PCDHGA2 (NM_032009) Human Tagged ORF Clone

CAT#: RC210863

PCDHGA2 (Myc-DDK-tagged)-Human protocadherin gamma subfamily A, 2 (PCDHGA2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY410404) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210863 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PCDHGA2 protein (Cat# TP310863). The protein was produced from HEK293T cells transfected with PCDHGA2 cDNA clone (Cat# RC210863) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDHGA2
• Synonyms: PCDH-GAMMA-A2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210863 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTCTGCAAAAGTTGCCACACTGCAGAAAGCTGGTCCTGCTGTGCTTCCTTTTGGCGACCCTGT
  GGGAGGCCAGGGCCGGGCAGATTCGCTATTCTGTGCGGGAAGAGATCGACAGAGGCTCCTTCGTAGGCAA
  CATCGCCAAGGACTTGGGTTTGGAGCCCCTGGCACTGGCAGAGCAGGGAGTCCGCATCGTCTCCAGAGGT
  AGGTCCCAGCTCTTTGCTCTGAACCCGCGAAGCGGCAGCTTGGTCACTGCGAACAGGATAGACCGGGAGG
  AGCTCTGCGCTCAGAGCGCACCCTGTCTGTTGAATTTTAACATTCTGCTGGAGGATAAATTGACTATTTA
  TTCAGTAGAGGTGGAAATAACAGATATTAACGATAATGCCCCTCGCTTTGGAGTAGAGGAACTGGAGCTA
  AAAATCAGTGAAACCACTACGCCAGGATTCCGGATTCCTCTTAAGAATGCGCATGATGCAGACGTAGGTG
  AGAACGCCCTTCAGAAGTACGCACTCAACCCAAATGACCACTTCTCCCTGGACGTGCGAAGAGGAGCTGA
  TGGGAACAAGTACCCAGAACTGGTGCTGGAGCGCTCTCTGGACCGCGAGGAAGAGGCTGTTCACCACCTC
  GTTCTCGTGGCTTCTGATGGGGGTGACCCAGTGCTATCTGGCACCTCCCGCATCTGCGTGAAGGTCCTGG
  ATGCGAACGACAATGCGCCTGTTTTTACACAGCCCGAGTACCGCATAAGCATTCCGGAGAATACGCTCGT
  GGGCACCCGGATACTCACGGTGACCGCCACTGACGCAGATGAGGGCTACTACGCTCAAGTGGTATATTTT
  CTAGAGAAAAGCCCTGGAGAAACCTCAGAGGTATTTGAGCTTAAGTCAACATCTGGAGAACTGACAATCA
  TAAAAGATCTAGATTATGAGGATGCTACATTCCATGAAATTGATATTGAAGCTCAGGATGGTCCGGGCCT
  TCTAACCAGAGCGAAGGTTATCGTCACGGTTCTGGATGTGAATGACAATGCCCCAGAATTTTACATGACA
  TCTGCTACTAGCTCAGTTTCTGAAGACTCTCTTCCAGGAACCATAATTGGGCTTTTTAATGTACATGATA
  GAGACTCTGGGCAGAACGCATTCACCACCTGTTCACTCCCCGAGGATCTTCCTTTTAAGTTAGAAAAGTC
  AGTAGACAATTACTACCGACTGGTTACAACCAGAGCCCTTGACAGGGAACAGTTTTCCTTTTACAACATC
  ACTCTAACCGCTAAAGATGGAGGGAACCCCTCCCTGTCCACGGATGCTCACATTTTGCTCCAGGTGGCAG
  ACATCAACGACAACGCACCCGCCTTCTCCCGCACATCCTACTCCACCTACATTCCCGAAAACAACCCCAG
  AGGAGCCTCTGTCTTCTCAGTGACGGCCCATGACCCCGACAGCAACGACAATGCTCATGTAACTTACTCT
  TTCGCGGAGGACACTGTTCAGGGGGCACCCTTATCCTCTTACATCTCTATCAACTCCGACACTGGAGTAC
  TCTATGCACTGCGCTCCTTTGATTATGAGCAGTTGCGAGACTTGCAAGTGTGGGTGATAGCGCGGGACAG
  CGGGAACCCTCCACTCAGTAGCAATGTATCATTAAGCCTGTTCGTGCTGGACCAGAACGACAACGCGCCC
  GAGATCCTGTACCCTGCCTTCCCCACAGACGGTTCCACTGGCGTGGAGCTGGCGCCCCGCTCCGCAGAGC
  CCGGCTACCTGGTGACCAAGGTGGTGGCGGTGGACAGAGACTCGGGCCAGAACGCCTGGCTGTCTTACCA
  CCTGCTCAAGGCCAGCGAGCCGGGACTCTTCTCGGTGGGTCTGCACACGGGCGAGGTGCGCACGGCGCGA
  GCCCTGCTGGACAGAGACGCGCTCAAGCAGAGCCTCGTGGTGGCCATCCAGGACCACGGCCAGCCCCCTC
  TCTCCGCCACTGTCACGCTCACCGTGGCCGTGGCCGACAGGATCCCCGACATCCTGGCCGACCTGGGCAG
  CCTCGAGCCCTCCGCCATACCCAACGATTCGGACCTCACTCTGTACCTGGTGGTGGCGGTGGCCGCGGTC
  TCCTGCGTCTTCCTGGCCTTCGTCATCGTGTTGCTGGCGCACAGGCTGCGGCGCTGGCACAAGTCACGCC
  TGCTGCAGGCTTCAGGAGGCAGCTTGACAGGCATGCAGAGCTCGCACTTTGTGGGCGTGGACGGGGTTCG
  GGCTTTCCTGCAGACCTATTCCCACGAGGTCTCCCTCACTGCGGACTCGCGGAAGAGCCACCTGATTTTC
  CCCCAGCCCAACTATGCGGACACGCTCATCAGCCAGGAGAGCTGTGAGAAAAAGGATTTTTTATCAGCGC
  CTCAATCTCTACTCGAAGAAGAAAGAGAAGAAACGTTTTCTCAGGTAATCTATCTTTTCACAACATACGT
  ACTAGCTAGTTTGCTGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210863 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALQKLPHCRKLVLLCFLLATLWEARAGQIRYSVREEIDRGSFVGNIAKDLGLEPLALAEQGVRIVSRG
  RSQLFALNPRSGSLVTANRIDREELCAQSAPCLLNFNILLEDKLTIYSVEVEITDINDNAPRFGVEELEL
  KISETTTPGFRIPLKNAHDADVGENALQKYALNPNDHFSLDVRRGADGNKYPELVLERSLDREEEAVHHL
  VLVASDGGDPVLSGTSRICVKVLDANDNAPVFTQPEYRISIPENTLVGTRILTVTATDADEGYYAQVVYF
  LEKSPGETSEVFELKSTSGELTIIKDLDYEDATFHEIDIEAQDGPGLLTRAKVIVTVLDVNDNAPEFYMT
  SATSSVSEDSLPGTIIGLFNVHDRDSGQNAFTTCSLPEDLPFKLEKSVDNYYRLVTTRALDREQFSFYNI
  TLTAKDGGNPSLSTDAHILLQVADINDNAPAFSRTSYSTYIPENNPRGASVFSVTAHDPDSNDNAHVTYS
  FAEDTVQGAPLSSYISINSDTGVLYALRSFDYEQLRDLQVWVIARDSGNPPLSSNVSLSLFVLDQNDNAP
  EILYPAFPTDGSTGVELAPRSAEPGYLVTKVVAVDRDSGQNAWLSYHLLKASEPGLFSVGLHTGEVRTAR
  ALLDRDALKQSLVVAIQDHGQPPLSATVTLTVAVADRIPDILADLGSLEPSAIPNDSDLTLYLVVAVAAV
  SCVFLAFVIVLLAHRLRRWHKSRLLQASGGSLTGMQSSHFVGVDGVRAFLQTYSHEVSLTADSRKSHLIF
  PQPNYADTLISQESCEKKDFLSAPQSLLEEEREETFSQVIYLFTTYVLASLLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032009
• ORF Size: 2469 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032009.1, NM_032009.2, NP_114398.1
• RefSeq Size: 2735 bp
• RefSeq ORF: 2472 bp
• Locus ID: 56113
• Domains: CA
• Protein Families: Transmembrane
• MW: 90.3 kDa
• Gene Summary: This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq, Jul 2008]