Nucleoside phosphorylase (PNP) (NM_000270) Human Tagged ORF Clone
CAT#: RC210759L4
- LentiORF®
Lenti ORF clone of Human purine nucleoside phosphorylase (PNP), mGFP tagged
"NM_000270" in other vectors (5)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | PNP |
Synonyms | NP; PRO1837; PUNP |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC210759).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_000270 |
ORF Size | 867 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000270.1, NP_000261.1 |
RefSeq Size | 2438 bp |
RefSeq ORF | 870 bp |
Locus ID | 4860 |
Cytogenetics | 14q11.2 |
Domains | Mtap_PNP |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism |
MW | 32.1 kDa |
Gene Summary | 'This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC120008 | PNP (untagged)-Human purine nucleoside phosphorylase (PNP) |
USD 660.00 |
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SC322018 | PNP (untagged)-Human purine nucleoside phosphorylase (PNP) |
USD 660.00 |
|
RC210759 | PNP (Myc-DDK-tagged)-Human purine nucleoside phosphorylase (PNP) |
USD 420.00 |
|
RG210759 | PNP (GFP-tagged) - Human purine nucleoside phosphorylase (PNP) |
USD 460.00 |
|
RC210759L3 | Lenti ORF clone of Human purine nucleoside phosphorylase (PNP), Myc-DDK-tagged |
USD 620.00 |
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