Nucleoside phosphorylase (PNP) (NM_000270) Human Untagged Clone
CAT#: SC322018
PNP (untagged)-Human purine nucleoside phosphorylase (PNP)
"NM_000270" in other vectors (5)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | PNP |
Synonyms | NP; PRO1837; PUNP |
Vector | pCMV6-AC |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>OriGene sequence for SC322018
GCCAGAGCCTAGACCAGTGAGCCAACTGTGCGAACCAGACCCGGCAGCCTTGCTCAGTTC
AGCATAGCGGAGCGGATCCGATCGGATCGGAGCGGATCGGAGCACACCGGAGCAGGCTCA TCGAGAAGGCGTCTGCGAGACCATGGAGAACGGATACACCTATGAAGATTATAAGAACAC TGCAGAATGGCTTCTGTCTCACACTAAGCACCGACCTCAAGTTGCAATAATCTGTGGTTC TGGATTAGGAGGTCTGACTGATAAATTAACTCAGGCCCAGATCTTTGACTACGGTGAAAT CCCCAACTTTCCCCGAAGTACAGTGCCAGGTCATGCTGGCCGACTGGTGTTTGGGTTCCT GAATGGCAGGGCCTGTGTGATGATGCAGGGCAGGTTCCACATGTATGAAGGGTACCCACT CTGGAAGGTGACATTCCCAGTGAGGGTTTTCCACCTTCTGGGTGTGGACACCCTGGTAGT CACCAATGCAGCAGGAGGGCTGAACCCCAAGTTTGAGGTTGGAGATATCATGCTGATCCG TGACCATATCAACCTACCTGGTTTCAGTGGTCAGAACCCTCTCAGAGGGCCCAATGATGA AAGGTTTGGAGATCGTTTCCCTGCCATGTCTGATGCCTACGACCGGACTATGAGGCAGAG GGCTCTCAGTACCTGGAAACAAATGGGGGAGCAACGTGAGCTACAGGAAGGCACCTATGT GATGGTGGCAGGCCCCAGCTTTGAGACTGTGGCAGAATGTCGTGTGCTGCAGAAGCTGGG AGCAGACGCTGTTGGCATGAGTACAGTACCAGAAGTTATCGTTGCACGGCACTGTGGACT TCGAGTCTTTGGCTTCTCACTCATCACTAACAAGGTCATCATGGATTATGAAAGCCTGGA GAAGGCCAACCATGAAGAAGTCTTAGCAGCTGGCAAACAAGCTGCACAGAAATTGGAACA GTTTGTCTCCATTCTTATGGCCAGCATTCCACTCCCTGACAAAGCCAGTTGACCTGCCTT GGAGTCGTCTGGCATCTCCCACACAAGACCCAAGTAGCTGCTACCTTCTTTGGCCCCTTG CTGGAGTCATGTGCCTCTGTCCTTAGGTTGTAGCAGAAAGGAAAAGATTCCTGTCCTTCA CCTTTCCCACTTTCTTCTACCAGACCCTTCTGGTGCCAGATCCTCTTCTCAAAGCTGGGA TTACAGGTGTGAGCATAGTGAGACCTTGGCGCTACAAAATAAAGCTGTTCTCATTCCTGT TCTTTCTTACACAAGAGCTGGAGCCCGTGCCCTACCACACATCTGTGGAGATGCCCAGGA TTTGACTCGGGCCTTAGAACTTTGCATAGCAGCTGCTACTAGCTCTTTGAGATAATACAT TCCGAGGGGCTCAGTTCTGCCTTATCTAAATCACCAGAGACCAAACAAGGACTAATCCAA TACCTCTTGGATTTTATTTAATGTCATAATGTTGTCAGAATAAAGAGAAAGATGAAATAA AAAAAAAAAAAAAA |
Restriction Sites | Please inquire |
ACCN | NM_000270 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000270.1, NP_000261.1 |
RefSeq Size | 1418 bp |
RefSeq ORF | 870 bp |
Locus ID | 4860 |
Cytogenetics | 14q11.2 |
Domains | Mtap_PNP |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism |
Gene Summary | 'This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC120008 | PNP (untagged)-Human purine nucleoside phosphorylase (PNP) |
USD 660.00 |
|
RC210759 | PNP (Myc-DDK-tagged)-Human purine nucleoside phosphorylase (PNP) |
USD 420.00 |
|
RG210759 | PNP (GFP-tagged) - Human purine nucleoside phosphorylase (PNP) |
USD 460.00 |
|
RC210759L3 | Lenti ORF clone of Human purine nucleoside phosphorylase (PNP), Myc-DDK-tagged |
USD 620.00 |
|
RC210759L4 | Lenti ORF clone of Human purine nucleoside phosphorylase (PNP), mGFP tagged |
USD 620.00 |
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