PVRIG (NM_024070) Human Tagged ORF Clone

CAT#: RC210470

PVRIG (Myc-DDK-tagged)-Human poliovirus receptor related immunoglobulin domain containing (PVRIG)

Product Images

Western blot validation of overexpression lysate (Cat# LY411386) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210470 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PVRIG
• Synonyms: C7orf15; CD112R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210470 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAACAGAGGCACAGGTGCCGGCCCTGCAGCCCCCAGAACCTGGACTGGAGGGGGCCATGGGGCACC
  GGACCCTGGTCCTGCCCTGGGTGCTGCTGACCTTGTGTGTCACTGCGGGGACCCCGGAGGTGTGGGTTCA
  AGTTCGGATGGAGGCCACCGAGCTCTCGTCCTTCACCATCCGTTGTGGGTTCCTGGGGTCTGGCTCCATC
  TCCCTGGTGACTGTGAGCTGGGGGGGCCCCAACGGTGCTGGGGGGACCACGCTGGCTGTGTTGCACCCAG
  AACGTGGCATCCGGCAATGGGCCCCTGCTCGCCAGGCCCGCTGGGAAACCCAGAGCAGCATCTCTCTCAT
  CCTGGAAGGCTCTGGGGCCAGCAGCCCCTGCGCCAACACCACCTTCTGCTGCAAGTTTGCGTCCTTCCCT
  GAGGGCTCCTGGGAGGCCTGTGGGAGCCTCCCGCCCAGCTCAGACCCAGGGCTCTCTGCCCCGCCGACTC
  CTGCCCCCATTCTGCGGGCAGACCTGGCCGGGATCTTGGGGGTCTCAGGAGTCCTCCTCTTTGGCTGTGT
  CTACCTCCTTCATCTGCTGCGCCGACATAAGCACCGCCCTGCCCCTAGGCTCCAGCCGTCCCGCACCAGC
  CCCCAGGCACCGAGAGCACGAGCATGGGCACCAAGCCAGGCCTCCCAGGCTGCTCTTCACGTCCCTTATG
  CCACTATCAACACCAGCTGCCGCCCAGCTACTTTGGACACAGCTCACCCCCATGGGGGGCCGTCCTGGTG
  GGCGTCACTCCCCACCCACGCTGCACACCGGCCCCAGGGCCCTGCCGCCTGGGCCTCCACACCCATCCCT
  GCACGTGGCAGCTTTGTCTCTGTTGAGAATGGACTCTACGCTCAGGCAGGGGAGAGGCCTCCTCACACTG
  GTCCCGGCCTCACTCTTTTCCCTGACCCTCGGGGGCCCAGGGCCATGGAAGGACCCTTAGGAGTTCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210470 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRTEAQVPALQPPEPGLEGAMGHRTLVLPWVLLTLCVTAGTPEVWVQVRMEATELSSFTIRCGFLGSGSI
  SLVTVSWGGPNGAGGTTLAVLHPERGIRQWAPARQARWETQSSISLILEGSGASSPCANTTFCCKFASFP
  EGSWEACGSLPPSSDPGLSAPPTPAPILRADLAGILGVSGVLLFGCVYLLHLLRRHKHRPAPRLQPSRTS
  PQAPRARAWAPSQASQAALHVPYATINTSCRPATLDTAHPHGGPSWWASLPTHAAHRPQGPAAWASTPIP
  ARGSFVSVENGLYAQAGERPPHTGPGLTLFPDPRGPRAMEGPLGVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024070
• ORF Size: 978 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024070.1, NM_024070.2, NM_024070.3, NP_076975.2
• RefSeq Size: 1587 bp
• RefSeq ORF: 981 bp
• Locus ID: 79037
• Protein Families: Transmembrane
• MW: 34.3 kDa