CHST3 (NM_004273) Human Tagged ORF Clone

CAT#: RG210796

CHST3 (GFP-tagged) - Human carbohydrate (chondroitin 6) sulfotransferase 3 (CHST3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CHST3
• Synonyms: C6ST; C6ST1; HSD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210796 representing NM_004273
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAAGGACTCACTTTGCCCCAGGACTGCCGGGACTTTGTGCACAGCCTGAAGATGAGAAGCAAAT
  ACGCCCTTTTCTTGGTTTTTGTGGTGATAGTTTTTGTCTTCATCGAAAAGGAAAATAAAATCATATCAAG
  GGTCTCAGACAAGCTGAAGCAGATTCCCCAAGCTCTAGCAGATGCCAACAGCACCGACCCAGCCCTGATC
  TTAGCTGAGAACGCATCTCTCTTGTCCCTGAGCGAGCTCGATTCAGCCTTCTCCCAGCTTCAGAGCCGTC
  TCCGCAACCTCAGCTTGCAGCTGGGCGTGGAGCCAGCCATGGAGGCCGCAGGGGAGGAAGAGGAAGAGCA
  GAGAAAGGAGGAGGAGCCGCCCAGACCGGCCGTGGCGGGGCCCCGGCGCCACGTGCTGCTCATGGCCACC
  ACGCGCACCGGCTCCTCGTTCGTGGGCGAGTTCTTCAACCAGCAGGGCAACATCTTCTACCTCTTCGAGC
  CGCTGTGGCACATCGAGCGCACAGTGTCCTTCGAGCCGGGGGGCGCCAACGCCGCGGGCTCGGCCCTGGT
  GTACCGCGACGTGCTCAAGCAGCTCTTCCTGTGCGACCTGTACGTGCTGGAGCACTTCATCACGCCGCTG
  CCCGAGGACCACCTGACTCAGTTCATGTTCCGCCGGGGCTCCAGCCGCTCCCTGTGCGAGGACCCCGTCT
  GTACGCCCTTCGTCAAGAAGGTCTTCGAGAAGTACCACTGCAAGAACCGCCGCTGCGGCCCCCTCAACGT
  GACGCTGGCCGCAGAGGCCTGCCGCCGCAAGGAGCACATGGCCCTCAAGGCGGTGCGCATCCGGCAGCTG
  GAGTTCCTGCAGCCGCTGGCCGAGGACCCCCGCCTGGACCTGCGCGTCATCCAGCTGGTGCGCGACCCCC
  GGGCCGTGCTGGCCTCGCGCATGGTGGCCTTCGCCGGCAAGTATAAGACCTGGAAGAAGTGGCTGGACGA
  CGAGGGCCAGGACGGCCTGAGGGAAGAGGAGGTGCAGCGGCTGCGGGGCAACTGCGAGAGCATCCGCCTG
  TCCGCGGAGCTGGGGCTGCAGCAGCCCGCCTGGCTGCGGGGCCGCTACATGCTGGTGCGCTACGAGGACG
  TGGCACGCGGGCCGCTGCAGAAGGCCCGCGAGATGTACCGCTTCGCCGGCATCCCCCTGACCCCGCAGGT
  GGAAGACTGGATCCAAAAGAACACGCAGGCGGCCCACGACGGCAGCGGCATCTACTCCACGCAGAAGAAC
  TCCTCGGAGCAGTTCGAGAAGTGGCGCTTCAGCATGCCCTTCAAGCTGGCCCAGGTGGTGCAGGCCGCCT
  GCGGCCCTGCCATGCGCCTCTTCGGCTACAAACTGGCGCGGGACGCCGCCGCCCTCACCAACCGCTCAGT
  CAGCCTGCTGGAGGAGAGGGGCACCTTCTGGGTCACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210796 representing NM_004273
  Red=Cloning site Green=Tags(s)
  
  MEKGLTLPQDCRDFVHSLKMRSKYALFLVFVVIVFVFIEKENKIISRVSDKLKQIPQALADANSTDPALI
  LAENASLLSLSELDSAFSQLQSRLRNLSLQLGVEPAMEAAGEEEEEQRKEEEPPRPAVAGPRRHVLLMAT
  TRTGSSFVGEFFNQQGNIFYLFEPLWHIERTVSFEPGGANAAGSALVYRDVLKQLFLCDLYVLEHFITPL
  PEDHLTQFMFRRGSSRSLCEDPVCTPFVKKVFEKYHCKNRRCGPLNVTLAAEACRRKEHMALKAVRIRQL
  EFLQPLAEDPRLDLRVIQLVRDPRAVLASRMVAFAGKYKTWKKWLDDEGQDGLREEEVQRLRGNCESIRL
  SAELGLQQPAWLRGRYMLVRYEDVARGPLQKAREMYRFAGIPLTPQVEDWIQKNTQAAHDGSGIYSTQKN
  SSEQFEKWRFSMPFKLAQVVQAACGPAMRLFGYKLARDAAALTNRSVSLLEERGTFWVT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004273
• ORF Size: 1437 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004273.2, NP_004264.2
• RefSeq Size: 6961
• RefSeq ORF: 1440
• Locus ID: 9469
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis
• Gene Summary: This gene encodes an enzyme which catalyzes the sulfation of chondroitin, a proteoglycan found in the extracellular matrix and most cells which is involved in cell migration and differentiation. Mutations in this gene are associated with spondylepiphyseal dysplasia and humerospinal dysostosis. [provided by RefSeq, Mar 2009]