TRPC6 (NM_004621) Human Tagged ORF Clone
CAT#: RC210783
- TrueORF®
TRPC6 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 6 (TRPC6)
"NM_004621" in other vectors (6)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | TRPC6 |
Synonyms | FSGS2; TRP6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210783 representing NM_004621
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCAGAGCCCGGCGTTCGGGCCCCGGAGGGGCAGTTCTCCCCGGGGCGCTGCCGGAGCCGCTGCGC GGCGCAACGAGAGCCAGGACTATCTGCTCATGGACTCGGAGCTGGGAGAAGACGGCTGCCCGCAAGCCCC GCTGCCTTGCTACGGCTACTACCCCTGCTTCCGGGGATCTGACAACAGACTGGCTCACCGGCGGCAGACA GTTCTCCGTGAGAAGGGGAGAAGGTTAGCTAATCGAGGACCAGCATACATGTTTAGTGATCGCTCCACAA GCCTATCTATAGAGGAGGAACGCTTTTTGGATGCAGCTGAATATGGTAACATCCCAGTGGTGCGGAAGAT GTTAGAAGAATGCCACTCACTCAACGTTAACTGTGTGGATTACATGGGCCAGAATGCCCTACAGTTGGCA GTGGCCAATGAGCATCTGGAAATTACAGAACTTCTTCTCAAGAAAGAAAACCTCTCTCGAGTTGGGGATG CTTTGCTTCTAGCTATTAGTAAAGGTTATGTTCGGATTGTGGAAGCAATTCTCAGTCATCCGGCTTTTGC TGAAGGCAAGAGGTTAGCAACCAGCCCTAGCCAGTCTGAACTCCAGCAAGATGATTTTTATGCCTATGAT GAAGATGGGACACGGTTCTCCCATGATGTGACTCCAATCATTCTGGCTGCCCACTGCCAGGAATATGAAA TTGTGCATACCCTCCTGCGGAAGGGTGCTAGGATTGAACGGCCTCATGATTATTTCTGCAAGTGCAATGA CTGCAACCAGAAACAGAAGCATGACTCGTTTAGCCACTCCAGATCTAGGATTAATGCCTATAAAGGCCTG GCAAGTCCGGCTTACCTGTCATTGTCTAGTGAAGATCCAGTCATGACGGCTTTAGAACTTAGCAATGAAC TGGCAGTTCTGGCCAATATTGAGAAAGAGTTCAAGAATGACTACAAAAAACTGTCAATGCAGTGCAAAGA CTTTGTTGTTGGACTCCTTGATCTGTGCAGAAACACTGAAGAAGTCGAGGCCATTCTGAATGGGGATGTT GAAACGCTCCAGAGTGGTGATCACGGTCGCCCAAATCTCAGCCGTTTAAAACTTGCCATTAAATATGAAG TAAAAAAATTTGTAGCTCATCCAAACTGCCAACAGCAACTTCTCTCCATTTGGTATGAGAATCTTTCTGG TTTACGACAGCAGACAATGGCGGTCAAGTTCCTTGTGGTCCTTGCTGTTGCCATTGGACTGCCCTTCCTG GCTCTCATTTACTGGTTTGCTCCATGCAGCAAGATGGGGAAGATAATGCGTGGACCATTCATGAAGTTTG TAGCACACGCAGCCTCCTTCACCATTTTTCTGGGACTGCTAGTCATGAATGCAGCTGACAGATTTGAAGG CACAAAACTCCTTCCTAATGAAACCAGCACAGATAATGCAAAACAGCTGTTCAGGATGAAAACATCCTGC TTCTCATGGATGGAGATGCTCATTATATCCTGGGTAATAGGCATGATATGGGCTGAATGTAAAGAAATCT GGACTCAGGGCCCCAAGGAATATTTGTTTGAGTTGTGGAACATGCTTGATTTTGGTATGTTAGCAATTTT CGCAGCATCATTCATTGCGAGATTCATGGCATTTTGGCATGCTTCCAAAGCCCAGAGCATCATTGACGCA AACGATACTTTGAAGGACTTGACGAAAGTAACATTGGGAGACAATGTGAAATACTACAATTTGGCCAGGA TAAAGTGGGACCCCTCTGATCCTCAAATAATATCTGAAGGTCTTTATGCAATTGCTGTAGTTTTAAGTTT CTCTAGGATAGCTTATATTTTACCAGCAAATGAAAGCTTTGGACCTCTGCAGATATCACTTGGAAGAACA GTCAAAGACATCTTCAAGTTCATGGTCATATTCATTATGGTGTTTGTGGCCTTTATGATTGGAATGTTCA ATCTCTACTCCTACTACATTGGTGCAAAACAAAATGAAGCCTTCACAACAGTTGAAGAGAGTTTTAAGAC ACTGTTCTGGGCTATATTTGGACTTTCTGAAGTGAAATCAGTGGTCATCAACTATAACCACAAATTCATT GAAAACATTGGTTACGTTCTTTATGGAGTCTATAATGTTACGATGGTCATTGTTTTGCTAAATATGTTAA TTGCCATGATCAACAGTTCATTCCAGGAAATTGAGGATGACGCTGATGTGGAGTGGAAATTTGCAAGGGC CAAACTCTGGTTTTCCTACTTTGAGGAGGGCAGAACACTTCCTGTACCCTTCAATCTGGTGCCGAGTCCA AAGTCCCTGTTTTATCTCTTACTGAAGCTTAAAAAATGGATTTCTGAGCTGTTCCAGGGCCATAAAAAAG GTTTCCAGGAAGATGCAGAGATGAACAAGATAAATGAAGAAAAGAAACTTGGAATTTTAGGAAGTCATGA AGACCTTTCAAAATTATCACTTGACAAAAAACAGGTTGGGCACAATAAACAACCAAGTATAAGGAGCTCA GAAGATTTCCATCTAAATAGTTTCAATAATCCTCCAAGACAATATCAGAAAATAATGAAAAGGCTCATTA AAAGATATGTACTGCAGGCCCAGATAGATAAGGAGAGTGATGAAGTGAACGAAGGGGAACTGAAGGAAAT TAAGCAGGACATCTCAAGTCTCCGCTATGAACTCCTTGAAGAAAAATCTCAGAATACAGAAGACCTAGCA GAACTTATTAGAGAACTTGGAGAGAAATTATCCATGGAACCAAATCAAGAGGAAACCAATAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210783 representing NM_004621
Red=Cloning site Green=Tags(s) MSQSPAFGPRRGSSPRGAAGAAARRNESQDYLLMDSELGEDGCPQAPLPCYGYYPCFRGSDNRLAHRRQT VLREKGRRLANRGPAYMFSDRSTSLSIEEERFLDAAEYGNIPVVRKMLEECHSLNVNCVDYMGQNALQLA VANEHLEITELLLKKENLSRVGDALLLAISKGYVRIVEAILSHPAFAEGKRLATSPSQSELQQDDFYAYD EDGTRFSHDVTPIILAAHCQEYEIVHTLLRKGARIERPHDYFCKCNDCNQKQKHDSFSHSRSRINAYKGL ASPAYLSLSSEDPVMTALELSNELAVLANIEKEFKNDYKKLSMQCKDFVVGLLDLCRNTEEVEAILNGDV ETLQSGDHGRPNLSRLKLAIKYEVKKFVAHPNCQQQLLSIWYENLSGLRQQTMAVKFLVVLAVAIGLPFL ALIYWFAPCSKMGKIMRGPFMKFVAHAASFTIFLGLLVMNAADRFEGTKLLPNETSTDNAKQLFRMKTSC FSWMEMLIISWVIGMIWAECKEIWTQGPKEYLFELWNMLDFGMLAIFAASFIARFMAFWHASKAQSIIDA NDTLKDLTKVTLGDNVKYYNLARIKWDPSDPQIISEGLYAIAVVLSFSRIAYILPANESFGPLQISLGRT VKDIFKFMVIFIMVFVAFMIGMFNLYSYYIGAKQNEAFTTVEESFKTLFWAIFGLSEVKSVVINYNHKFI ENIGYVLYGVYNVTMVIVLLNMLIAMINSSFQEIEDDADVEWKFARAKLWFSYFEEGRTLPVPFNLVPSP KSLFYLLLKLKKWISELFQGHKKGFQEDAEMNKINEEKKLGILGSHEDLSKLSLDKKQVGHNKQPSIRSS EDFHLNSFNNPPRQYQKIMKRLIKRYVLQAQIDKESDEVNEGELKEIKQDISSLRYELLEEKSQNTEDLA ELIRELGEKLSMEPNQEETNR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004621 |
ORF Size | 2793 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004621.1, NM_004621.2, NM_004621.3, NM_004621.4, NM_004621.5, NP_004612.2 |
RefSeq Size | 4564 bp |
RefSeq ORF | 2796 bp |
Locus ID | 7225 |
Cytogenetics | 11q22.1 |
Domains | ANK, ion_trans |
Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
MW | 106.1 kDa |
Gene Summary | 'The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). [provided by RefSeq, Mar 2009]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC310040 | TRPC6 (untagged)-Human transient receptor potential cation channel, subfamily C, member 6 (TRPC6) |
USD 760.00 |
|
RG210783 | TRPC6 (GFP-tagged) - Human transient receptor potential cation channel, subfamily C, member 6 (TRPC6) |
USD 460.00 |
|
RC210783L1 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 6 (TRPC6), Myc-DDK-tagged |
USD 768.00 |
|
RC210783L2 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 6 (TRPC6), mGFP tagged |
USD 620.00 |
|
RC210783L3 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 6 (TRPC6), Myc-DDK-tagged |
USD 768.00 |
|
RC210783L4 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 6 (TRPC6), mGFP tagged |
USD 768.00 |
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