TRPC6 (NM_004621) Human Untagged Clone

CAT#: SC310040

TRPC6 (untagged)-Human transient receptor potential cation channel, subfamily C, member 6 (TRPC6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRPC6
• Synonyms: FSGS2; TRP6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004621 edited
  CCCGCTGGAACTGCCCACTCGGCTCCCCCGGGAGCGGGGCCCAGGCCAGTCGGGCGTTCC
  CGCCATGAGCCAGAGCCCGGCGTTCGGGCCCCGGAGGGGCAGTTCTCCCCGGGGCGCTGC
  CGGAGCCGCTGCGCGGCGCAACGAGAGCCAGGACTATCTGCTCATGGACTCGGAGCTGGG
  AGAAGACGGCTGCCCGCAAGCCCCGCTGCCTTGCTACGGCTACTACCCCTGCTTCCGGGG
  ATCTGACAACAGACTGGCTCACCGGCGGCAGACAGTTCTCCGTGAGAAGGGGAGAAGGTT
  AGCTAATCGAGGACCAGCATACATGTTTAGTGATCGCTCCACAAGCCTATCTATAGAGGA
  GGAACGCTTTTTGGATGCAGCTGAATATGGTAACATCCCAGTGGTGCGGAAGATGTTAGA
  AGAATGCCACTCACTCAACGTTAACTGTGTGGATTACATGGGCCAGAATGCCCTACAGTT
  GGCAGTGGCCAATGAGCATCTGGAAATTACAGAACTTCTTCTCAAGAAAGAAAACCTCTC
  TCGAGTTGGGGATGCTTTGCTTCTAGCTATTAGTAAAGGTTATGTTCGGATTGTGGAAGC
  AATTCTCAGTCATCCGGCTTTTGCTGAAGGCAAGAGGTTAGCAACCAGCCCTAGCCAGTC
  TGAACTCCAGCAAGATGATTTTTATGCCTATGATGAAGATGGGACACGGTTCTCCCATGA
  TGTGACTCCAATCATTCTGGCTGCCCACTGCCAGGAATATGAAATTGTGCATACCCTCCT
  GCGGAAGGGTGCTAGGATTGAACGGCCTCATGATTATTTCTGCAAGTGCAATGACTGCAA
  CCAGAAACAGAAGCATGACTCGTTTAGCCACTCCAGATCTAGGATTAATGCCTATAAAGG
  CCTGGCAAGTCCGGCTTACCTGTCATTGTCTAGTGAAGATCCAGTCATGACGGCTTTAGA
  ACTTAGCAATGAACTGGCAGTTCTGGCCAATATTGAGAAAGAGTTCAAGAATGACTACAA
  AAAACTGTCAATGCAGTGCAAAGACTTTGTTGTTGGACTCCTTGATCTGTGCAGAAACAC
  TGAAGAAGTCGAGGCCATTCTGAATGGGGATGTTGAAACGCTCCAGAGTGGTGATCACGG
  TCGCCCAAATCTCAGCCGTTTAAAACTTGCCATTAAATATGAAGTAAAAAAATTTGTAGC
  TCATCCAAACTGCCAACAGCAACTTCTCTCCATTTGGTATGAGAATCTTTCTGGTTTACG
  ACAGCAGACAATGGCGGTCAAGTTCCTTGTGGTCCTTGCTGTTGCCATTGGACTGCCCTT
  CCTGGCTCTCATTTACTGGTTTGCTCCATGCAGCAAGATGGGGAAGATAATGCGTGGACC
  ATTCATGAAGTTTGTAGCACACGCAGCCTCCTTCACCATTTTTCTGGGACTGCTAGTCAT
  GAATGCAGCTGACAGATTTGAAGGCACAAAACTCCTTCCTAATGAAACCAGCACAGATAA
  TGCAAAACAGCTGTTCAGGATGAAAACATCCTGCTTCTCATGGATGGAGATGCTCATTAT
  ATCCTGGGTAATAGGCATGATATGGGCTGAATGTAAAGAAATCTGGACTCAGGGCCCCAA
  GGAATATTTGTTTGAGTTGTGGAACATGCTTGATTTTGGTATGTTAGCAATTTTCGCAGC
  ATCATTCATTGCGAGATTCATGGCATTTTGGCATGCTTCCAAAGCCCAGAGCATCATTGA
  CGCAAACGATACTTTGAAGGACTTGACGAAAGTAACATTGGGAGACAATGTGAAATACTA
  CAATTTGGCCAGGATAAAGTGGGACCCCTCTGATCCTCAAATAATATCTGAAGGTCTTTA
  TGCAATTGCTGTAGTTTTAAGTTTCTCTAGGATAGCTTATATTTTACCAGCAAATGAAAG
  CTTTGGACCTCTGCAGATATCACTTGGAAGAACAGTCAAAGACATCTTCAAGTTCATGGT
  CATATTCATTATGGTGTTTGTGGCCTTTATGATTGGAATGTTCAATCTCTACTCCTACTA
  CATTGGTGCAAAACAAAATGAAGCCTTCACAACAGTTGAAGAGAGTTTTAAGACACTGTT
  CTGGGCTATATTTGGACTTTCTGAAGTGAAATCAGTGGTCATCAACTATAACCACAAATT
  CATTGAAAACATTGGTTACGTTCTTTATGGAGTCTATAATGTTACGATGGTCATTGTTTT
  GCTAAATATGTTAATTGCCATGATCAACAGTTCATTCCAGGAAATTGAGGATGACGCTGA
  TGTGGAGTGGAAATTTGCAAGGGCCAAACTCTGGTTTTCCTACTTTGAGGAGGGCAGAAC
  ACTTCCTGTACCCTTCAATCTGGTGCCGAGTCCAAAGTCCCTGTTTTATCTCTTACTGAA
  GCTTAAAAAATGGATTTCTGAGCTGTTCCAGGGCCATAAAAAAGGTTTCCAGGAAGATGC
  AGAGATGAACAAGATAAATGAAGAAAAGAAACTTGGAATTTTAGGAAGTCATGAAGACCT
  TTCAAAATTATCACTTGACAAAAAACAGGTTGGGCACAATAAACAACCAAGTATAAGGAG
  CTCAGAAGATTTCCATCTAAATAGTTTCAATAATCCTCCAAGACAATATCAGAAAATAAT
  GAAAAGGCTCATTAAAAGATATGTACTGCAGGCCCAGATAGATAAGGAGAGTGATGAAGT
  GAACGAAGGGGAACTGAAGGAAATTAAGCAGGACATCTCAAGTCTCCGCTATGAACTCCT
  TGAAGAAAAATCTCAGAATACAGAAGACCTAGCAGAACTTATTAGAGAACTTGGAGAGAA
  ATTATCCATGGAACCAAATCAAGAGGAAACCAATAGATAATGCGAAGACTTCCTTAGAAA
  TTCATATTTATTTGTCCACTTGAAGCCATATTATTTTCTGATTTATTTTCTTAAGTGCCA
  ATGGGCCCACCTTTTAAACAAGAAAACGTTAAATAACTTGGGCCATCCTATCATCTGGAG
  CCCTAGTATCTAATTTTTTTGGTGATTAAACTCCATTGTTCAGGGTAAAGGCTGTAGATA
  ATGAGGAAAATTATGCCCAGTTGTTTGGTGCTTGTTTTATAAACTGCTTTCTTGGATATA
  ACTAACTCTTGTGATGATGTCATTGCCATGTAGTGTCTGCCTGAAAATGGGTCCCAGCGG
  ACAGGGGCTGACCCACGTTACTCCCCATGCGGTTTTTCCTCTGAAGTTTATTTCAGGTTC
  CTTCTTGCCTGCTCTGTGGATCCCCTGCTGGGGACTCCCAGCTCTGAAATTTGGGAAAAA
  GTAGCCCATGGGCCTTTAGAATGCTTTAATCCTTTCTTTAGAATGCTGTTTAAACACCAT
  TTACCCTACTTATCCCTCAATGCACATGATTGATACCGTTCATACAAAATGGTCTTACAT
  CTATGTAAAATTTTCTGATTCATCTATTTGAAAACATTACACTTAACAATGAAAAAAGTT
  TTTCCTCCACTGAACCCTGGAAACATGGTCCAGTTTTGTGTGTGTGCGTGTGTGTAAATG
  TGTACACACAGACATAAAGTACTTGCCCTATTTAGTTTGTGGCTAATGTGGACACACAAA
  AGCTCTTTATGTTATAAATTTTTATTGTCACTAAAAAATTTTACTGTCTAAATAAGTACC
  TTTTATTGGAGAAAAATCAAAACCCCAAACAAACACTGTGGTTGTTTGGTTCCATTATAG
  CACAATTTTGTGCCATTTCTGGGAGCATTTACAGATGAATCCCCACACTTAGCCATTGAA
  TGTAAAGGGGAAAAATAAGGTGAGAATTTGTAAATACTTATCTGTTATTTTCAATATGTT
  CTATCCTTCTACCCAAATATATAAAACAGGAATTTGCATTCATGTGCATTTACCAAGAGG
  TTGTTGTTGTTACTTACTGATCATGTGAAGTGGTGTCTTAAACAACTAAAAGCGATGAAG
  GTTCATATGTTTACTCAAAGACCATTGGCATTCAGAGGATGCTGGACATTAACTGGAACT
  GCTACTTCCAATTCAATAATGGGAGATTTCAAATGCAAATCTTTAACTTCATCTTAAAGA
  TGAAATGGTTGCAGAAAATCTGTTTAGCTCCAACTTTGGCTTAATTTAAATCAAAGAACA
  TTTATGTAACCAGATCAGAAAATACAGCTGAAAATT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004621 unedited
  TCGACAGCTGTATACGACTTACTATAGGGCGGCCGCGAATTCGCCCTTCCCGCCGGAACC
  GCCCACTCGGCTCCCCCGGGAGCGGGGCCCAGGCCAGTCGGGCGTTCCCGCCATGAGCCA
  GAGCCCGGCGTTCGGGCCCCGGAGGGGCAGTTCTCCCCGGGGCGCTGCCGGAGCCGCTGC
  GCGGCGCAACGAGAGCCAGGACTATCTGCTCATGGACTCGGAGCTGGGAGAAGACGGCTG
  CCCGCAAGCCCCGCTGCCTTGCTACGGCTACTACCCCTGCTTCCGGGGACCTGACAACAG
  ACTGGCTCACCGGCGGCAGACAGTTCTCCGTGAGAAGGGGAGAAGGTTAGCTAATCGAGG
  ACCAGCATACATGTTTAGTGATCGCTCCACAAGCCTATCTATAGAGGAGGAACGCTTTTT
  GGATGCAGCTGAATATGGTAACATCCCAGTGGTGCGGAAGATGTTAGAAGAATGCCACTC
  ACTCAACGTTAACTGTGTGGATTACATGGGCCAGAATGCCCTACAGTTGGCAGTGGCCAA
  TGAGCATCTGGAAATTACAGAACTTCTTCTCAAGAAAGAAAACCTCTCTCGAGTTGGGGA
  TGCTTTGCTTCTAGCTATTAGTAAAGGTTATGTTCGGATTGTGGAAGCAATTCTCAGTCA
  TCCGGCTTTTGCTGAAGGCAAGAGGTTAGCAACCAGCCCTAGCCAGTCTGAACTCCAGCA
  AGATGATTTTTATGCCTATGATGAAGATGGGACACGGTTCTCCCATGATGTGACTCCAAT
  CATTCTGGCTGCCCACTGCCAGGAATATGAAATTGTGCATACCCTCCTGCGGAAGGTGCT
  AGGATTGAACGGCCTCATGATTATTTCTGCAGTGCAATGACTGCACCAGAACAGAGCATG
  ACTCGTTTAGCCCCTCCAGATCTAGGATAATGCCTATAAGGCCTGGCAAGTCCGCTTACC
  TGTCATGT
  
• Restriction Sites: Please inquire
• ACCN: NM_004621
• Insert Size: 4200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004621.3, NP_004612.2
• RefSeq Size: 4564 bp
• RefSeq ORF: 2796 bp
• Locus ID: 7225
• Cytogenetics: 11q22.1
• Domains: ANK, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: 'The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). [provided by RefSeq, Mar 2009]'