TBC1D24 (NM_020705) Human Tagged ORF Clone

CAT#: RC210346

TBC1D24 (Myc-DDK-tagged)-Human TBC1 domain family, member 24 (TBC1D24), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY412201) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210346 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TBC1D24 protein (Cat# TP310346). The protein was produced from HEK293T cells transfected with TBC1D24 cDNA clone (Cat# RC210346) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TBC1D24
• Synonyms: DFNA65; DFNB86; DOORS; EIEE16; FIME; TLDC6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210346 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCTCCAGGATACAACTGCTTCGTGGACAAAGACAAGATGGACGCTGCCATCCAGGACCTGGGGC
  CCAAGGAGCTGAGCTGCACTGAACTGCAGGAACTGAAGCAGCTGGCGCGCCAGGGCTACTGGGCCCAAAG
  CCACGCCCTGCGGGGAAAGGTGTACCAGCGCCTGATCCGGGACATTCCCTGCCGCACGGTCACGCCTGAC
  GCCAGCGTGTACAGCGACATCGTGGGCAAGATCGTGGGCAAGCACAGCAGCAGCTGCCTGCCGCTGCCCG
  AGTTCGTGGACAACACGCAGGTGCCCAGCTACTGCCTGAATGCACGCGGCGAGGGGGCCGTGCGCAAGAT
  CCTCCTGTGCCTGGCCAACCAGTTCCCCGACATCTCCTTCTGCCCCGCCCTGCCGGCCGTGGTGGCCCTG
  CTGCTGCACTACAGCATCGACGAGGCCGAGTGCTTCGAGAAGGCCTGCCGCATCCTGGCCTGCAATGACC
  CCGGCAGGAGGCTGATCGACCAGAGCTTCCTGGCCTTTGAGTCGTCCTGCATGACGTTTGGGGACCTGGT
  GAACAAGTACTGCCAGGCGGCCCACAAGCTGATGGTGGCCGTGTCGGAGGATGTCCTGCAGGTCTATGCG
  GACTGGCAGCGCTGGCTGTTTGGGGAGCTGCCCCTCTGCTACTTCGCCCGGGTCTTTGACGTCTTCCTGG
  TGGAGGGCTACAAGGTGCTGTACCGCGTGGCGCTGGCCATCCTCAAGTTCTTCCACAAGGTGAGGGCCGG
  GCAGCCGCTGGAGTCGGACAGCGTGAAGCAGGACATCCGCACGTTCGTCAGAGACATCGCGAAGACGGTG
  TCCCCTGAGAAGCTGCTGGAGAAAGCGTTCGCCATCCGCCTCTTCTCCCGCAAGGAGATCCAGCTCCTGC
  AGATGGCCAATGAGAAAGCCCTGAAGCAGAAGGGCATCACCGTGAAGCAGAAGAGGCAGTTTGTACACTT
  GGCCGTCCATGCAGAGAACTTCCGCTCGGAGATCGTCAGCGTGAGGGAGATGAGAGACATCTGGTCCTGG
  GTCCCCGAGCGCTTTGCCCTGTGCCAGCCCCTTCTGCTGTTCTCCTCCCTGCAGCACGGGTACAGCCTGG
  CCAGGTTCTACTTCCAGTGTGAAGGACATGAGCCTACCCTCTTGCTCATCAAGACCACGCAGAAGGAGGT
  GTGTGGTGCTTACCTGTCCACAGACTGGAGTGAGAGAAATAAGTTTGGAGGCAAACTGGGCTTCTTTGGG
  ACCGGAGAATGCTTTGTGTTTAGGCTGCAGCCTGAGGTGCAGCGCTACGAGTGGGTGGTGATCAAGCACC
  CCGAGCTGACCAAGCCCCCACCCTTGATGGCTGCCGAGCCCACCGCCCCACTCAGCCACTCCGCCTCCTC
  AGACCCCGCTGACCGCCTCTCGCCCTTCCTGGCCGCTCGCCACTTCAACCTGCCCTCCAAGACCGAGTCC
  ATGTTCATGGCGGGGGGCAGCGACTGCCTCATCGTCGGGGGAGGAGGCGGCCAGGCGCTCTACATCGATG
  GGGACCTGAACCGGGGCCGCACAAGCCACTGCGACACCTTCAACAACCAGCCCCTCTGCTCCGAGAACTT
  CCTCATTGCTGCCGTGGAGGCCTGGGGCTTCCAGGACCCTGACACCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210346 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDSPGYNCFVDKDKMDAAIQDLGPKELSCTELQELKQLARQGYWAQSHALRGKVYQRLIRDIPCRTVTPD
  ASVYSDIVGKIVGKHSSSCLPLPEFVDNTQVPSYCLNARGEGAVRKILLCLANQFPDISFCPALPAVVAL
  LLHYSIDEAECFEKACRILACNDPGRRLIDQSFLAFESSCMTFGDLVNKYCQAAHKLMVAVSEDVLQVYA
  DWQRWLFGELPLCYFARVFDVFLVEGYKVLYRVALAILKFFHKVRAGQPLESDSVKQDIRTFVRDIAKTV
  SPEKLLEKAFAIRLFSRKEIQLLQMANEKALKQKGITVKQKRQFVHLAVHAENFRSEIVSVREMRDIWSW
  VPERFALCQPLLLFSSLQHGYSLARFYFQCEGHEPTLLLIKTTQKEVCGAYLSTDWSERNKFGGKLGFFG
  TGECFVFRLQPEVQRYEWVVIKHPELTKPPPLMAAEPTAPLSHSASSDPADRLSPFLAARHFNLPSKTES
  MFMAGGSDCLIVGGGGGQALYIDGDLNRGRTSHCDTFNNQPLCSENFLIAAVEAWGFQDPDTQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020705
• ORF Size: 1659 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020705.1, NM_020705.2, NP_065756.1
• RefSeq Size: 6589 bp
• RefSeq ORF: 1662 bp
• Locus ID: 57465
• MW: 62.3 kDa
• Gene Summary: This gene encodes a protein with a conserved domain, referred to as the TBC domain, characteristic of proteins which interact with GTPases. TBC domain proteins may serve as GTPase-activating proteins for a particular group of GTPases, the Rab (Ras-related proteins in brain) small GTPases which are involved in the regulation of membrane trafficking. Mutations in this gene are associated with familial infantile myoclonic epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]