CHST3 (NM_004273) Human Tagged ORF Clone

CAT#: RC210796

CHST3 (Myc-DDK-tagged)-Human carbohydrate (chondroitin 6) sulfotransferase 3 (CHST3)

Product Images

Western blot validation of overexpression lysate (Cat# LY418102) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210796 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHST3
• Synonyms: C6ST; C6ST1; HSD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210796 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAAGGACTCACTTTGCCCCAGGACTGCCGGGACTTTGTGCACAGCCTGAAGATGAGAAGCAAAT
  ACGCCCTTTTCTTGGTTTTTGTGGTGATAGTTTTTGTCTTCATCGAAAAGGAAAATAAAATCATATCAAG
  GGTCTCAGACAAGCTGAAGCAGATTCCCCAAGCTCTAGCAGATGCCAACAGCACCGACCCAGCCCTGATC
  TTAGCTGAGAACGCATCTCTCTTGTCCCTGAGCGAGCTCGATTCAGCCTTCTCCCAGCTTCAGAGCCGTC
  TCCGCAACCTCAGCTTGCAGCTGGGCGTGGAGCCAGCCATGGAGGCCGCAGGGGAGGAAGAGGAAGAGCA
  GAGAAAGGAGGAGGAGCCGCCCAGACCGGCCGTGGCGGGGCCCCGGCGCCACGTGCTGCTCATGGCCACC
  ACGCGCACCGGCTCCTCGTTCGTGGGCGAGTTCTTCAACCAGCAGGGCAACATCTTCTACCTCTTCGAGC
  CGCTGTGGCACATCGAGCGCACAGTGTCCTTCGAGCCGGGGGGCGCCAACGCCGCGGGCTCGGCCCTGGT
  GTACCGCGACGTGCTCAAGCAGCTCTTCCTGTGCGACCTGTACGTGCTGGAGCACTTCATCACGCCGCTG
  CCCGAGGACCACCTGACTCAGTTCATGTTCCGCCGGGGCTCCAGCCGCTCCCTGTGCGAGGACCCCGTCT
  GTACGCCCTTCGTCAAGAAGGTCTTCGAGAAGTACCACTGCAAGAACCGCCGCTGCGGCCCCCTCAACGT
  GACGCTGGCCGCAGAGGCCTGCCGCCGCAAGGAGCACATGGCCCTCAAGGCGGTGCGCATCCGGCAGCTG
  GAGTTCCTGCAGCCGCTGGCCGAGGACCCCCGCCTGGACCTGCGCGTCATCCAGCTGGTGCGCGACCCCC
  GGGCCGTGCTGGCCTCGCGCATGGTGGCCTTCGCCGGCAAGTATAAGACCTGGAAGAAGTGGCTGGACGA
  CGAGGGCCAGGACGGCCTGAGGGAAGAGGAGGTGCAGCGGCTGCGGGGCAACTGCGAGAGCATCCGCCTG
  TCCGCGGAGCTGGGGCTGCAGCAGCCCGCCTGGCTGCGGGGCCGCTACATGCTGGTGCGCTACGAGGACG
  TGGCACGCGGGCCGCTGCAGAAGGCCCGCGAGATGTACCGCTTCGCCGGCATCCCCCTGACCCCGCAGGT
  GGAAGACTGGATCCAAAAGAACACGCAGGCGGCCCACGACGGCAGCGGCATCTACTCCACGCAGAAGAAC
  TCCTCGGAGCAGTTCGAGAAGTGGCGCTTCAGCATGCCCTTCAAGCTGGCCCAGGTGGTGCAGGCCGCCT
  GCGGCCCTGCCATGCGCCTCTTCGGCTACAAACTGGCGCGGGACGCCGCCGCCCTCACCAACCGCTCAGT
  CAGCCTGCTGGAGGAGAGGGGCACCTTCTGGGTCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210796 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKGLTLPQDCRDFVHSLKMRSKYALFLVFVVIVFVFIEKENKIISRVSDKLKQIPQALADANSTDPALI
  LAENASLLSLSELDSAFSQLQSRLRNLSLQLGVEPAMEAAGEEEEEQRKEEEPPRPAVAGPRRHVLLMAT
  TRTGSSFVGEFFNQQGNIFYLFEPLWHIERTVSFEPGGANAAGSALVYRDVLKQLFLCDLYVLEHFITPL
  PEDHLTQFMFRRGSSRSLCEDPVCTPFVKKVFEKYHCKNRRCGPLNVTLAAEACRRKEHMALKAVRIRQL
  EFLQPLAEDPRLDLRVIQLVRDPRAVLASRMVAFAGKYKTWKKWLDDEGQDGLREEEVQRLRGNCESIRL
  SAELGLQQPAWLRGRYMLVRYEDVARGPLQKAREMYRFAGIPLTPQVEDWIQKNTQAAHDGSGIYSTQKN
  SSEQFEKWRFSMPFKLAQVVQAACGPAMRLFGYKLARDAAALTNRSVSLLEERGTFWVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004273
• ORF Size: 1437 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004273.1, NM_004273.2, NM_004273.3, NM_004273.4, NP_004264.2
• RefSeq Size: 6978 bp
• RefSeq ORF: 1440 bp
• Locus ID: 9469
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis
• MW: 54.7 kDa
• Gene Summary: This gene encodes an enzyme which catalyzes the sulfation of chondroitin, a proteoglycan found in the extracellular matrix and most cells which is involved in cell migration and differentiation. Mutations in this gene are associated with spondylepiphyseal dysplasia and humerospinal dysostosis. [provided by RefSeq, Mar 2009]