FBP17 (FNBP1) (NM_015033) Human Tagged ORF Clone

CAT#: RC211011

FNBP1 (Myc-DDK-tagged)-Human formin binding protein 1 (FNBP1)

Product Images

Western blot validation of overexpression lysate (Cat# LY414836) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211011 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FNBP1 protein (Cat# TP311011). The protein was produced from HEK293T cells transfected with FNBP1 cDNA clone (Cat# RC211011) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FNBP1
• Synonyms: FBP17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211011 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGGGGCACCGAGCTCTGGGATCAGTTTGACAACTTAGAAAAACACACACAGTGGGGAATTGATA
  TTCTTGAGAAATATATCAAGTTTGTGAAAGAAAGGACAGAGATTGAACTCAGCTATGCAAAGCAACTCAG
  GAATCTTTCAAAGAAGTACCAACCTAAAAAGAACTCGAAGGAGGAAGAAGAATACAAGTATACGTCATGT
  AAAGCTTTCATTTCCAACCTGAACGAAATGAATGATTACGCAGGGCAGCATGAAGTTATCTCCGAGAACA
  TGGCATCACAGATCATTGTGGACTTGGCACGCTATGTTCAGGAACTGAAACAGGAGAGGAAATCAAACTT
  TCACGATGGCCGTAAAGCACAGCAGCACATCGAGACTTGCTGGAAGCAGCTTGAATCTAGTAAAAGGCGA
  TTTGAACGCGATTGCAAAGAGGCGGACAGGGCGCAGCAGTACTTTGAGAAAATGGACGCTGACATCAATG
  TCACAAAAGCGGATGTTGAAAAGGCCCGACAACAAGCTCAAATACGTCACCAAATGGCAGAGGACAGCAA
  AGCAGATTACTCATCCATTCTCCAGAAATTCAACCATGAGCAGCATGAATATTACCATACTCACATCCCC
  AACATCTTCCAGAAAATACAAGAGATGGAGGAAAGGAGGATTGTGAGAATGGGAGAGTCCATGAAGACAT
  ATGCAGAGGTTGATCGGCAGGTGATCCCAATCATTGGGAAGTGCCTGGATGGAATAGTAAAAGCAGCCGA
  ATCAATTGATCAGAAAAATGATTCACAGCTGGTAATAGAAGCTTATAAATCAGGGTTTGAGCCTCCTGGA
  GACATTGAATTTGAGGATTACACTCAGCCAATGAAGCGCACTGTGTCAGATAACAGCCTTTCAAATTCCA
  GAGGAGAAGGCAAACCAGACCTCAAATTTGGTGGCAAATCCAAAGGAAAGTTATGGCCGTTCATCAAAAA
  AAATAAGCTTATGTCCCTTTTAACATCCCCCCATCAGCCTCCCCCTCCCCCTCCTGCCTCTGCCTCACCC
  TCTGCTGTTCCCAACGGCCCCCAGTCTCCCAAGCAGCAAAAGGAACCCCTCTCCCACCGCTTCAACGAGT
  TCATGACCTCCAAACCCAAAATCCACTGCTTCAGGAGCCTAAAGCGTGGGCTTTCTCTCAAGCTGGGTGC
  AACACCGGAGGATTTCAGCAACCTCCCACCTGAACAAAGAAGGAAAAAGCTGCAGCAGAAAGTCGATGAG
  TTAAATAAAGAAATTCAGAAGGAGATGGATCAAAGAGATGCCATAACAAAAATGAAAGATGTCTACCTAA
  AGAATCCTCAGATGGGAGACCCAGCCAGTTTGGATCACAAATTAGCAGAAGTCAGCCAAAATATAGAGAA
  ACTGCGAGTAGAGACCCAGAAATTTGAGGCCTGGCTGGCTGAGGTTGAAGGCCGGCTCCCAGCACGCAGC
  GAGCAGGCGCGCCGGCAGAGCGGACTGTACGACAGCCAGAACCCACCCACAGTCAACAACTGCGCCCAGG
  ACCGTGAGAGCCCAGATGGCAGTTACACAGAGGAGCAGAGTCAGGAGAGTGAGATGAAGGTGCTGGCCAC
  GGATTTTGACGACGAGTTTGATGATGAGGAGCCCCTCCCTGCCATAGGGACGTGCAAAGCTCTCTACACA
  TTTGAAGGTCAGAATGAAGGAACGATTTCCGTAGTTGAAGGAGAAACATTGTATGTCATAGAGGAAGACA
  AAGGCGATGGCTGGACCCGCATTCGGAGAAATGAAGATGAAGAGGGTTATGTCCCCACTTCATATGTCGA
  AGTCTGTTTGGACAAAAATGCCAAAGATTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211011 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSWGTELWDQFDNLEKHTQWGIDILEKYIKFVKERTEIELSYAKQLRNLSKKYQPKKNSKEEEEYKYTSC
  KAFISNLNEMNDYAGQHEVISENMASQIIVDLARYVQELKQERKSNFHDGRKAQQHIETCWKQLESSKRR
  FERDCKEADRAQQYFEKMDADINVTKADVEKARQQAQIRHQMAEDSKADYSSILQKFNHEQHEYYHTHIP
  NIFQKIQEMEERRIVRMGESMKTYAEVDRQVIPIIGKCLDGIVKAAESIDQKNDSQLVIEAYKSGFEPPG
  DIEFEDYTQPMKRTVSDNSLSNSRGEGKPDLKFGGKSKGKLWPFIKKNKLMSLLTSPHQPPPPPPASASP
  SAVPNGPQSPKQQKEPLSHRFNEFMTSKPKIHCFRSLKRGLSLKLGATPEDFSNLPPEQRRKKLQQKVDE
  LNKEIQKEMDQRDAITKMKDVYLKNPQMGDPASLDHKLAEVSQNIEKLRVETQKFEAWLAEVEGRLPARS
  EQARRQSGLYDSQNPPTVNNCAQDRESPDGSYTEEQSQESEMKVLATDFDDEFDDEEPLPAIGTCKALYT
  FEGQNEGTISVVEGETLYVIEEDKGDGWTRIRRNEDEEGYVPTSYVEVCLDKNAKDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015033
• ORF Size: 1851 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015033.1, NM_015033.2, NP_055848.1
• RefSeq Size: 5457
• RefSeq ORF: 1854
• Locus ID: 23048
• MW: 71.3 kDa
• Gene Summary: The protein encoded by this gene is a member of the formin-binding-protein family. The protein contains an N-terminal Fer/Cdc42-interacting protein 4 (CIP4) homology (FCH) domain followed by a coiled-coil domain, a proline-rich motif, a second coiled-coil domain, a Rho family protein-binding domain (RBD), and a C-terminal SH3 domain. This protein binds sorting nexin 2 (SNX2), tankyrase (TNKS), and dynamin; an interaction between this protein and formin has not been demonstrated yet in human. [provided by RefSeq, Jul 2008]