Proteinase Activated Receptor 4 (F2RL3) (NM_003950) Human Tagged ORF Clone

CAT#: RC210404

F2RL3 (Myc-DDK-tagged)-Human coagulation factor II (thrombin) receptor-like 3 (F2RL3)

Product Images

Western blot validation of overexpression lysate (Cat# LY401297) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210404 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified F2RL3 protein (Cat# TP310404). The protein was produced from HEK293T cells transfected with F2RL3 cDNA clone (Cat# RC210404) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: F2RL3
• Synonyms: PAR4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210404 representing NM_003950
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGGGCGACTGCTCCTGTGGCCCCTGGTGCTGGGGTTCAGCCTGTCTGGCGGCACCCAGACCCCCA
  GCGTCTACGACGAGAGCGGGAGCACCGGAGGTGGTGATGACAGCACGCCCTCAATCCTGCCTGCCCCCCG
  CGGCTACCCAGGCCAAGTCTGTGCCAATGACAGTGACACCCTGGAGCTCCCGGACAGCTCACGGGCACTG
  CTTCTGGGCTGGGTGCCCACCAGGCTGGTGCCCGCCCTCTATGGGCTGGTCCTGGTGGTGGGGCTGCCGG
  CCAATGGGCTGGCGCTGTGGGTGCTGGCCACGCAGGCACCTCGGCTGCCCTCCACCATGCTGCTGATGAA
  CCTCGCGGCTGCTGACCTCCTGCTGGCCCTGGCGCTGCCCCCGCGGATCGCCTACCACCTGCGTGGCCAG
  CGCTGGCCCTTCGGGGAGGCCGCCTGCCGCCTGGCCACGGCCGCACTCTATGGTCACATGTATGGCTCAG
  TGCTGCTGCTGGCCGCCGTCAGCCTGGATCGCTACCTGGCCCTGGTGCACCCGCTGCGGGCCCGCGCCCT
  GCGTGGCCGGCGCCTGGCCCTTGGACTCTGCATGGCTGCTTGGCTCATGGCGGCCGCCCTGGCACTGCCC
  CTGACACTGCAGCGGCAGACCTTCCGGCTGGCGCGCTCCGATCGCGTGCTCTGCCATGACGCGCTGCCCC
  TGGACGCACAGGCCTCCCACTGGCAACCGGCCTTCACCTGCCTGGCGCTGTTGGGCTGTTTCCTGCCCCT
  GCTGGCCATGCTGCTGTGCTACGGGGCCACCCTGCACACGCTGGCGGCCAGCGGCCGGCGCTACGGCCAC
  GCGCTGAGGCTGACCGCAGTGGTGCTGGCCTCCGCCGTGGCCTTCTTCGTGCCCAGCAACCTGCTGCTGC
  TGCTGCATTACTCGGACCCGAGCCCCAGCGCCTGGGGCAACCTCTATGGTGCCTACGTGCCCAGCCTGGC
  GCTGAGCACCCTCAACAGCTGCGTGGATCCCTTCATCTACTACTACGTGTCGGCCGAGTTCAGGGACAAG
  GTGCGGGCAGGGCTCTTCCAACGGTCGCCGGGGGACACCGTGGCCTCCAAGGCCTCTGCGGAAGGGGGCA
  GCCGGGGCATGGGCACCCACTCCTCTTTGCTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210404 representing NM_003950
  Red=Cloning site Green=Tags(s)
  
  MWGRLLLWPLVLGFSLSGGTQTPSVYDESGSTGGGDDSTPSILPAPRGYPGQVCANDSDTLELPDSSRAL
  LLGWVPTRLVPALYGLVLVVGLPANGLALWVLATQAPRLPSTMLLMNLAAADLLLALALPPRIAYHLRGQ
  RWPFGEAACRLATAALYGHMYGSVLLLAAVSLDRYLALVHPLRARALRGRRLALGLCMAAWLMAAALALP
  LTLQRQTFRLARSDRVLCHDALPLDAQASHWQPAFTCLALLGCFLPLLAMLLCYGATLHTLAASGRRYGH
  ALRLTAVVLASAVAFFVPSNLLLLLHYSDPSPSAWGNLYGAYVPSLALSTLNSCVDPFIYYYVSAEFRDK
  VRAGLFQRSPGDTVASKASAEGGSRGMGTHSSLLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003950
• ORF Size: 1155 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003950.1, NM_003950.2, NM_003950.3, NP_003941.1, NP_003941.2
• RefSeq Size: 2731 bp
• RefSeq ORF: 1158 bp
• Locus ID: 9002
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 41.13 kDa
• Gene Summary: This gene encodes a member of the protease-activated receptor subfamily, part of the G-protein coupled receptor 1 family of proteins. The encoded receptor is proteolytically processed to reveal an extracellular N-terminal tethered ligand that binds to and activates the receptor. This receptor plays a role in blood coagulation, inflammation and response to pain. Hypomethylation at this gene may be associated with lung cancer in human patients. [provided by RefSeq, Sep 2016]