GRK2 (NM_001619) Human Tagged ORF Clone

CAT#: RC210327L4

Lenti ORF clone of Human adrenergic, beta, receptor kinase 1 (ADRBK1), mGFP tagged

Product Images

Double digestion of RC210327L4 using SgfI and RsrII

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: GRK2
• Synonyms: ADRBK1; BARK1; BETA-ARK1
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC210327).
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene
• ACCN: NM_001619
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001619.2
• RefSeq Size: 3603 bp
• RefSeq ORF: 2070 bp
• Locus ID: 156
• UniProt ID: P25098, A0A0S2Z392
• Cytogenetics: 11q13.2
• Domains: RGS, pkinase, S_TK_X, TyrKc, PH, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• MW: 79.4 kDa
• Gene Summary: 'This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017]'