CARD6 (NM_032587) Human Tagged ORF Clone

CAT#: RC210848

CARD6 (Myc-DDK-tagged)-Human caspase recruitment domain family, member 6 (CARD6)

Product Images

Western blot validation of overexpression lysate (Cat# LY410018) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210848 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CARD6
• Synonyms: CINCIN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210848 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACCGAGAGTACTCCCTCAGAGATCATAGAAAGAGAAAGAAAAAAGTTGCTTGAAATCCTTCAAC
  ATGATCCTGATTCTATCTTAGACACGTTAACTTCTCGGAGGCTGATTTCTGAGGAAGAGTATGAGACTCT
  GGAGAATGTTACAGATCTCCTGAAGAAAAGTCGGAAGCTGTTAATTTTGGTACAGAAAAAGGGAGAGGCG
  ACCTGTCAGCATTTTCTCAAGTGTTTATTTAGTACTTTTCCACAGTCAGCTGCCATTTGCGGCTTAAGGC
  ATGAAGTTTTAAAACATGAGAATACAGTACCTCCTCAATCTATGGGGGCAAGCAGTAATTCAGAAGATGC
  TTTTTCTCCTGGAATAAAACAGCCTGAAGCCCCTGAGATCACAGTGTTCTTCAGTGAGAAGGAACACTTG
  GATTTGGAAACCTCTGAGTTTTTCAGGGACAAGAAAACTAGTTATAGGGAAACAGCTTTGTCTGCCAGGA
  AGAATGAGAAGGAATATGACACACCAGAAGTCACATTATCATATTCAGTTGAGAAAGTTGGATGTGAAGT
  TCCAGCAACTATTACATATATAAAAGATGGACAGAGATATGAGGAGCTAGATGATTCTTTATACTTAGGA
  AAAGAGGAATATCTAGGATCTGTTGACACCCCTGAAGATGCAGAAGCCACTGTGGAAGAGGAGGTTTATG
  ATGACCCAGAGCACGTTGGATATGATGGTGAAGAGGACTTCGAGAATTCAGAAACCACAGAGTTCTCTGG
  TGAAGAACCAAGTTATGAGGGATCAGAAACCAGCCTTTCATTGGAGGAGGAACAGGAGAAAAGTATAGAA
  GAAAGAAAAAAGGTGTTTAAAGATGTCCTGTTATGTTTGAACATGGATAGAAGCAGAAAGGTTCTGCCAG
  ATTTTGTTAAACAATTCTCCTTAGATCGAGGATGTAAGTGGACCCCTGAGAGTCCAGGAGACTTAGCCTG
  GAATTTCCTGATGAAAGTTCAAGCACGAGATGTGACGGCTAGGGATTCAATCCTCAGTCACAAGGTTCTG
  GATGAAGATAGCAAGGAGGATTTGCTGGCTGGAGTGGAGAATTTGGAAATTCGAGACATACAAACCATTA
  ATCCCCTTGACGTGCTTTGTGCCACCATGCTGTGTTCAGATAGCTCTTTGCAACGCCAAGTCATGTCAAA
  CATGTATCAGTGCCAGTTTGCTCTTCCCCTGCTACTGCCAGATGCAGAAAACAACAAAAGCATCTTAATG
  CTGGGGGCCATGAAAGACATTGTGAAGAAGCAGTCAACACAGTTTTCAGGGGGGCCTACAGAGGATACAG
  AAAAGTTTCTGACTCTCATGAAGATGCCTGTCATCTCTTTTGTGCGTCTAGGATACTGTAGCTTCTCTAA
  GTCCAGAATCCTCAACACACTTCTCAGCCCTGCCCAGTTGAAATTACACAAAATCTTTCTTCATCAAGAT
  TTGCCTCTTTTGGTGCTTCCCCGGCAAATCTCTGATGGCCTGGTTGAGATAACATGGTGTTTTCCTGATA
  GCGATGATAGAAAGGAAAACCCCTTTTTCCAAAAGCCTGTTGCTCTGGCTAATCTCCGTGGAAATCTAGA
  AAGCTTTTGGACTCAGTTTGGTTTTTTGATGGAAGTTTCTTCAGCTGTGTTTTTTTTCACTGACTGTTTA
  GGTGAGAAGGAATGGGACTTGCTAATGTTTTTAGGAGAGGCTGCCATTGAAAGATGCTACTTTGTTCTCA
  GTTCCCAAGCCAGGGAGAGTGAAGAGGCTCAAATTTTTCAGAGGATACTGAACTTGAAGCCAGCACAGCT
  ACTGTTTTGGGAGAGGGGAGATGCTGGGGATAGAAGGAAGAACATGGAGGGCCTTCAAGCTGCCCTCCAG
  GAAGTGATGTTCTCTTCTTGCCTCAGATGTGTGTCTGTGGAGGATATGGCCGCCCTGGCCAGGGAGCTGG
  GGATTCAGGTAGATGAAGACTTTGAAAACACTCAGAGAATTCAAGTTTCCTCTGGAGAAAACATGGCTGG
  GACAGCTGAAGGTGAGGGTCAGCAAAGACACAGTCAGCTAAAAAGCTCATCTAAAAGCCAGGCTCTAATG
  CCAATTCAAGAGCCTGGGACTCAATGTGAGCTCAGCCAGAATCTTCAGAATCTCTATGGTACCCCAGTAT
  TCAGGCCTGTTCTAGAGAACTCCTGGCTCTTTCCAACCAGAATTGGAGGTAACTTTAACCATGTTTCCTT
  GAAAGCCTCCTGGGTTATGGGCCGCCCCTTTGGGTCAGAGCAGAGGCCTAAGTGGTTCCATCCTTTGCCT
  TTTCAGAATGCAGGGGCCCAGGGCCGAGGTAAAAGTTTTGGTATTCAATCCTTCCATCCCCAGATATTTT
  ATTCAGGTGAAAGATTCATGAAATTTTCCAGAGTTGCTCGGGGATGTCACTCGAATGGAACATTTGGGAG
  ACTGCCAAGACCCATTTGTCAGCATGTACAGGCCTGCCCTGAGAGACCACAAATGATGGGAACTCTTGAA
  AGGTCTAGGGCAGTAGCCTCCAAGATAGGTCACTCCTATTCCCTGGATTCACAGCCAGCAAGAGCAGTAG
  GGAAGCCATGGCCTCAGCAAGCTTGCACCAGGGTAACAGAGTTAACTGAAGCAACTGGAAAACTGATAAG
  AACATCCCATATTGGAAAGCCTCACCCTCAGTCCTTTCAACCAGCAGCAGCCACACAAAAACTAAGACCT
  GCTTCTCAGCAAGGAGTCCAGATGAAGACACAAGGTGGGGCTTCAAATCCAGCTCTCCAAATAGGGTCCC
  ATCCCATGTGCAAGAGCTCTCAGTTCAAATCCGATCAGTCCAACCCATCCACAGTCAAACACTCCCAGCC
  TAAACCCTTCCATTCTGTGCCCTCTCAACCTAAATCCTCTCAGACAAAATCCTGTCAGTCCCAGCCCTCC
  CAAACTAAACCTTCTCCATGCAAATCTACTCAGCCTAAGCCAAGCCAGCCCTGGCCTCCCCAGTCTAAGC
  CTTCTCAGCCCAGACCCCCTCAACCTAAGTCATCCTCAACCAATCCTTCACAAGCTAAGGCACACCACTC
  AAAAGCAGGGCAGAAGAGGGGAGGGAAGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210848 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATESTPSEIIERERKKLLEILQHDPDSILDTLTSRRLISEEEYETLENVTDLLKKSRKLLILVQKKGEA
  TCQHFLKCLFSTFPQSAAICGLRHEVLKHENTVPPQSMGASSNSEDAFSPGIKQPEAPEITVFFSEKEHL
  DLETSEFFRDKKTSYRETALSARKNEKEYDTPEVTLSYSVEKVGCEVPATITYIKDGQRYEELDDSLYLG
  KEEYLGSVDTPEDAEATVEEEVYDDPEHVGYDGEEDFENSETTEFSGEEPSYEGSETSLSLEEEQEKSIE
  ERKKVFKDVLLCLNMDRSRKVLPDFVKQFSLDRGCKWTPESPGDLAWNFLMKVQARDVTARDSILSHKVL
  DEDSKEDLLAGVENLEIRDIQTINPLDVLCATMLCSDSSLQRQVMSNMYQCQFALPLLLPDAENNKSILM
  LGAMKDIVKKQSTQFSGGPTEDTEKFLTLMKMPVISFVRLGYCSFSKSRILNTLLSPAQLKLHKIFLHQD
  LPLLVLPRQISDGLVEITWCFPDSDDRKENPFFQKPVALANLRGNLESFWTQFGFLMEVSSAVFFFTDCL
  GEKEWDLLMFLGEAAIERCYFVLSSQARESEEAQIFQRILNLKPAQLLFWERGDAGDRRKNMEGLQAALQ
  EVMFSSCLRCVSVEDMAALARELGIQVDEDFENTQRIQVSSGENMAGTAEGEGQQRHSQLKSSSKSQALM
  PIQEPGTQCELSQNLQNLYGTPVFRPVLENSWLFPTRIGGNFNHVSLKASWVMGRPFGSEQRPKWFHPLP
  FQNAGAQGRGKSFGIQSFHPQIFYSGERFMKFSRVARGCHSNGTFGRLPRPICQHVQACPERPQMMGTLE
  RSRAVASKIGHSYSLDSQPARAVGKPWPQQACTRVTELTEATGKLIRTSHIGKPHPQSFQPAAATQKLRP
  ASQQGVQMKTQGGASNPALQIGSHPMCKSSQFKSDQSNPSTVKHSQPKPFHSVPSQPKSSQTKSCQSQPS
  QTKPSPCKSTQPKPSQPWPPQSKPSQPRPPQPKSSSTNPSQAKAHHSKAGQKRGGKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032587
• ORF Size: 3111 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032587.1, NM_032587.2, NM_032587.3, NP_115976.2
• RefSeq Size: 4097 bp
• RefSeq ORF: 3114 bp
• Locus ID: 84674
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• MW: 116.5 kDa
• Gene Summary: This gene encodes a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB. [provided by RefSeq, Jul 2008]