ADAMTS5 (NM_007038) Human Tagged ORF Clone

CAT#: RC210834

ADAMTS5 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 5 (ADAMTS5)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADAMTS5
• Synonyms: ADAM-TS 5; ADAM-TS5; ADAM-TS 11; ADAMTS-5; ADAMTS-11; ADAMTS11; ADMP-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210834 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTCGGGTGGGCGTCCCTGCTGCTGTGCGCGTTCCGCCTGCCCCTGGCCGCGGTCGGCCCCGCCG
  CGACACCTGCCCAGGATAAAGCCGGGCAGCCTCCGACTGCTGCAGCAGCCGCCCAGCCCCGCCGGCGGCA
  GGGGGAGGAGGTGCAGGAGCGAGCCGAGCCTCCCGGCCACCCGCACCCCCTGGCGCAGCGGCGCAGGAGC
  AAGGGGCTGGTGCAGAACATCGACCAACTCTACTCCGGCGGCGGCAAGGTGGGCTACCTCGTCTACGCGG
  GCGGCCGGAGGTTCCTCTTGGACCTGGAGCGAGATGGTTCGGTGGGCATTGCTGGCTTCGTGCCCGCAGG
  AGGCGGGACGAGTGCGCCCTGGCGCCACCGGAGCCACTGCTTCTATCGGGGCACAGTGGACGCTAGTCCC
  CGCTCTCTGGCTGTCTTTGACCTCTGTGGGGGTCTCGACGGCTTCTTCGCGGTCAAGCACGCGCGCTACA
  CCCTAAAGCCACTGCTGCGCGGACCCTGGGCGGAGGAAGAAAAGGGGCGCGTGTACGGGGATGGGTCCGC
  ACGGATCCTGCACGTCTACACCCGCGAGGGCTTCAGCTTCGAGGCCCTGCCGCCGCGCGCCAGCTGCGAA
  ACCCCCGCGTCCACACCGGAGGCCCACGAGCATGCTCCGGCGCACAGCAACCCGAGCGGACGCGCAGCAC
  TGGCCTCGCAGCTCTTGGACCAGTCCGCTCTCTCGCCCGCTGGGGGCTCAGGACCGCAGACGTGGTGGCG
  GCGGCGGCGCCGCTCCATCTCCCGGGCCCGCCAGGTGGAGCTGCTTCTGGTGGCTGACGCGTCCATGGCG
  CGGTTGTATGGCCGGGGCCTGCAGCATTACCTGCTGACCCTGGCCTCCATCGCCAATAGGCTGTACAGCC
  ATGCTAGCATCGAGAACCACATCCGCCTGGCCGTGGTGAAGGTGGTGGTGCTAGGCGACAAGGACAAGAG
  CCTGGAAGTGAGCAAGAACGCTGCCACCACACTCAAGAACTTTTGCAAGTGGCAGCACCAACACAACCAG
  CTGGGAGATGACCATGAGGAGCACTACGATGCAGCTATCCTGTTTACTCGGGAGGATTTATGTGGGCATC
  ATTCATGTGACACCCTGGGAATGGCAGACGTTGGGACCATATGTTCTCCAGAGCGCAGCTGTGCTGTGAT
  TGAAGACGATGGCCTCCACGCAGCCTTCACTGTGGCTCACGAAATCGGACATTTACTTGGCCTCTCCCAT
  GACGATTCCAAATTCTGTGAAGAGACCTTTGGTTCCACAGAAGATAAGCGCTTAATGTCTTCCATCCTTA
  CCAGCATTGATGCATCTAAGCCCTGGTCCAAATGCACTTCAGCCACCATCACAGAATTCCTGGATGATGG
  CCATGGTAACTGTTTGCTGGACCTACCACGAAAGCAGATCCTGGGCCCCGAAGAACTCCCAGGACAGACC
  TACGATGCCACCCAGCAGTGCAACCTGACATTCGGGCCTGAGTACTCCGTGTGTCCCGGCATGGATGTCT
  GTGCTCGCCTGTGGTGTGCTGTGGTACGCCAGGGCCAGATGGTCTGTCTGACCAAGAAGCTGCCTGCGGT
  GGAAGGGACGCCTTGTGGAAAGGGGAGAATCTGCCTGCAGGGCAAATGTGTGGACAAAACCAAGAAAAAA
  TATTATTCAACGTCAAGCCATGGCAACTGGGGATCTTGGGGATCCTGGGGCCAGTGTTCTCGCTCATGTG
  GAGGAGGAGTGCAGTTTGCCTATCGTCACTGTAATAACCCTGCTCCCAGAAACAACGGACGCTACTGCAC
  AGGGAAGAGGGCCATCTACCGCTCCTGCAGTCTCATGCCCTGCCCACCCAATGGTAAATCATTTCGTCAT
  GAACAGTGTGAGGCCAAAAATGGCTATCAGTCTGATGCAAAAGGAGTCAAAACTTTTGTGGAATGGGTTC
  CCAAATATGCAGGTGTCCTGCCAGCGGATGTGTGCAAGCTGACCTGCAGAGCCAAGGGCACTGGCTACTA
  TGTGGTATTTTCTCCAAAGGTGACCGATGGCACTGAATGTAGGCCGTACAGTAATTCCGTCTGCGTCCGG
  GGGAAGTGTGTGAGAACTGGCTGTGACGGCATCATTGGCTCAAAGCTGCAGTATGACAAGTGCGGAGTAT
  GTGGAGGAGACAACTCCAGCTGTACAAAGATTGTTGGAACCTTTAATAAGAAAAGTAAGGGTTACACTGA
  CGTGGTGAGGATTCCTGAAGGGGCAACCCACATAAAAGTTCGACAGTTCAAAGCCAAAGACCAGACTAGA
  TTCACTGCCTATTTAGCCCTGAAAAAGAAAAACGGTGAGTACCTTATCAATGGAAAGTACATGATCTCCA
  CTTCAGAGACTATCATTGACATCAATGGAACAGTCATGAACTATAGCGGTTGGAGCCACAGGGATGACTT
  CCTGCATGGCATGGGCTACTCTGCCACGAAGGAAATTCTAATAGTGCAGATTCTTGCAACAGACCCCACT
  AAACCATTAGATGTCCGTTATAGCTTTTTTGTTCCCAAGAAGTCCACTCCAAAAGTAAACTCTGTCACTA
  GTCATGGCAGCAATAAAGTGGGATCACACACTTCGCAGCCGCAGTGGGTCACGGGCCCATGGCTCGCCTG
  CTCTAGGACCTGTGACACAGGTTGGCACACCAGAACGGTGCAGTGCCAGGATGGAAACCGGAAGTTAGCA
  AAAGGATGTCCTCTCTCCCAAAGGCCTTCTGCGTTTAAGCAATGCTTGTTGAAGAAATGT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210834 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLLGWASLLLCAFRLPLAAVGPAATPAQDKAGQPPTAAAAAQPRRRQGEEVQERAEPPGHPHPLAQRRRS
  KGLVQNIDQLYSGGGKVGYLVYAGGRRFLLDLERDGSVGIAGFVPAGGGTSAPWRHRSHCFYRGTVDASP
  RSLAVFDLCGGLDGFFAVKHARYTLKPLLRGPWAEEEKGRVYGDGSARILHVYTREGFSFEALPPRASCE
  TPASTPEAHEHAPAHSNPSGRAALASQLLDQSALSPAGGSGPQTWWRRRRRSISRARQVELLLVADASMA
  RLYGRGLQHYLLTLASIANRLYSHASIENHIRLAVVKVVVLGDKDKSLEVSKNAATTLKNFCKWQHQHNQ
  LGDDHEEHYDAAILFTREDLCGHHSCDTLGMADVGTICSPERSCAVIEDDGLHAAFTVAHEIGHLLGLSH
  DDSKFCEETFGSTEDKRLMSSILTSIDASKPWSKCTSATITEFLDDGHGNCLLDLPRKQILGPEELPGQT
  YDATQQCNLTFGPEYSVCPGMDVCARLWCAVVRQGQMVCLTKKLPAVEGTPCGKGRICLQGKCVDKTKKK
  YYSTSSHGNWGSWGSWGQCSRSCGGGVQFAYRHCNNPAPRNNGRYCTGKRAIYRSCSLMPCPPNGKSFRH
  EQCEAKNGYQSDAKGVKTFVEWVPKYAGVLPADVCKLTCRAKGTGYYVVFSPKVTDGTECRPYSNSVCVR
  GKCVRTGCDGIIGSKLQYDKCGVCGGDNSSCTKIVGTFNKKSKGYTDVVRIPEGATHIKVRQFKAKDQTR
  FTAYLALKKKNGEYLINGKYMISTSETIIDINGTVMNYSGWSHRDDFLHGMGYSATKEILIVQILATDPT
  KPLDVRYSFFVPKKSTPKVNSVTSHGSNKVGSHTSQPQWVTGPWLACSRTCDTGWHTRTVQCQDGNRKLA
  KGCPLSQRPSAFKQCLLKKC
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007038
• ORF Size: 2790 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007038.1, NM_007038.2, NM_007038.3, NM_007038.4, NP_008969.1
• RefSeq Size: 9663 bp
• RefSeq ORF: 2793 bp
• Locus ID: 11096
• Domains: tsp_1, Reprolysin, ACR
• Protein Families: Druggable Genome, Protease, Secreted Protein
• MW: 101.7 kDa
• Gene Summary: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme contains two C-terminal TS motifs and functions as an aggrecanase that cleaves aggrecan, a major proteoglycan of cartilage, and may mediate cartilage destruction in osteoarthritis. [provided by RefSeq, Feb 2016]