CLSTN3 (NM_014718) Human Tagged ORF Clone

CAT#: RC211077

CLSTN3 (Myc-DDK-tagged)-Human calsyntenin 3 (CLSTN3)

Product Images

Western blot validation of overexpression lysate (Cat# LY415092) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211077 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CLSTN3
• Synonyms: alcbeta; CDHR14; CSTN3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211077 representing NM_014718
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTCCTGCTGCTGCCCCTTCTGCTGGCCTCTCTGCTCGCGTCCTGCTCCTGTAACAAAGCCAACA
  AGCACAAGCCATGGATTGAGGCAGAGTACCAGGGCATCGTCATGGAGAATGACAACACGGTCCTACTGAA
  TCCACCACTCTTTGCCTTGGACAAGGATGCCCCGCTGCGCTATGCAGGTGAGATCTGCGGCTTCCGGCTC
  CATGGGTCTGGGGTGCCCTTTGAGGCTGTGATCCTTGACAAGGCGACAGGAGAGGGGCTGATCCGGGCCA
  AGGAGCCTGTGGACTGCGAGGCCCAGAAGGAACACACCTTCACCATCCAGGCCTATGACTGTGGCGAGGG
  CCCCGACGGGGCCAACACCAAGAAGTCCCACAAGGCCACTGTGCATGTGCGGGTCAACGATGTGAACGAG
  TTTGCCCCAGTGTTTGTGGAACGGCTGTATCGTGCGGCTGTGACAGAGGGGAAGCTGTACGATCGCATCC
  TGCGGGTGGAAGCCATTGACGGTGACTGCTCCCCCCAGTACAGCCAGATCTGCTACTATGAGATTCTCAC
  ACCCAACACCCCTTTCCTCATTGACAATGACGGGAACATTGAGAACACAGAGAAGCTGCAGTACAGTGGT
  GAGAGGCTCTATAAGTTTACAGTGACAGCTTATGACTGTGGGAAGAAGCGGGCAGCAGATGATGCTGAGG
  TGGAGATTCAGGTGAAGCCCACCTGTAAACCCAGCTGGCAAGGCTGGAACAAAAGGATCGAATATGCACC
  AGGTGCTGGGAGCTTGGCTTTGTTCCCTGGTATCCGCCTGGAGACCTGTGATGAACCACTCTGGAACATT
  CAGGCCACCATAGAGCTGCAGACCAGCCATGTGGCCAAGGGCTGTGACCGTGACAACTACTCAGAGCGGG
  CGCTGCGGAAACTCTGTGGTGCTGCCACTGGGGAGGTGGATCTGTTGCCCATGCCTGGCCCCAATGCCAA
  CTGGACAGCAGGACTCTCGGTGCACTACAGCCAGGACAGCAGCCTGATCTACTGGTTCAATGGCACCCAG
  GCTGTGCAGGTGCCCCTGGGTGGCCCCAGTGGGCTGGGCTCTGGGCCCCAGGACAGCCTCAGTGACCACT
  TCACCCTGTCCTTCTGGATGAAGCATGGCGTAACTCCCAACAAGGGCAAGAAGGAAGAGGAAACCATCGT
  ATGTAACACTGTCCAGAATGAGGACGGCTTCTCTCACTACTCGCTGACTGTCCACGGCTGTAGGATTGCC
  TTCCTCTACTGGCCCCTGCTTGAGAGTGCCCGCCCAGTCAAGTTCCTCTGGAAGCTGGAGCAGGTCTGTG
  ATGATGAGTGGCACCACTACGCTCTGAACCTCGAGTTCCCCACAGTCACACTCTATACCGACGGCATCTC
  CTTCGACCCTGCCCTCATCCATGACAATGGCCTCATCCACCCACCCCGAAGGGAGCCTGCTCTCATGATT
  GGGGCCTGCTGGACTGAGGAGAAGAACAAAGAGAAGGAAAAGGGAGACAACAGTACAGACACCACCCAAG
  GAGACCCTTTGTCGATCCACCACTACTTCCATGGCTACCTGGCTGGTTTCAGCGTGCGCTCAGGTCGCCT
  GGAGAGCCGCGAGGTCATCGAGTGCCTCTATGCATGTCGGGAGGGGCTGGACTATAGGGATTTCGAGAGC
  CTGGGCAAAGGCATGAAGGTCCACGTGAACCCCTCACAGTCCCTGCTCACCCTGGAGGGGGATGATGTGG
  AGACCTTCAACCATGCCCTGCAGCATGTGGCTTACATGAACACTCTGCGCTTTGCCACGCCCGGCGTCAG
  GCCCCTGCGCCTCACCACTGCTGTCAAGTGCTTCAGCGAAGAGTCCTGCGTCTCCATCCCTGAAGTGGAG
  GGCTACGTGGTCGTCCTTCAGCCTGACGCCCCCCAGATCCTGCTGAGTGGCACTGCTCATTTTGCCCGCC
  CAGCTGTGGACTTTGAGGGAACCAACGGCGTCCCTTTGTTCCCTGATCTTCAAATCACCTGCTCCATTTC
  TCACCAGGTGGAGGCCAAAAAGGATGAGAGTTGGCAGGGCACAGTGACAGACACACGCATGTCGGATGAG
  ATTGTGCACAACCTGGATGGCTGTGAAATTTCTCTGGTGGGGGATGACCTGGATCCCGAGCGGGAAAGCC
  TGCTCCTGGACACAACCTCTCTGCAGCAGCGGGGGCTGGAGCTCACCAACACATCTGCCTACCTCACTAT
  TGCTGGGGTGGAGAGCATCACTGTGTATGAAGAGATCCTGAGGCAGGCTCGTTATCGGCTGCGACACGGA
  GCTGCCCTCTACACCAGGAAGTTCCGGCTTTCCTGCTCGGAAATGAATGGCCGTTACTCCAGCAATGAAT
  TCATCGTGGAGGTCAATGTCCTGCACAGCATGAACCGGGTTGCCCACCCCAGCCACGTGCTCAGCTCCCA
  GCAGTTCCTGCACCGTGGTCACCAGCCCCCGCCTGAGATGGCTGGACACAGCCTAGCCAGCTCCCACAGA
  AACTCCATGATACCCAGCGCCGCAACCCTCATCATTGTGGTGTGCGTGGGCTTCCTGGTGCTCATGGTCG
  TCCTGGGCCTGGTGCGCATCCATTCCCTTCACCGCCGCGTCTCAGGGGCCGGCGGGCCTCCAGGGGCCTC
  CAGTGACCCCAAGGACCCAGACCTCTTCTGGGATGACTCAGCTCTCACCATCATTGTGAACCCCATGGAG
  TCCTACCAGAATCGGCAGTCCTGTGTGACGGGGGCTGTTGGGGGCCAGCAGGAGGATGAGGACAGCAGTG
  ACTCGGAGGTGGCCGATTCCCCCAGCAGCGACGAGAGACGCATCATCGAGACCCCCCCACACCGCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211077 representing NM_014718
  Red=Cloning site Green=Tags(s)
  
  MTLLLLPLLLASLLASCSCNKANKHKPWIEAEYQGIVMENDNTVLLNPPLFALDKDAPLRYAGEICGFRL
  HGSGVPFEAVILDKATGEGLIRAKEPVDCEAQKEHTFTIQAYDCGEGPDGANTKKSHKATVHVRVNDVNE
  FAPVFVERLYRAAVTEGKLYDRILRVEAIDGDCSPQYSQICYYEILTPNTPFLIDNDGNIENTEKLQYSG
  ERLYKFTVTAYDCGKKRAADDAEVEIQVKPTCKPSWQGWNKRIEYAPGAGSLALFPGIRLETCDEPLWNI
  QATIELQTSHVAKGCDRDNYSERALRKLCGAATGEVDLLPMPGPNANWTAGLSVHYSQDSSLIYWFNGTQ
  AVQVPLGGPSGLGSGPQDSLSDHFTLSFWMKHGVTPNKGKKEEETIVCNTVQNEDGFSHYSLTVHGCRIA
  FLYWPLLESARPVKFLWKLEQVCDDEWHHYALNLEFPTVTLYTDGISFDPALIHDNGLIHPPRREPALMI
  GACWTEEKNKEKEKGDNSTDTTQGDPLSIHHYFHGYLAGFSVRSGRLESREVIECLYACREGLDYRDFES
  LGKGMKVHVNPSQSLLTLEGDDVETFNHALQHVAYMNTLRFATPGVRPLRLTTAVKCFSEESCVSIPEVE
  GYVVVLQPDAPQILLSGTAHFARPAVDFEGTNGVPLFPDLQITCSISHQVEAKKDESWQGTVTDTRMSDE
  IVHNLDGCEISLVGDDLDPERESLLLDTTSLQQRGLELTNTSAYLTIAGVESITVYEEILRQARYRLRHG
  AALYTRKFRLSCSEMNGRYSSNEFIVEVNVLHSMNRVAHPSHVLSSQQFLHRGHQPPPEMAGHSLASSHR
  NSMIPSAATLIIVVCVGFLVLMVVLGLVRIHSLHRRVSGAGGPPGASSDPKDPDLFWDDSALTIIVNPME
  SYQNRQSCVTGAVGGQQEDEDSSDSEVADSPSSDERRIIETPPHRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014718
• ORF Size: 2868 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_014718.2, NM_014718.3, NP_055533.2
• RefSeq Size: 4029
• RefSeq ORF: 2871
• Locus ID: 9746
• Domains: CA
• Protein Families: Transmembrane
• MW: 106.1 kDa