AP3B2 (NM_004644) Human Tagged ORF Clone

CAT#: RC210820

AP3B2 (Myc-DDK-tagged)-Human adaptor-related protein complex 3, beta 2 subunit (AP3B2)

Product Images

Western blot validation of overexpression lysate (Cat# LY401473) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210820 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AP3B2
• Synonyms: EIEE48; NAPTB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210820 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCGCCCCCGCCTACAGCGAAGACAAGGGCGGCTCCGCTGGCCCCGGGGAGCCCGAGTACGGCC
  ACGACCCCGCGAGCGGCGGCATCTTCTCCTCCGACTACAAGCGGCATGATGACCTGAAGGAGATGCTGGA
  CACCAACAAGGATTCTCTCAAGCTGGAGGCCATGAAGAGGATTGTGGCGATGATTGCCCGAGGAAAGAAT
  GCTTCAGACCTGTTTCCCGCGGTGGTGAAGAACGTGGCCTGTAAGAACATAGAGGTGAAGAAGCTTGTCT
  ATGTGTACCTGGTACGCTACGCTGAGGAGCAGCAAGACCTGGCCCTGCTGTCCATCTCCACCTTCCAACG
  TGGCCTAAAGGATCCCAACCAGCTGATTCGTGCCAGTGCCCTCCGTGTCCTCTCTAGCATCCGTGTGCCC
  ATCATAGTGCCCATCATGATGCTAGCTATCAAGGAAGCCGCCTCGGACATGTCACCCTATGTGCGGAAAA
  CAGCTGCCCACGCCATCCCTAAACTCTACAGTTTGGACTCTGACCAGAAGGATCAGCTGATAGAAGTCAT
  TGAGAAGCTTCTGGCTGACAAGACCACGCTGGTGGCGGGCAGTGTGGTGATGGCCTTTGAGGAGGTCTGC
  CCGGAGCGCATCGACCTGATTCACAAAAACTACCGGAAACTCTGTAACCTGCTGATCGACGTGGAGGAGT
  GGGGCCAGGTGGTCATCATCAGCATGCTCACCCGCTACGCCCGCACGCAGTTCCTGAGCCCCACCCAGAA
  CGAATCCCTACTAGAGGAGAACGCGGAAAAAGCCTTCTACGGCTCAGAGGAGGACGAGGCCAAGGGCGCG
  GGGTCTGAGGAGACGGCCGCCGCGGCCGCCCCCTCCCGAAAGCCCTATGTCATGGACCCCGACCACCGGC
  TGCTGCTGCGCAACACCAAACCCCTGCTGCAGAGCCGCAGCGCCGCGGTGGTGATGGCGGTGGCGCAGCT
  CTACTTCCACCTGGCGCCCAAGGCGGAAGTGGGCGTCATCGCCAAGGCGCTGGTGCGCCTGCTGCGCAGC
  CACAGTGAGGTGCAGTACGTTGTGCTCCAGAACGTGGCCACCATGTCCATCAAGCGCCGGGGTATGTTTG
  AGCCCTACCTGAAGAGCTTCTACATCAGGTCCACCGACCCCACCCAGATTAAGATCCTGAAGCTGGAAGT
  GCTGACCAACCTGGCCAATGAGACCAACATTCCTACTGTCCTACGGGAATTCCAGACCTATATTCGCAGC
  ATGGACAAGGACTTTGTGGCAGCCACAATCCAGGCCATTGGACGCTGTGCAACTAACATCGGCCGAGTCC
  GTGACACCTGCCTCAATGGCCTGGTGCAGCTGCTGTCCAACCGTGATGAGCTTGTGGTTGCAGAGTCAGT
  GGTCGTCATTAAGAAATTGCTACAGATGCAGCCAGCACAACATGGAGAGATCATCAAACACTTGGCAAAG
  CTTACAGACAACATCCAGGTGCCCATGGCCCGAGCCAGCATCCTGTGGCTCATCGGAGAGTACTGTGAGC
  ATGTCCCCAGGATTGCACCTGATGTCTTAAGAAAAATGGCCAAGTCATTCACAGCAGAGGAGGATATTGT
  CAAGCTGCAGGTCATCAACCTGGCAGCCAAGCTCTACCTGACCAACTCTAAACAGACCAAGCTGCTGACC
  CAGTATGTGCTGAGTCTGGCCAAATATGACCAGAACTATGATATTCGCGACCGGGCGCGCTTCACCCGGC
  AGCTCATCGTCCCTTCCGAGCAGGGTGGGGCCCTCAGCCGCCATGCCAAGAAGCTCTTCCTGGCACCCAA
  ACCAGCTCCAGTCTTGGAGTCATCCTTCAAAGACCGGGACCACTTCCAGCTGGGCTCACTGTCCCACCTG
  CTTAATGCCAAGGCCACAGGCTACCAGGAGCTCCCAGACTGGCCGGAGGAAGCCCCAGACCCATCTGTGC
  GCAACGTGGAGGTACCTGAATGGACCAAGTGCTCAAATCGGGAGAAGAGAAAGGAGAAGGAAAAACCCTT
  CTACTCGGACTCTGAGGGGGAGTCAGGCCCCACGGAGTCCGCAGACAGTGACCCTGAGTCTGAGAGTGAA
  TCGGACAGTAAGAGCAGCAGTGAGAGCGGCTCTGGGGAGTCCAGCAGTGAGTCCGACAATGAAGACCAGG
  ATGAGGATGAGGAGAAAGGGAGAGGCAGTGAGAGTGAACAGAGTGAGGAGGATGGTAAGAGGAAGACAAA
  GAAGAAGGTGCCAGAGAGAAAAGGAGAAGCGTCATCCTCTGATGAGGGCAGCGATTCCAGCAGTAGCTCA
  TCAGAGTCCGAGATGACATCGGAGTCCGAGGAGGAGCAGTTAGAACCTGCCTCCTGGAGCAGGAAAACAC
  CTCCCAGCAGCAAAAGTGCTCCTGCAACCAAGGAGATCTCCCTGCTTGATCTAGAGGATTTCACCCCTCC
  CAGTGTCCAGCCTGTGTCTCCCCCAGCAATTGTGTCTACCAGTCTGGCTGCTGACCTGGAGGGCCTGACA
  CTCACAGACTCCACCCTGGTACCGTCGCTTCTGAGTCCAGTATCGGGTGTTGGGCGGCAGGAGCTGCTGC
  ACCGGGTAGCTGGCGAGGGGCTGGCTGTGGACTACACCTTCAGCCGCCAACCTTTCTCCGGGGATCCCCA
  CATGGTGTCCGTGCACATCCACTTCTCCAACAGCTCTGATACCCCCATCAAGGGCCTGCATGTGGGCACT
  CCCAAACTGCCTGCTGGCATCAGCATCCAAGAATTTCCCGAAATTGAGTCCCTGGCACCTGGAGAATCTG
  CCACTGCTGTAATGGGCATTAATTTCTGTGACTCAACCCAGGCAGCCAACTTCCAGCTGTGCACCCAGAC
  CCGACAGTTCTACGTCTCCATTCAGCCACCTGTTGGGGAGCTGATGGCCCCTGTGTTCATGAGTGAAAAT
  GAGTTTAAGAAGGAACAGGGAAAGCTGATGGGCATGAATGAGATCACAGAGAAACTCATGCTGCCAGACA
  CCTGTCGGAGTGACCACATTGTGGTGCAGAAAGTGACTGCCACTGCCAACCTGGGTCGTGTTCCTTGTGG
  GACATCTGATGAGTACAGGTTTGCAGGGAGGACACTGACTGGTGGAAGCCTCGTTCTGCTGACCCTGGAT
  GCCCGGCCAGCTGGAGCTGCCCAGCTGACTGTCAACAGCGAGAAAATGGTGATTGGCACCATGCTGGTAA
  AGGATGTGATACAGGCTCTGACCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210820 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSAAPAYSEDKGGSAGPGEPEYGHDPASGGIFSSDYKRHDDLKEMLDTNKDSLKLEAMKRIVAMIARGKN
  ASDLFPAVVKNVACKNIEVKKLVYVYLVRYAEEQQDLALLSISTFQRGLKDPNQLIRASALRVLSSIRVP
  IIVPIMMLAIKEAASDMSPYVRKTAAHAIPKLYSLDSDQKDQLIEVIEKLLADKTTLVAGSVVMAFEEVC
  PERIDLIHKNYRKLCNLLIDVEEWGQVVIISMLTRYARTQFLSPTQNESLLEENAEKAFYGSEEDEAKGA
  GSEETAAAAAPSRKPYVMDPDHRLLLRNTKPLLQSRSAAVVMAVAQLYFHLAPKAEVGVIAKALVRLLRS
  HSEVQYVVLQNVATMSIKRRGMFEPYLKSFYIRSTDPTQIKILKLEVLTNLANETNIPTVLREFQTYIRS
  MDKDFVAATIQAIGRCATNIGRVRDTCLNGLVQLLSNRDELVVAESVVVIKKLLQMQPAQHGEIIKHLAK
  LTDNIQVPMARASILWLIGEYCEHVPRIAPDVLRKMAKSFTAEEDIVKLQVINLAAKLYLTNSKQTKLLT
  QYVLSLAKYDQNYDIRDRARFTRQLIVPSEQGGALSRHAKKLFLAPKPAPVLESSFKDRDHFQLGSLSHL
  LNAKATGYQELPDWPEEAPDPSVRNVEVPEWTKCSNREKRKEKEKPFYSDSEGESGPTESADSDPESESE
  SDSKSSSESGSGESSSESDNEDQDEDEEKGRGSESEQSEEDGKRKTKKKVPERKGEASSSDEGSDSSSSS
  SESEMTSESEEEQLEPASWSRKTPPSSKSAPATKEISLLDLEDFTPPSVQPVSPPAIVSTSLAADLEGLT
  LTDSTLVPSLLSPVSGVGRQELLHRVAGEGLAVDYTFSRQPFSGDPHMVSVHIHFSNSSDTPIKGLHVGT
  PKLPAGISIQEFPEIESLAPGESATAVMGINFCDSTQAANFQLCTQTRQFYVSIQPPVGELMAPVFMSEN
  EFKKEQGKLMGMNEITEKLMLPDTCRSDHIVVQKVTATANLGRVPCGTSDEYRFAGRTLTGGSLVLLTLD
  ARPAGAAQLTVNSEKMVIGTMLVKDVIQALTQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004644
• ORF Size: 3246 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004644.1, NM_004644.2, NM_004644.3, NM_004644.4, NP_004635.2
• RefSeq Size: 3771 bp
• RefSeq ORF: 3249 bp
• Locus ID: 8120
• Domains: Adaptin_N
• Protein Pathways: Lysosome
• MW: 119.1 kDa
• Gene Summary: Adaptor protein complex 3 (AP-3 complex) is a heterotrimeric protein complex involved in the formation of clathrin-coated synaptic vesicles. The protein encoded by this gene represents the beta subunit of the neuron-specific AP-3 complex and was first identified as the target antigen in human paraneoplastic neurologic disorders. The encoded subunit binds clathrin and is phosphorylated by a casein kinase-like protein, which mediates synaptic vesicle coat assembly. Defects in this gene are a cause of early-onset epileptic encephalopathy. [provided by RefSeq, Feb 2017]