTCACACCAGTCCTCACCTTGGAAAA
  GGGCTTCTATGAGGTGGACTACAACACCTTCCATGATACCTATGAGACCAACACACCCAGCTGCTGTGCC
  AAGGAGCTGGCAGAAATGAAGAGGGAAGGCCGGCTCCTCCAGTACCTCCCCAGCCCCCCACTGCTGGGGG
  GCTGTGCTGAGGCAGGGCTGGATGCAGAGGCTGAGCAGAATGAAGAAGATGAGCCCAAGGGGCTGGGTGG
  GTCCAGGGAGGCCAGGGGCTCGGTGTGAGGGGTGCAGCCTCCCTAAGACCTCCTGTCACTGGCTTCAGTG
  AACACAGACACTGCAGAGCCTGGGAGCAGGGGAGGGGAATAGTTGAGTGTGCTGTTTGGGGGCTCAGGAG
  CCATCAAGGCTGTGGGGAGGAACCATAAACCCAGCCCTCACAGCTCCCAGCACAGGGCCTCCCTGAGCCA
  GTGGCATCCTGCCTGGGCCCCCCATGGCAGTGCTGCCTCTTGTAGGTGCTGGCTAAGGGCCAGGCCAGGA
  TGAGTTTCCCCATGGTGAATGTTACCGGATGGCATCTGTTCTCTCCATGCCCTGGGTCACTTCCTTTTTT
  GGGTCTCACAGACCCCTCCAGGGGCTGACACCTAGAGAGAACCATCACCTTGTCCCTCATTCCTTCCACC
  CTGAGGCTTGCTGTGGACTCAGAGAGGAGACTTACCTGATGAGAGCTCAAACCTCTAGTCTGGGATGAGC
  TCACAGAGCCCTCATGAGTTAAGATCCATCCATATCACATAGGCAATTCCTTTTAAATCAGATACCAACG
  ACCCATTACTGAGAGGTACAGAGCGTACAGCCCTTGGTGTTCTCTGCTGTTATCTGCCAAATGTGTGTGT
  TTTTCCTCTGCTGTGTTCTGTGTGTCCAGTGTAGCCTATTGGAGAATCGGTCTCATTAGAGTGACTCTTA
  GAAGGTGGCCTGGGACTAGACAGCCCCTCTGAGCACATGCATAGTGGCATCAGCAGCTTCCTGGCCTACT
  CCCAATCACACTGTGCCCTGCACCTCCCACCAAGGGGGAACCCCAGCAGCTGGGGCTTTTCGAGTGCTTT
  TTGGCCCAAGAATCTCAGAGTGCTTCTAATCATCACAGTAGTTTTTGTGTTTTGTTTTGTTTTGTTTTGT
  TTTGTTTTGTTTTGTTTTGTTTTTTTCACAATCTCAGCCAGCCTGCGGTGTGACCTGGGGAGAAAGTTGA
  GTGTCAGGGGACATGATGAGCCAGTTGGCCCTGAAACAGGAATGGGGCAAAGAGAAAGGAGAGCCAGGAG
  CATTTAAAAATCAGCATCTGGGCCAGGCAAGATGGCTCACGCCTGTGATCCCAGCATTTTGGGAGGCCGA
  GGCGGGTGGATCATGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTACTGA
  AAATACAAAAAATTAGCCAGGCATGGTGGCAGGTGCCTGTAGTCCCAGCTACTCGGGAAGCTGAGGCAGA
  AGAATGGCATGAACCTGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATGCCACTGCACTCCAGCCTGGGC
  GACAGAGCGAGACTCCATCTCAAAAAAAAAAAAAAAAACTCGAC
• Restriction Sites: NotI-NotI
• ACCN: NM_000890
• Insert Size: 2770 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000890.3, NP_000881.3
• RefSeq Size: 2912 bp
• RefSeq ORF: 1260 bp
• Locus ID: 3762
• Cytogenetics: 11q24.3
• Domains: IRK
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'This gene encodes an integral membrane protein which belongs to one of seven subfamilies of inward-rectifier potassium channel proteins called potassium channel subfamily J. The encoded protein is a subunit of the potassium channel which is homotetrameric. It is controlled by G-proteins and has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Naturally occurring mutations in this gene are associated with aldosterone-producing adenomas. [provided by RefSeq, Aug 2017]'