MMP16 (NM_005941) Human Tagged ORF Clone

CAT#: RC210199

MMP16 (Myc-DDK-tagged)-Human matrix metallopeptidase 16 (membrane-inserted) (MMP16)

Product Images

Western blot validation of overexpression lysate (Cat# LY416968) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210199 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MMP16
• Synonyms: C8orf57; MMP-X2; MT-MMP2; MT-MMP3; MT3-MMP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210199 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTTACTCACATTCAGCACTGGAAGACGGTTGGATTTCGTGCATCATTCGGGGGTGTTTTTCTTGC
  AAACCTTGCTTTGGATTTTATGTGCTACAGTCTGCGGAACGGAGCAGTATTTCAATGTGGAGGTTTGGTT
  ACAAAAGTACGGCTACCTTCCACCGACTGACCCCAGAATGTCAGTGCTGCGCTCTGCAGAGACCATGCAG
  TCTGCCCTAGCTGCCATGCAGCAGTTCTATGGCATTAACATGACAGGAAAAGTGGACAGAAACACAATTG
  ACTGGATGAAGAAGCCCCGATGCGGTGTACCTGACCAGACAAGAGGTAGCTCCAAATTTCATATTCGTCG
  AAAGCGATATGCATTGACAGGACAGAAATGGCAGCACAAGCACATCACTTACAGTATAAAGAACGTAACT
  CCAAAAGTAGGAGACCCTGAGACTCGTAAAGCTATTCGCCGTGCCTTTGATGTGTGGCAGAATGTAACTC
  CTCTGACATTTGAAGAAGTTCCCTACAGTGAATTAGAAAATGGCAAACGTGATGTGGATATAACCATTAT
  TTTTGCATCTGGTTTCCATGGGGACAGCTCTCCCTTTGATGGAGAGGGAGGATTTTTGGCACATGCCTAC
  TTCCCTGGACCAGGAATTGGAGGAGATACCCATTTTGACTCAGATGAGCCATGGACACTAGGAAATCCTA
  ATCATGATGGAAATGACTTATTTCTTGTAGCAGTCCATGAACTGGGACATGCTCTGGGATTGGAGCATTC
  CAATGACCCCACTGCCATCATGGCTCCATTTTACCAGTACATGGAAACAGACAACTTCAAACTACCTAAT
  GATGATTTACAGGGCATCCAGAAGATATATGGTCCACCTGACAAGATTCCTCCACCTACAAGACCTCTAC
  CGACAGTGCCCCCACACCGCTCTATTCCTCCGGCTGACCCAAGGAAAAATGACAGGCCAAAACCTCCTCG
  GCCTCCAACCGGCAGACCCTCCTATCCCGGAGCCAAACCCAACATCTGTGATGGGAACTTTAACACTCTA
  GCTATTCTTCGTCGTGAGATGTTTGTTTTCAAGGACCAGTGGTTTTGGCGAGTGAGAAACAACAGGGTGA
  TGGATGGATACCCAATGCAAATTACTTACTTCTGGCGGGGCTTGCCTCCTAGTATCGATGCAGTTTATGA
  AAATAGCGACGGGAATTTTGTGTTCTTTAAAGGTAACAAATATTGGGTGTTCAAGGATACAACTCTTCAA
  CCTGGTTACCCTCATGACTTGATAACCCTTGGAAGTGGAATTCCCCCTCATGGTATTGATTCAGCCATTT
  GGTGGGAGGACGTCGGGAAAACCTATTTCTTCAAGGGAGACAGATATTGGAGATATAGTGAAGAAATGAA
  AACAATGGACCCTGGCTATCCCAAGCCAATCACAGTCTGGAAAGGGATCCCTGAATCTCCTCAGGGAGCA
  TTTGTACACAAAGAAAATGGCTTTACGTATTTCTACAAAGGAAAGGAGTATTGGAAATTCAACAACCAGA
  TACTCAAGGTAGAACCTGGATATCCAAGATCCATCCTCAAGGATTTTATGGGCTGTGATGGACCAACAGA
  CAGAGTTAAAGAAGGACACAGCCCACCAGATGATGTAGACATTGTCATCAAACTGGACAACACAGCCAGC
  ACTGTGAAAGCCATAGCTATTGTCATTCCCTGCATCTTGGCCTTATGCCTCCTTGTATTGGTTTACACTG
  TGTTCCAGTTCAAGAGGAAAGGAACACCCCGCCACATACTGTACTGTAAACGCTCTATGCAAGAGTGGGT
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210199 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MILLTFSTGRRLDFVHHSGVFFLQTLLWILCATVCGTEQYFNVEVWLQKYGYLPPTDPRMSVLRSAETMQ
  SALAAMQQFYGINMTGKVDRNTIDWMKKPRCGVPDQTRGSSKFHIRRKRYALTGQKWQHKHITYSIKNVT
  PKVGDPETRKAIRRAFDVWQNVTPLTFEEVPYSELENGKRDVDITIIFASGFHGDSSPFDGEGGFLAHAY
  FPGPGIGGDTHFDSDEPWTLGNPNHDGNDLFLVAVHELGHALGLEHSNDPTAIMAPFYQYMETDNFKLPN
  DDLQGIQKIYGPPDKIPPPTRPLPTVPPHRSIPPADPRKNDRPKPPRPPTGRPSYPGAKPNICDGNFNTL
  AILRREMFVFKDQWFWRVRNNRVMDGYPMQITYFWRGLPPSIDAVYENSDGNFVFFKGNKYWVFKDTTLQ
  PGYPHDLITLGSGIPPHGIDSAIWWEDVGKTYFFKGDRYWRYSEEMKTMDPGYPKPITVWKGIPESPQGA
  FVHKENGFTYFYKGKEYWKFNNQILKVEPGYPRSILKDFMGCDGPTDRVKEGHSPPDDVDIVIKLDNTAS
  TVKAIAIVIPCILALCLLVLVYTVFQFKRKGTPRHILYCKRSMQEWV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005941
• ORF Size: 1821 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005941.1, NM_005941.2, NM_005941.3, NM_005941.4, NP_005932.2
• RefSeq Size: 6347 bp
• RefSeq ORF: 1824 bp
• Locus ID: 4325
• Cytogenetics: 8q21.3
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• MW: 69.5 kDa
• Gene Summary: 'Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq, Oct 2010]'