AVPR1A (NM_000706) Human Tagged ORF Clone

CAT#: RC210436

AVPR1A (Myc-DDK-tagged)-Human arginine vasopressin receptor 1A (AVPR1A)

Product Images

Western blot validation of overexpression lysate (Cat# LY424557) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210436 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AVPR1A
• Synonyms: AVPR1; AVPR V1a; V1aR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210436 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTCTCTCCGCCGGTCCCGACGCGGGGCCCTCGGGCAACTCCAGCCCATGGTGGCCTCTGGCCACCG
  GCGCTGGCAACACAAGCCGGGAGGCCGAAGCCCTCGGGGAGGGCAACGGCCCACCGAGGGACGTGCGCAA
  CGAGGAGCTGGCCAAACTGGAGATCGCCGTGCTGGCGGTGACTTTCGCGGTGGCCGTGCTGGGCAACAGC
  AGCGTACTGCTGGCTCTGCACCGGACGCCGCGCAAGACGTCCCGCATGCACCTCTTCATCCGACACCTCA
  GCCTGGCCGACCTGGCCGTGGCATTCTTCCAGGTGCTGCCGCAAATGTGCTGGGACATCACCTACCGCTT
  CCGCGGCCCCGACTGGCTGTGCCGCGTGGTGAAGCACCTGCAGGTGTTCGGCATGTTTGCGTCGGCCTAC
  ATGCTGGTAGTCATGACAGCCGACCGCTACATCGCGGTGTGCCACCCGCTCAAGACTCTGCAACAGCCCG
  CGCGCCGCTCGCGCCTCATGATCGCGGCCGCCTGGGTGCTGAGCTTCGTGCTGAGCACGCCGCAGTACTT
  CGTCTTCTCCATGATCGAGGTGAACAATGTCACCAAGGCCCGCGACTGCTGGGCCACCTTCATCCAGCCC
  TGGGGTTCTCGTGCCTACGTGACCTGGATGACGGGCGGCATCTTTGTGGCGCCCGTGGTCATCTTGGGTA
  CCTGCTACGGCTTCATCTGCTACAACATCTGGTGCAACGTCCGCGGGAAGACGGCGTCGCGCCAGAGCAA
  GGGTGCAGAGCAAGCGGGTGTGGCCTTCCAAAAGGGGTTCCTGCTCGCACCCTGTGTCAGCAGCGTGAAG
  TCCATTTCCCGGGCCAAGATCCGCACGGTGAAGATGACTTTTGTGATCGTGACGGCTTACATCGTCTGCT
  GGGCGCCTTTCTTCATCATCCAGATGTGGTCTGTCTGGGATCCCATGTCCGTCTGGACCGAATCGGAAAA
  CCCTACCATCACCATCACTGCATTACTGGGTTCCTTGAATAGCTGCTGTAATCCCTGGATATACATGTTT
  TTTAGTGGCCATCTCCTTCAAGACTGTGTTCAAAGCTTCCCATGCTGCCAAAACATGAAGGAAAAATTCA
  ACAAAGAAGATACTGACAGTATGAGCAGAAGACAGACTTTTTATTCTAACAATCGAAGCCCAACAAACAG
  TACGGGTATGTGGAAGGACTCGCCTAAATCTTCCAAGTCCATCAAATTCATTCCTGTTTCAACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210436 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRLSAGPDAGPSGNSSPWWPLATGAGNTSREAEALGEGNGPPRDVRNEELAKLEIAVLAVTFAVAVLGNS
  SVLLALHRTPRKTSRMHLFIRHLSLADLAVAFFQVLPQMCWDITYRFRGPDWLCRVVKHLQVFGMFASAY
  MLVVMTADRYIAVCHPLKTLQQPARRSRLMIAAAWVLSFVLSTPQYFVFSMIEVNNVTKARDCWATFIQP
  WGSRAYVTWMTGGIFVAPVVILGTCYGFICYNIWCNVRGKTASRQSKGAEQAGVAFQKGFLLAPCVSSVK
  SISRAKIRTVKMTFVIVTAYIVCWAPFFIIQMWSVWDPMSVWTESENPTITITALLGSLNSCCNPWIYMF
  FSGHLLQDCVQSFPCCQNMKEKFNKEDTDSMSRRQTFYSNNRSPTNSTGMWKDSPKSSKSIKFIPVST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000706
• ORF Size: 1254 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000706.1, NM_000706.2, NM_000706.3, NM_000706.4, NP_000697.1
• RefSeq Size: 7844 bp
• RefSeq ORF: 1257 bp
• Locus ID: 552
• Cytogenetics: 12q14.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Vascular smooth muscle contraction
• MW: 46.8 kDa
• Gene Summary: 'The protein encoded by this gene acts as receptor for arginine vasopressin. This receptor belongs to the subfamily of G-protein coupled receptors which includes AVPR1B, V2R and OXT receptors. Its activity is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. The receptor mediates cell contraction and proliferation, platelet aggregation, release of coagulation factor and glycogenolysis. [provided by RefSeq, Jul 2008]'