SP7 (NM_152860) Human Tagged ORF Clone

CAT#: RC210939

SP7 (Myc-DDK-tagged)-Human Sp7 transcription factor (SP7), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY403500) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210939 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SP7
• Synonyms: OI11; OI12; osterix; OSX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210939 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCTCCCTGCTTGAGGAGGAAGTTCACTATGGCTCCAGTCCCCTGGCCATGCTGACGGCAGCGT
  GCAGCAAATTTGGTGGCTCTAGCCCTCTGCGGGACTCAACAACTCTGGGCAAAGCAGGCACAAAGAAGCC
  GTACTCTGTGGGCAGTGACCTTTCAGCCTCCAAAACCATGGGGGATGCTTATCCAGCCCCCTTTACAAGC
  ACTAATGGGCTCCTTTCACCTGCAGGCAGTCCTCCAGCACCCACCTCAGGCTATGCTAATGATTACCCTC
  CCTTTTCCCACTCATTCCCTGGGCCCACAGGCACCCAGGACCCTGGGCTACTAGTGCCCAAGGGGCACAG
  CTCTTCTGACTGTCTGCCCAGTGTCTACACCTCTCTGGACATGACACACCCCTATGGCTCCTGGTACAAG
  GCAGGCATCCATGCAGGCATTTCACCAGGCCCAGGCAACACTCCTACTCCATGGTGGGATATGCACCCTG
  GAGGCAACTGGCTAGGTGGTGGGCAGGGCCAGGGTGATGGGCTGCAAGGGACACTGCCCACAGGTCCAGC
  TCAGCCTCCACTGAACCCCCAGCTGCCCACCTACCCATCTGACTTTGCTCCCCTTAATCCAGCCCCCTAC
  CCAGCTCCCCACCTCTTGCAACCAGGGCCCCAGCATGTCTTGCCCCAAGATGTCTATAAACCCAAGGCAG
  TGGGAAATAGTGGGCAGCTAGAAGGGAGTGGTGGAGCCAAACCCCCACGGGGTGCAAGCACTGGGGGTAG
  TGGTGGATATGGGGGCAGTGGGGCAGGGCGCTCCTCCTGCGACTGCCCTAATTGCCAGGAGCTAGAGCGG
  CTGGGAGCAGCAGCGGCTGGGCTGCGGAAGAAGCCCATCCACAGCTGCCACATCCCTGGCTGCGGCAAGG
  TGTATGGCAAGGCTTCGCACCTGAAGGCCCACTTGCGCTGGCACACAGGCGAGAGGCCCTTCGTCTGCAA
  CTGGCTCTTCTGCGGCAAGAGGTTCACTCGTTCGGATGAGCTGGAGCGTCATGTGCGCACTCACACCCGG
  GAGAAGAAGTTCACCTGCCTGCTCTGCTCCAAGCGCTTTACCCGAAGTGACCACCTGAGCAAACACCAGC
  GCACCCACGGAGAACCAGGCCCGGGTCCCCCTCCCAGTGGCCCCAAGGAGCTGGGGGAGGGCCGCAGCAC
  GGGGGAAGAGGAGGCCAGTCAGACGCCCCGACCTTCTGCCTCGCCAGCAACCCCAGAGAAAGCCCCTGGA
  GGCAGCCCTGAGCAGAGCAACTTGCTGGAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210939 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASSLLEEEVHYGSSPLAMLTAACSKFGGSSPLRDSTTLGKAGTKKPYSVGSDLSASKTMGDAYPAPFTS
  TNGLLSPAGSPPAPTSGYANDYPPFSHSFPGPTGTQDPGLLVPKGHSSSDCLPSVYTSLDMTHPYGSWYK
  AGIHAGISPGPGNTPTPWWDMHPGGNWLGGGQGQGDGLQGTLPTGPAQPPLNPQLPTYPSDFAPLNPAPY
  PAPHLLQPGPQHVLPQDVYKPKAVGNSGQLEGSGGAKPPRGASTGGSGGYGGSGAGRSSCDCPNCQELER
  LGAAAAGLRKKPIHSCHIPGCGKVYGKASHLKAHLRWHTGERPFVCNWLFCGKRFTRSDELERHVRTHTR
  EKKFTCLLCSKRFTRSDHLSKHQRTHGEPGPGPPPSGPKELGEGRSTGEEEASQTPRPSASPATPEKAPG
  GSPEQSNLLEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152860
• ORF Size: 1293 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152860.1, NP_690599.1
• RefSeq Size: 2995 bp
• RefSeq ORF: 1296 bp
• Locus ID: 121340
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• MW: 45 kDa
• Gene Summary: This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation. [provided by RefSeq, Jul 2010]