CA8 (NM_004056) Human Tagged ORF Clone
CAT#: RC210228L2
- LentiORF®
Lenti ORF clone of Human carbonic anhydrase VIII (CA8), mGFP tagged
"NM_004056" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | CA8 |
Synonyms | CA-RP; CA-VIII; CALS; CAMRQ3; CARP |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC210228).
|
Restriction Sites | SgfI-NotI Cloning Scheme for this gene |
ACCN | NM_004056 |
ORF Size | 870 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004056.4, NP_004047.3 |
RefSeq Size | 2278 bp |
RefSeq ORF | 873 bp |
Locus ID | 767 |
Cytogenetics | 8q12.1 |
Domains | carb_anhydrase |
Protein Families | Druggable Genome |
Protein Pathways | Nitrogen metabolism |
MW | 33 kDa |
Gene Summary | 'The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in this gene are associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3). Polymorphisms in this gene are associated with osteoporosis, and overexpression of this gene in osteosarcoma cells suggests an oncogenic role. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC117603 | CA8 (untagged)-Human carbonic anhydrase VIII (CA8) |
USD 310.00 |
|
RC210228 | CA8 (Myc-DDK-tagged)-Human carbonic anhydrase VIII (CA8) |
USD 420.00 |
|
RG210228 | CA8 (GFP-tagged) - Human carbonic anhydrase VIII (CA8) |
USD 460.00 |
|
RC210228L1 | Lenti ORF clone of Human carbonic anhydrase VIII (CA8), Myc-DDK-tagged |
USD 768.00 |
|
RC210228L3 | Lenti ORF clone of Human carbonic anhydrase VIII (CA8), Myc-DDK-tagged |
USD 620.00 |
|
RC210228L4 | Lenti ORF clone of Human carbonic anhydrase VIII (CA8), mGFP tagged |
USD 620.00 |
{0} Product Review(s)
Be the first one to submit a review