CA8 (NM_004056) Human Untagged Clone

CAT#: SC117603

CA8 (untagged)-Human carbonic anhydrase VIII (CA8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CA8
• Synonyms: CA-RP; CA-VIII; CALS; CAMRQ3; CARP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117603 sequence for NM_004056 edited (data generated by NextGen Sequencing)
  ATGGCGGACCTGAGCTTCATCGAAGATACCGTCGCCTTCCCCGAGAAGGAAGAGGATGAG
  GAGGAAGAAGAGGAGGGTGTGGAGTGGGGCTACGAGGAAGGTGTTGAGTGGGGTCTGGTG
  TTTCCTGATGCTAATGGGGAATACCAGTCTCCTATTAACCTAAACTCAAGAGAGGCTAGG
  TATGACCCCTCGCTGTTGGATGTCCGCCTCTCCCCAAATTATGTGGTGTGCCGAGACTGT
  GAAGTCACCAATGATGGACATACCATTCAGGTTATCCTGAAGTCAAAATCAGTTCTTTCG
  GGAGGACCATTGCCTCAAGGGCATGAGTTTGAACTGTACGAAGTGAGATTTCACTGGGGA
  AGAGAAAACCAGCGTGGTTCTGAGCACACGGTTAATTTCAAAGCTTTTCCCATGGAGCTC
  CATCTGATCCACTGGAACTCCACTCTGTTTGGCAGCATTGATGAGGCTGTGGGGAAGCCG
  CACGGAATCGCCATCATTGCTCTGTTTGTTCAGATAGGAAAGGAACATGTTGGCTTGAAG
  GCTGTGACTGAAATCCTCCAAGATATTCAGTATAAGGGGAAGTCCAAAACAATACCTTGC
  TTTAATCCTAACACTTTATTACCAGACCCTCTGCTGCGGGATTACTGGGTGTATGAAGGC
  TCTCTCACCATCCCACCTTGCAGTGAAGGTGTCACCTGGATATTATTCCGATACCCTTTA
  ACTATATCCCAGCTACAGATAGAAGAATTTCGAAGGCTGAGGACACATGTTAAGGGGGCA
  GAACTTGTGGAAGGCTGTGATGGGATTTTGGGAGACAACTTTCGGCCCACTCAGCCTCTT
  AGTGACAGAGTCATTAGAGCTGCATTTCAGTAG
  
  Clone variation with respect to NM_004056.4
  327 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004056 unedited
  ATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGTCGCTCTGCTGGG
  GCCTCCGGACGCGCTTCCCAGCGGGTCTCTGGAACACTCGGTCCGAACGCACGCCTGCTT
  GCACTCACACTGCGGTTCACACCCGCAGGCGCTCTCGCACCCACACTGCCGCTCACGCGC
  GCTCACACTCCCCCACGCGCGCTCCGCTCCGGCTCCAGCCCCGCGCCCCGCGAAGGCGCA
  GGCACTGCTGCCGAGAGCGCCGAGGGGCCCCGCGGCCTTCCCATGGCGGACCTGAGCTTC
  ATCGAAGATACCGTCGCCTTCCCCGAGAAGGAAGAGGATGAGGAGGAAGAAGAGGAGGGT
  GTGGAGTGGGGCTACGAGGAAGGTGTTGAGTGGGGTCTGGTGTTTCCTGATGCTAATGGG
  GAATACCAGTCTCCTATTAACCTAAACTCAAGAGAGGCTAGGTATGACCCCTCGCTGTTG
  GATGTCCGCCTCTCCCCAAATTATGTGGTGTGCCGAGACTGTGAAGTCACCAATGATGGA
  CATACCATTCAGGTTATCCTGAAGTCAAAATCAGTTCTTTCGGGAGGACCATTGCCTCAA
  GGGCATGAGTTTGAACTGTACGAAGTGAGATTTCACTGGGGAAGAGAAAACCAGCGTGGT
  TCTGAGCACACGGTTAATTTCAAAGCTTTTCCCATGGAGCTCCATCTGATCCACTGGAAC
  TCCACTCTGTTTGGCAGCATTGATGAGGCTGTGGGGAAGCCGCACGGAATCGCCATCATT
  GCTCTGTTTGTTCAGATAGGAAAGAACATGTTNNGCTGAGGCTGTGACTGANATCCTCCA
  AGAATTCAGTATAAGGGGGAAGTCCAAACAATACCTTGCTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004056 unedited
  AGCTATGGACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTCATT
  TCAGTCTTTTACTCAAAAAATATACAGTAAATAAATTCAAATTGCTTCAATATACAGACA
  TAAACACACATACACACACACCTGTGAAGGGTGAAGTCTGAACAACTAGATGTTATCCTG
  AAAGTCATTTAATTACTATTTGTTATTGTATTTGTTTATTTTGTGTATTTCAGGGAAAGA
  TGAAAGAAGATTGCAAGAAGCATCAGAAGTCTAGTTCAAGAACAATCCCTGACTGTTCAG
  CACCAACCCGGTCAGGAGTGGAAAGGCTGGTACCTGTGCAGTGTTTTGTTCCAGGAATGC
  CATATGAATGGCCTCTCATTTATGGAAGGCTTCAAATCTAGTCTTTTGATGTTAATTACA
  AATAAGGCTGCACGTGTGCAGCCAATGTTTGGCATGTGAGTGGTAACAGTGCTAGATGTA
  AATATTTTTGTCACAGTTTTGTAACCATGCTTCACCATTTCCTCATTGTTGGACACTAGG
  AACTGTTATACATAAGGGAAGTCCAGGACCTCTCTCCGCTTTCAGAGGCACTGCCTGGTG
  CTGACCCCCATGTGATCCCAGGGCTACCCAACAGATCTCAACCCCTTAGCAAGCCTCAAC
  TTTTCCACACATTGCCATAACCCTAGTCCTTTCTCTTGCTGCCCAAGAAACATGATGAAC
  TTTCTTTGCTTGAATTTAGGGATTTCTGGTGTGATTTTCATTTGNATTAAAGGCTCCCAT
  TATTACCGCCTTTACTCAACAAATCTAANAACTCTGCACATATACTTATTACACCTGTCT
  TCTATTTCTATGACTTAGATATCTTGCATCACAAAATCCCTCCAACTTGGGCACTATGTT
  TTTGAAGCCATTCATTTTACAATCTTACATCCATTACCCTATTCTTATTGTGTGCTTTGA
  G
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004056
• Insert Size: 2870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004056.4, NP_004047.3
• RefSeq Size: 2278 bp
• RefSeq ORF: 873 bp
• Locus ID: 767
• Cytogenetics: 8q12.1
• Domains: carb_anhydrase
• Protein Families: Druggable Genome
• Protein Pathways: Nitrogen metabolism
• Gene Summary: 'The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in this gene are associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3). Polymorphisms in this gene are associated with osteoporosis, and overexpression of this gene in osteosarcoma cells suggests an oncogenic role. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]'
  Transcript Variant: This variant (1) encodes the longest isoform (a). Both variants 1 and 2 encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.