TMEM165 (NM_018475) Human Tagged ORF Clone

CAT#: RC210474

TMEM165 (Myc-DDK-tagged)-Human transmembrane protein 165 (TMEM165)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM165
• Synonyms: CDG2K; FT27; GDT1; TMPT27; TPARL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210474 representing NM_018475
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGCGGCTCCAGGGAACGGCCGCGCATCGGCGCCCCGGCTGCTTCTGCTCTTTCTGGTTCCGC
  TGCTGTGGGCCCCGGCTGCGGTCCGGGCCGGCCCAGATGAAGACCTTAGCCACCGGAACAAAGAACCGCC
  GGCGCCGGCCCAGCAGCTGCAGCCGCAGCCTGTGGCTGTGCAGGGCCCCGAGCCGGCCCGGGTCGAGAAA
  ATATTTACACCAGCAGCTCCAGTTCATACCAATAAAGAAGATCCTGCTACCCAAACTAATTTGGGATTTA
  TCCATGCATTTGTCGCTGCCATATCAGTTATTATTGTATCTGAATTGGGTGATAAGACATTTTTTATAGC
  AGCCATCATGGCAATGCGCTATAACCGCCTGACCGTGCTGGCTGGTGCAATGCTTGCCTTGGGACTAATG
  ACATGCTTGTCAGTTTTGTTTGGCTATGCCACCACAGTCATCCCCAGGGTCTATACATACTATGTTTCAA
  CTGTATTATTTGCCATTTTTGGCATTAGAATGCTTCGGGAAGGCTTAAAGATGAGCCCTGATGAGGGTCA
  AGAGGAACTGGAAGAAGTTCAAGCTGAATTAAAGAAGAAAGATGAAGAATTTCAACGAACCAAACTTTTA
  AATGGACCGGGAGATGTTGAAACGGGTACAAGCATAACAGTACCTCAGAAAAAGTGGTTGCATTTTATTT
  CACCCATTTTTGTTCAAGCTCTTACATTAACATTCTTAGCAGAATGGGGTGATCGCTCTCAACTAACTAC
  AATTGTATTGGCAGCTAGAGAGGACCCCTATGGTGTAGCCGTGGGTGGAACTGTGGGGCACTGCCTGTGC
  ACGGGATTGGCAGTAATTGGAGGAAGAATGATAGCACAGAAAATCTCTGTCAGAACTGTGACAATCATAG
  GAGGCATCGTTTTTTTGGCGTTTGCATTTTCTGCACTATTTATAAGCCCTGATTCTGGTTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210474 representing NM_018475
  Red=Cloning site Green=Tags(s)
  
  MAAAAPGNGRASAPRLLLLFLVPLLWAPAAVRAGPDEDLSHRNKEPPAPAQQLQPQPVAVQGPEPARVEK
  IFTPAAPVHTNKEDPATQTNLGFIHAFVAAISVIIVSELGDKTFFIAAIMAMRYNRLTVLAGAMLALGLM
  TCLSVLFGYATTVIPRVYTYYVSTVLFAIFGIRMLREGLKMSPDEGQEELEEVQAELKKKDEEFQRTKLL
  NGPGDVETGTSITVPQKKWLHFISPIFVQALTLTFLAEWGDRSQLTTIVLAAREDPYGVAVGGTVGHCLC
  TGLAVIGGRMIAQKISVRTVTIIGGIVFLAFAFSALFISPDSGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018475
• ORF Size: 972 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018475.1, NM_018475.2, NM_018475.3, NM_018475.4, NP_060945.2
• RefSeq Size: 1715
• RefSeq ORF: 975
• Locus ID: 55858
• Domains: UPF0016
• Protein Families: Transmembrane
• MW: 34.7 kDa
• Gene Summary: This gene encodes a predicted transmembrane protein with a perinuclear Golgi-like distribution in fibroblasts. Mutations in this gene are associated with the autosomal recessive disorder congenital disorder of glycosylation, type IIk. Knockdown of this gene's expression causes decreased sialylation in HEK cells and suggests this gene plays a role in terminal Golgi glycosylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]