VEPH1 (NM_024621) Human Tagged ORF Clone

CAT#: RC210974

VEPH1 (Myc-DDK-tagged)-Human ventricular zone expressed PH domain homolog 1 (zebrafish) (VEPH1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY403012) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210974 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VEPH1
• Synonyms: MELT; VEPH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210974 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCAACTGTTCAGACTGGTTTTGGGACAAAAAGATCTTTCACGAGCTGGGGACCTCTTCTCCTTAG
  ATGACTCTGAGATTGAAGACAGCCTTACAGAAGCTTTGGAGCAAATTAAGATAATTAGCTCATCTTCAGA
  TTACCAAACCAATAACAATGACCAGGCAGTAGTTGAAATCTGTATCACAAGAATCACAACAGCCATCAGA
  GAGACCGAGTCCATTGAAAAGCATGCAAAGGCCCTTGTGGGGCTCTGGGACTCCTGCTTGGAACATAACC
  TGAGACCCTTTGGGAAAGACGAAGACACTCCTCATGCAAAAATCGCATCTGATATCATGAGTTGCATTTT
  ACAGAATTACAACCGACCCCCAGTGATGGCATTAGCCATCCCCATTGCAGTGAAATTCCTCCACAGAGGC
  AACAAGGAACTGTGCAGGAATATGTCTAACTACCTGTCTCTGGCTGCAATTACCAAGGCAGATCTCCTGG
  CTGATCACACGGAAGTTATAGTAAAGAGCATACTCCAAGGTAACACCATGTTGTTGAGAGTGTTACCTGC
  TGTGTATGAAAAGCAGCCTCAGCCAATTAATAGACACCTGACAGAACTCCTGGCCTTGATGTCTCAGCTG
  GAACAGCCAGAACAGTACCATCTACTACGGCTTTTGCATGTAGCAGCAAAGAAAAAACAACTCGAGGTAG
  TTCAGAAGTGTATTCCTTTCCTAATTGGGCATTTGAAGGATTCAACCCATAATGACATCATCCTAAACAT
  CCTCATAGAGATAGCAGTCTATGAGCCAGTGGCTTTGAACAGTTTTCTTCCAATGCTGAAAGAGATTGGT
  GAGAGATTCCCCTACCTCACTGGACAGATGGCAAGGATTTATGGAGCTGTTGGGCATGTGGATGAAGAGA
  GAGCCAGGAGCTGCCTGACATACCTGGTGAGCCAACTGGCCAACATGGAGCATTCGTTTCACCATATTCT
  CCTGCTGGAGATTAAAAGCATCACCGACACCTTCTCCTCAATCTTGGGCCCTCAGAGCAGAGACATCTTC
  CGCATGAGCAACAGCTTCACCGCCATTGCTAAACTCCTTACCCGACAACTGGAAAATACCAAGGCTGGAA
  GTGGCAGGAGAAAAATCAGCACTGAAATTGAATTCCCTGAGAAACTGGAAGAAACCAAGCTCATAGTAAC
  TGAAAATGAAGACCATGAAAAACTCCAAGTTAAAATCCAGGCTTTTGAAGACAAGATAAATGCAGGGAGC
  AATACCCCTGGCTCTATCAGAAGATATAGTCTGGGCCAAGTTTCTAAAGAAGAAAGAAAAAACATTAGAT
  TTAACAGGTCAAAAAGTTTGGCTTTCCACACTATGCTCACAAAGGGTGTGGGTTCAGATGACGGCGAAGA
  TGAAAACAGGGGAGACATACCAGCCAGCATCTCTCTTTCAGAAATAGACCCACTTGGCCAAGGAAATGAC
  AAGCTGCCGTTTAAGACAGACACTGAGAGATCACAGCTGGGGGAGTCTTCAGTTTCATACCCAAATATTA
  TACATATAGACTCAGAGAATTTGCCAGAAACTGTTAAAGAAAACTCCCAGGAAGAAACTCCAGAGACAAC
  TGCAAGTCCTATAGAATACCAAGATAAGCTCTACTTGCACTTAAAAAAAAACCTCAGCAAAGTGAAAGCA
  TATGCCATGGAAATTGGAAAGAAGATTCCAGTCCCTGATCAGTGTACCATTGAAGACACTGTGAGAAGTT
  GTGTAGCAAAGTTGTTCTTCACCTGCTCCCTGAAGGGTCATTACTGCCTATACAGTAAGTCCAGTTTTAT
  TCTCATCAGCCAAGAACCTCAGCCATGGATCCAGATCATGTTTCTATTTCAGCAGAGCCTGTTTCCTGAA
  CCCCTGTCCATTCAGAGTCATTCTGTGCAATTCCTCAGAGCTCTGTGGGAGAAGACCCAGGCAGGGGGTG
  CTCACAGCTTTGAAACTGCCATGATGGAGTCCACGTTTCCACAGCAGAAGGATCTGGACCAGGTACAGCT
  CCATCTGGAAGAAGTGAGGTTCTTTGACGTGTTTGGCTTCAGTGAAACAGCAGGAGCATGGCAATGCTTC
  ATGTGCAACAATCCTGAGAAAGCAACTGTTGTAAATCAAGATGGCCAGCCTCTCATAGAAGGAAAGCTTA
  AAGAGAAGCAAGTCAGATGGAAGTTCATCAAAAGGTGGAAAACACGCTATTTTACACTGGCTGGAAATCA
  ACTTCTGTTTCAAAAAGGAAAGTCTAAAGATGACCCTGACGACTGCCCAATAGAACTCAGCAAAGTACAG
  AGTGTGAAGGCTGTGGCCAAGAAACGCAGGGACCGCTCTCTCCCCCGGGCTTTCGAAATCTTCACAGACA
  ATAAAACCTATGTCTTTAAGGCCAAGGATGAGAAGAATGCAGAAGAATGGCTCCAGTGCATCAACGTGGC
  AGTTGCCCAAGCCAAAGAAAGGGAAAGTAGAGAAGTAACCACATATCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210974 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHQLFRLVLGQKDLSRAGDLFSLDDSEIEDSLTEALEQIKIISSSSDYQTNNNDQAVVEICITRITTAIR
  ETESIEKHAKALVGLWDSCLEHNLRPFGKDEDTPHAKIASDIMSCILQNYNRPPVMALAIPIAVKFLHRG
  NKELCRNMSNYLSLAAITKADLLADHTEVIVKSILQGNTMLLRVLPAVYEKQPQPINRHLTELLALMSQL
  EQPEQYHLLRLLHVAAKKKQLEVVQKCIPFLIGHLKDSTHNDIILNILIEIAVYEPVALNSFLPMLKEIG
  ERFPYLTGQMARIYGAVGHVDEERARSCLTYLVSQLANMEHSFHHILLLEIKSITDTFSSILGPQSRDIF
  RMSNSFTAIAKLLTRQLENTKAGSGRRKISTEIEFPEKLEETKLIVTENEDHEKLQVKIQAFEDKINAGS
  NTPGSIRRYSLGQVSKEERKNIRFNRSKSLAFHTMLTKGVGSDDGEDENRGDIPASISLSEIDPLGQGND
  KLPFKTDTERSQLGESSVSYPNIIHIDSENLPETVKENSQEETPETTASPIEYQDKLYLHLKKNLSKVKA
  YAMEIGKKIPVPDQCTIEDTVRSCVAKLFFTCSLKGHYCLYSKSSFILISQEPQPWIQIMFLFQQSLFPE
  PLSIQSHSVQFLRALWEKTQAGGAHSFETAMMESTFPQQKDLDQVQLHLEEVRFFDVFGFSETAGAWQCF
  MCNNPEKATVVNQDGQPLIEGKLKEKQVRWKFIKRWKTRYFTLAGNQLLFQKGKSKDDPDDCPIELSKVQ
  SVKAVAKKRRDRSLPRAFEIFTDNKTYVFKAKDEKNAEEWLQCINVAVAQAKERESREVTTYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024621
• ORF Size: 2499 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024621.1, NM_024621.2, NP_078897.1
• RefSeq Size: 4313 bp
• RefSeq ORF: 2502 bp
• Locus ID: 79674
• Domains: PH
• MW: 94.8 kDa