SIGLEC10 (NM_033130) Human Tagged ORF Clone

CAT#: RC210995

SIGLEC10 (Myc-DDK-tagged)-Human sialic acid binding Ig-like lectin 10 (SIGLEC10), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY409681) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210995 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIGLEC10
• Synonyms: PRO940; SIGLEC-10; SLG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210995 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTACTGCCACTGCTGCTGTCCTCGCTGCTGGGCGGGTCCCAGGCTATGGATGGGAGATTCTGGATAC
  GAGTGCAGGAGTCAGTGATGGTGCCGGAGGGCCTGTGCATCTCTGTGCCCTGCTCTTTCTCCTACCCCCG
  ACAAGACTGGACAGGGTCTACCCCAGCTTATGGCTACTGGTTCAAAGCAGTGACTGAGACAACCAAGGGT
  GCTCCTGTGGCCACAAACCACCAGAGTCGAGAGGTGGAAATGAGCACCCGGGGCCGATTCCAGCTCACTG
  GGGATCCCGCCAAGGGGAACTGCTCCTTGGTGATCAGAGACGCGCAGATGCAGGATGAGTCACAGTACTT
  CTTTCGGGTGGAGAGAGGAAGCTATGTGAGATATAATTTCATGAACGATGGGTTCTTTCTAAAAGTAACA
  GCCCTGACTCAGAAGCCTGATGTCTACATCCCCGAGACCCTGGAGCCCGGGCAGCCGGTGACGGTCATCT
  GTGTGTTTAACTGGGCCTTTGAGGAATGTCCACCCCCTTCTTTCTCCTGGACGGGGGCTGCCCTCTCCTC
  CCAAGGAACCAAACCAACGACCTCCCACTTCTCAGTGCTCAGCTTCACGCCCAGACCCCAGGACCACAAC
  ACCGACCTCACCTGCCATGTGGACTTCTCCAGAAAGGGTGTGAGCGTACAGAGGACCGTCCGACTCCGTG
  TGGCCTATGCCCCCAGAGACCTTGTTATCAGCATTTCACGTGACAACACGCCAGCCCTGGAGCCCCAGCC
  CCAGGGAAATGTCCCATACCTGGAAGCCCAAAAAGGCCAGTTCCTGCGGCTCCTCTGTGCTGCTGACAGC
  CAGCCCCCTGCCACACTGAGCTGGGTCCTGCAGAACAGAGTCCTCTCCTCGTCCCATCCCTGGGGCCCTA
  GACCCCTGGGGCTGGAGCTGCCCGGGGTGAAGGCTGGGGATTCAGGGCGCTACACCTGCCGAGCGGAGAA
  CAGGCTTGGCTCCCAGCAGCGAGCCCTGGACCTCTCTGTGCAGTATCCTCCAGAGAACCTGAGAGTGATG
  GTTTCCCAAGCAAACAGGACAGTCCTGGAAAACCTTGGGAACGGCACGTCTCTCCCAGTACTGGAGGGCC
  AAAGCCTGTGCCTGGTCTGTGTCACACACAGCAGCCCCCCAGCCAGGCTGAGCTGGACCCAGAGGGGACA
  GGTTCTGAGCCCCTCCCAGCCCTCAGACCCCGGGGTCCTGGAGCTGCCTCGGGTTCAAGTGGAGCACGAA
  GGAGAGTTCACCTGCCACGCTCGGCACCCACTGGGCTCCCAGCACGTCTCTCTCAGCCTCTCCGTGCACT
  ACTCCCCGAAGCTGCTGGGCCCCTCCTGCTCCTGGGAGGCTGAGGGTCTGCACTGCAGCTGCTCCTCCCA
  GGCCAGCCCGGCCCCCTCTCTGCGCTGGTGGCTTGGGGAGGAGCTGCTGGAGGGGAACAGCAGCCAGGAC
  TCCTTCGAGGTCACCCCCAGCTCAGCCGGGCCCTGGGCCAACAGCTCCCTGAGCCTCCATGGAGGGCTCA
  GCTCCGGCCTCAGGCTCCGCTGTGAGGCCTGGAACGTCCATGGGGCCCAGAGTGGATCCATCCTGCAGCT
  GCCAGATAAGAAGGGACTCATCTCAACGGCATTCTCCAACGGAGCGTTTCTGGGAATCGGCATCACGGCT
  CTTCTTTTCCTCTGCCTGGCCCTGATCATCATGAAGATTCTACCGAAGAGACGGACTCAGACAGAAACCC
  CGAGGCCCAGGTTCTCCCGGCACAGCACGATCCTGGATTACATCAATGTGGTCCCGACGGCTGGCCCCCT
  GGCTCAGAAGCGGAATCAGAAAGCCACACCAAACAGTCCTCGGACCCCTCTTCCACCAGGTGCTCCCTCC
  CCAGAATCAAAGAAGAACCAGAAAAAGCAGTATCAGTTGCCCAGTTTCCCAGAACCCAAATCATCCACTC
  AAGCCCCAGAATCCCAGGAGAGCCAAGAGGAGCTCCATTATGCCACGCTCAACTTCCCAGGCGTCAGACC
  CAGGCCTGAGGCCCGGATGCCCAAGGGCACCCAGGCGGATTATGCAGAAGTCAAGTTCCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210995 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLLPLLLSSLLGGSQAMDGRFWIRVQESVMVPEGLCISVPCSFSYPRQDWTGSTPAYGYWFKAVTETTKG
  APVATNHQSREVEMSTRGRFQLTGDPAKGNCSLVIRDAQMQDESQYFFRVERGSYVRYNFMNDGFFLKVT
  ALTQKPDVYIPETLEPGQPVTVICVFNWAFEECPPPSFSWTGAALSSQGTKPTTSHFSVLSFTPRPQDHN
  TDLTCHVDFSRKGVSVQRTVRLRVAYAPRDLVISISRDNTPALEPQPQGNVPYLEAQKGQFLRLLCAADS
  QPPATLSWVLQNRVLSSSHPWGPRPLGLELPGVKAGDSGRYTCRAENRLGSQQRALDLSVQYPPENLRVM
  VSQANRTVLENLGNGTSLPVLEGQSLCLVCVTHSSPPARLSWTQRGQVLSPSQPSDPGVLELPRVQVEHE
  GEFTCHARHPLGSQHVSLSLSVHYSPKLLGPSCSWEAEGLHCSCSSQASPAPSLRWWLGEELLEGNSSQD
  SFEVTPSSAGPWANSSLSLHGGLSSGLRLRCEAWNVHGAQSGSILQLPDKKGLISTAFSNGAFLGIGITA
  LLFLCLALIIMKILPKRRTQTETPRPRFSRHSTILDYINVVPTAGPLAQKRNQKATPNSPRTPLPPGAPS
  PESKKNQKKQYQLPSFPEPKSSTQAPESQESQEELHYATLNFPGVRPRPEARMPKGTQADYAEVKFQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033130
• ORF Size: 2091 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033130.1, NM_033130.2, NM_033130.3, NM_033130.4, NP_149121.2
• RefSeq Size: 3400 bp
• RefSeq ORF: 2094 bp
• Locus ID: 89790
• Protein Families: Druggable Genome, Transmembrane
• MW: 76.6 kDa
• Gene Summary: SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6 (MIM 604405). [supplied by OMIM, Jul 2002]