CHST3 (NM_004273) Human Tagged ORF Clone
CAT#: RC210796
CHST3 (Myc-DDK-tagged)-Human carbohydrate (chondroitin 6) sulfotransferase 3 (CHST3)
"NM_004273" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CHST3 |
Synonyms | C6ST; C6ST1; HSD |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210796 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAAGGACTCACTTTGCCCCAGGACTGCCGGGACTTTGTGCACAGCCTGAAGATGAGAAGCAAAT ACGCCCTTTTCTTGGTTTTTGTGGTGATAGTTTTTGTCTTCATCGAAAAGGAAAATAAAATCATATCAAG GGTCTCAGACAAGCTGAAGCAGATTCCCCAAGCTCTAGCAGATGCCAACAGCACCGACCCAGCCCTGATC TTAGCTGAGAACGCATCTCTCTTGTCCCTGAGCGAGCTCGATTCAGCCTTCTCCCAGCTTCAGAGCCGTC TCCGCAACCTCAGCTTGCAGCTGGGCGTGGAGCCAGCCATGGAGGCCGCAGGGGAGGAAGAGGAAGAGCA GAGAAAGGAGGAGGAGCCGCCCAGACCGGCCGTGGCGGGGCCCCGGCGCCACGTGCTGCTCATGGCCACC ACGCGCACCGGCTCCTCGTTCGTGGGCGAGTTCTTCAACCAGCAGGGCAACATCTTCTACCTCTTCGAGC CGCTGTGGCACATCGAGCGCACAGTGTCCTTCGAGCCGGGGGGCGCCAACGCCGCGGGCTCGGCCCTGGT GTACCGCGACGTGCTCAAGCAGCTCTTCCTGTGCGACCTGTACGTGCTGGAGCACTTCATCACGCCGCTG CCCGAGGACCACCTGACTCAGTTCATGTTCCGCCGGGGCTCCAGCCGCTCCCTGTGCGAGGACCCCGTCT GTACGCCCTTCGTCAAGAAGGTCTTCGAGAAGTACCACTGCAAGAACCGCCGCTGCGGCCCCCTCAACGT GACGCTGGCCGCAGAGGCCTGCCGCCGCAAGGAGCACATGGCCCTCAAGGCGGTGCGCATCCGGCAGCTG GAGTTCCTGCAGCCGCTGGCCGAGGACCCCCGCCTGGACCTGCGCGTCATCCAGCTGGTGCGCGACCCCC GGGCCGTGCTGGCCTCGCGCATGGTGGCCTTCGCCGGCAAGTATAAGACCTGGAAGAAGTGGCTGGACGA CGAGGGCCAGGACGGCCTGAGGGAAGAGGAGGTGCAGCGGCTGCGGGGCAACTGCGAGAGCATCCGCCTG TCCGCGGAGCTGGGGCTGCAGCAGCCCGCCTGGCTGCGGGGCCGCTACATGCTGGTGCGCTACGAGGACG TGGCACGCGGGCCGCTGCAGAAGGCCCGCGAGATGTACCGCTTCGCCGGCATCCCCCTGACCCCGCAGGT GGAAGACTGGATCCAAAAGAACACGCAGGCGGCCCACGACGGCAGCGGCATCTACTCCACGCAGAAGAAC TCCTCGGAGCAGTTCGAGAAGTGGCGCTTCAGCATGCCCTTCAAGCTGGCCCAGGTGGTGCAGGCCGCCT GCGGCCCTGCCATGCGCCTCTTCGGCTACAAACTGGCGCGGGACGCCGCCGCCCTCACCAACCGCTCAGT CAGCCTGCTGGAGGAGAGGGGCACCTTCTGGGTCACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210796 protein sequence
Red=Cloning site Green=Tags(s) MEKGLTLPQDCRDFVHSLKMRSKYALFLVFVVIVFVFIEKENKIISRVSDKLKQIPQALADANSTDPALI LAENASLLSLSELDSAFSQLQSRLRNLSLQLGVEPAMEAAGEEEEEQRKEEEPPRPAVAGPRRHVLLMAT TRTGSSFVGEFFNQQGNIFYLFEPLWHIERTVSFEPGGANAAGSALVYRDVLKQLFLCDLYVLEHFITPL PEDHLTQFMFRRGSSRSLCEDPVCTPFVKKVFEKYHCKNRRCGPLNVTLAAEACRRKEHMALKAVRIRQL EFLQPLAEDPRLDLRVIQLVRDPRAVLASRMVAFAGKYKTWKKWLDDEGQDGLREEEVQRLRGNCESIRL SAELGLQQPAWLRGRYMLVRYEDVARGPLQKAREMYRFAGIPLTPQVEDWIQKNTQAAHDGSGIYSTQKN SSEQFEKWRFSMPFKLAQVVQAACGPAMRLFGYKLARDAAALTNRSVSLLEERGTFWVT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004273 |
ORF Size | 1437 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004273.1, NM_004273.2, NM_004273.3, NM_004273.4, NP_004264.2 |
RefSeq Size | 6978 bp |
RefSeq ORF | 1440 bp |
Locus ID | 9469 |
Protein Families | Transmembrane |
Protein Pathways | Chondroitin sulfate biosynthesis |
MW | 54.7 kDa |
Gene Summary | This gene encodes an enzyme which catalyzes the sulfation of chondroitin, a proteoglycan found in the extracellular matrix and most cells which is involved in cell migration and differentiation. Mutations in this gene are associated with spondylepiphyseal dysplasia and humerospinal dysostosis. [provided by RefSeq, Mar 2009] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC303460 | CHST3 (untagged)-Human carbohydrate (chondroitin 6) sulfotransferase 3 (CHST3) |
USD 760.00 |
|
RG210796 | CHST3 (GFP-tagged) - Human carbohydrate (chondroitin 6) sulfotransferase 3 (CHST3) |
USD 460.00 |
|
RC210796L1 | Lenti ORF clone of Human carbohydrate (chondroitin 6) sulfotransferase 3 (CHST3), Myc-DDK-tagged |
USD 768.00 |
|
RC210796L2 | Lenti ORF clone of Human carbohydrate (chondroitin 6) sulfotransferase 3 (CHST3), mGFP tagged |
USD 620.00 |
|
RC210796L3 | Lenti ORF clone of Human carbohydrate (chondroitin 6) sulfotransferase 3 (CHST3), Myc-DDK-tagged |
USD 620.00 |
|
RC210796L4 | Lenti ORF clone of Human carbohydrate (chondroitin 6) sulfotransferase 3 (CHST3), mGFP tagged |
USD 620.00 |
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