LMX1B (NM_002316) Human Tagged ORF Clone
CAT#: RC210188
LMX1B (Myc-DDK-tagged)-Human LIM homeobox transcription factor 1, beta (LMX1B), transcript variant 1
"NM_002316" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | LMX1B |
Synonyms | LMX1.2; NPS1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210188 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGACGGCATCAAGATGGAGGAGCACGCCCTGCGCCCCGGGCCCGCCACTCTGGGGGTGCTGCTGG GCTCCGACTGCCCGCATCCCGCCGTCTGCGAGGGCTGCCAGCGGCCCATCTCCGACCGCTTCCTGATGCG AGTCAACGAGTCGTCCTGGCACGAGGAGTGTTTGCAGTGCGCGGCGTGTCAGCAAGCCCTCACCACCAGC TGCTACTTCCGGGATCGGAAACTGTACTGCAAACAAGACTACCAACAGCTCTTCGCGGCCAAGTGCAGCG GCTGCATGGAGAAGATCGCCCCCACCGAGTTCGTGATGCGGGCGCTGGAGTGCGTGTACCACCTGGGCTG CTTCTGCTGCTGCGTGTGTGAGCGGCAGCTACGCAAGGGCGACGAATTCGTGCTCAAGGAGGGCCAGCTG CTGTGCAAGGGTGACTACGAGAAGGAGAAGGACCTGCTCAGCTCCGTGAGCCCCGACGAGTCCGACTCCG TGAAGAGCGAGGATGAAGATGGGGACATGAAGCCGGCCAAGGGGCAGGGCAGTCAGAGCAAGGGCAGCGG GGATGACGGGAAGGACCCGCGGAGGCCCAAGCGACCCCGGACCATCCTCACCACGCAGCAGCGAAGAGCC TTCAAGGCCTCCTTCGAGGTCTCGTCCAAGCCTTGCCGAAAGGTCCGAGAGACACTGGCAGCTGAGACGG GCCTCAGTGTGCGCGTGGTCCAGGTCTGGTTTCAGAACCAAAGAGCAAAGATGAAGAAGCTGGCGCGGCG GCACCAGCAGCAGCAGGAGCAGCAGAACTCCCAGCGGCTGGGCCAGGAGGTCCTGTCCAGCCGCATGGAG GGCATGATGGCTTCCTACACGCCGCTGGCCCCACCACAGCAGCAGATCGTGGCCATGGAACAGAGCCCCT ACGGCAGCAGCGACCCCTTCCAGCAGGGCCTCACGCCGCCCCAAATGCCAGGGAACGACTCCATCTTCCA TGACATCGACAGCGATACCTCCTTAACCAGCCTCAGCGACTGCTTCCTCGGCTCCTCAGACGTGGGCTCC CTGCAGGCCCGCGTGGGGAACCCCATCGACCGGCTCTACTCCATGCAGAGTTCCTACTTCGCCTCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210188 protein sequence
Red=Cloning site Green=Tags(s) MLDGIKMEEHALRPGPATLGVLLGSDCPHPAVCEGCQRPISDRFLMRVNESSWHEECLQCAACQQALTTS CYFRDRKLYCKQDYQQLFAAKCSGCMEKIAPTEFVMRALECVYHLGCFCCCVCERQLRKGDEFVLKEGQL LCKGDYEKEKDLLSSVSPDESDSVKSEDEDGDMKPAKGQGSQSKGSGDDGKDPRRPKRPRTILTTQQRRA FKASFEVSSKPCRKVRETLAAETGLSVRVVQVWFQNQRAKMKKLARRHQQQQEQQNSQRLGQEVLSSRME GMMASYTPLAPPQQQIVAMEQSPYGSSDPFQQGLTPPQMPGNDSIFHDIDSDTSLTSLSDCFLGSSDVGS LQARVGNPIDRLYSMQSSYFAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002316 |
ORF Size | 1118 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002316.1, NM_002316.2, NP_002307.1 |
RefSeq Size | 5783 bp |
RefSeq ORF | 1188 bp |
Locus ID | 4010 |
Cytogenetics | 9q33.3 |
Protein Families | Transcription Factors |
MW | 41.6 kDa |
Gene Summary | 'This gene encodes a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains, 1 homeodomain, and a C-terminal glutamine-rich domain. It functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Mutations in this gene are associated with nail-patella syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC303179 | LMX1B (untagged)-Human LIM homeobox transcription factor 1, beta (LMX1B), transcript variant 1 |
USD 670.00 |
|
RG210188 | LMX1B (GFP-tagged) - Human LIM homeobox transcription factor 1, beta (LMX1B), transcript variant 1 |
USD 460.00 |
|
RC210188L1 | Lenti ORF clone of Human LIM homeobox transcription factor 1, beta (LMX1B), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC210188L2 | Lenti ORF clone of Human LIM homeobox transcription factor 1, beta (LMX1B), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC210188L3 | Lenti ORF clone of Human LIM homeobox transcription factor 1, beta (LMX1B), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC210188L4 | Lenti ORF clone of Human LIM homeobox transcription factor 1, beta (LMX1B), transcript variant 1, mGFP tagged |
USD 620.00 |
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