ENT2 (SLC29A2) (NM_001532) Human Tagged ORF Clone

CAT#: RC210776

SLC29A2 (Myc-DDK-tagged)-Human solute carrier family 29 (nucleoside transporters), member 2 (SLC29A2)

Product Images

Western blot validation of overexpression lysate (Cat# LY400588) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210776 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SLC29A2 protein (Cat# TP310776). The protein was produced from HEK293T cells transfected with SLC29A2 cDNA clone (Cat# RC210776) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC29A2
• Synonyms: DER12; ENT2; HNP36
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210776 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGAGGAGACGCCCCGCGGGACAGCTACCACCTGGTCGGGATCAGCTTCTTCATCCTGGGGCTGG
  GCACCCTCCTTCCCTGGAACTTCTTCATCACCGCCATCCCGTACTTCCAGGCGCGACTGGCCGGGGCCGG
  CAACAGCACAGCCAGGATCCTGAGCACCAACCACACGGGTCCCGAGGATGCCTTCAACTTCAACAATTGG
  GTGACGCTGCTGTCCCAGCTGCCCCTGCTGCTCTTCACCCTCCTCAACTCCTTCCTGTACCAGTGCGTCC
  CGGAGACGGTGCGCATTCTGGGCAGCCTGCTGGCCATACTGCTGCTCTTTGCCCTGACAGCAGCGCTGGT
  CAAGGTGGACATGAGCCCCGGACCCTTCTTCTCCATCACCATGGCCTCCGTCTGCTTCATCAACTCCTTC
  AGTGCAGTCCTACAGGGCAGCCTCTTCGGGCAGCTGGGCACCATGCCCTCCACCTACAGCACCCTCTTCC
  TCAGCGGCCAGGGCCTGGCTGGGATCTTTGCTGCCCTTGCCATGCTCCTGTCCATGGCCAGTGGCGTGGA
  CGCCGAGACCTCTGCCCTGGGGTACTTTATCACGCCCTGTGTGGGCATCCTCATGTCCATCGTGTGTTAC
  CTGAGCCTGCCTCACCTGAAGTTTGCCCGCTACTACCTGGCCAATAAATCATCCCAGGCCCAAGCTCAGG
  AGCTGGAGACCAAAGCTGAGCTCCTCCAGTCTGATGAGAACGGGATTCCCAGTAGTCCCCAGAAAGTAGC
  TCTGACCCTGGATCTTGACCTGGAGAAGGAGCCGGAATCAGAGCCAGATGAGCCCCAGAAGCCAGGAAAA
  CCTTCAGTCTTCACTGTCTTCCAGAAGATCTGGCTGACAGCGCTGTGCCTTGTGTTGGTCTTCACAGTCA
  CCCTGTCCGTCTTCCCCGCCATCACAGCCATGGTGACCAGCTCCACCAGTCCTGGGAAGTGGAGTCAGTT
  CTTCAACCCCATCTGCTGCTTCCTCCTCTTCAACATCATGGACTGGCTGGGACGGAGCCTGACCTCTTAC
  TTCCTGTGGCCAGACGAGGACAGCCGGCTGCTGCCCCTGCTGGTCTGCCTGCGGTTCCTGTTCGTGCCCC
  TCTTCATGCTGTGCCACGTGCCCCAGAGGTCCCGGCTGCCCATCCTCTTCCCACAGGATGCCTACTTCAT
  CACCTTCATGCTGCTCTTTGCCGTTTCTAATGGCTACCTGGTGTCCCTCACCATGTGCCTGGCGCCCAGG
  CAGGTGCTGCCACACGAGAGGGAGGTGGCCGGCGCCCTCATGACCTTCTTCCTGGCCCTGGGACTTTCCT
  GTGGAGCCTCCCTCTCCTTCCTCTTCAAGGCGCTGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210776 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARGDAPRDSYHLVGISFFILGLGTLLPWNFFITAIPYFQARLAGAGNSTARILSTNHTGPEDAFNFNNW
  VTLLSQLPLLLFTLLNSFLYQCVPETVRILGSLLAILLLFALTAALVKVDMSPGPFFSITMASVCFINSF
  SAVLQGSLFGQLGTMPSTYSTLFLSGQGLAGIFAALAMLLSMASGVDAETSALGYFITPCVGILMSIVCY
  LSLPHLKFARYYLANKSSQAQAQELETKAELLQSDENGIPSSPQKVALTLDLDLEKEPESEPDEPQKPGK
  PSVFTVFQKIWLTALCLVLVFTVTLSVFPAITAMVTSSTSPGKWSQFFNPICCFLLFNIMDWLGRSLTSY
  FLWPDEDSRLLPLLVCLRFLFVPLFMLCHVPQRSRLPILFPQDAYFITFMLLFAVSNGYLVSLTMCLAPR
  QVLPHEREVAGALMTFFLALGLSCGASLSFLFKALL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001532
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001532.1, NM_001532.2, NP_001523.2
• RefSeq Size: 2529 bp
• RefSeq ORF: 1371 bp
• Locus ID: 3177
• Cytogenetics: 11q13.2
• Protein Families: Transmembrane
• MW: 50.1 kDa
• Gene Summary: 'The uptake of nucleosides by transporters, such as SLC29A2, is essential for nucleotide synthesis by salvage pathways in cells that lack de novo biosynthetic pathways. Nucleoside transport also plays a key role in the regulation of many physiologic processes through its effect on adenosine concentration at the cell surface (Griffiths et al., 1997 [PubMed 9396714]).[supplied by OMIM, Nov 2008]'