GPRC5C (NM_022036) Human Tagged ORF Clone

CAT#: RC211081

GPRC5C (Myc-DDK-tagged)-Human G protein-coupled receptor, family C, group 5, member C (GPRC5C), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402899) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211081 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPRC5C
• Synonyms: RAIG-3; RAIG3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211081 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGGGCGTGGCAGTCAACAGCAACAACCCACACGCCGGCAGGGCCAGAAACTCCCATCTCCCTCAC
  CAGCCGGAAAGTACGAGTCGGCTCAGCCTGGAGGGACCCAACCAGAGCCTGGCCTGGGAGCCAGGATGGC
  CATCCACAAAGCCTTGGTGATGTGCCTGGGACTGCCTCTCTTCCTGTTCCCAGGGGCCTGGGCCCAGGGC
  CATGTCCCACCCGGCTGCAGCCAAGGCCTCAACCCCCTGTACTACAACCTGTGTGACCGCTCTGGGGCGT
  GGGGCATCGTCCTGGAGGCCGTGGCTGGGGCGGGCATTGTCACCACGTTTGTGCTCACCATCATCCTGGT
  GGCCAGCCTCCCCTTTGTGCAGGACACCAAGAAACGGAGCCTGCTGGGGACCCAGGTATTCTTCCTTCTG
  GGGACCCTGGGCCTCTTCTGCCTCGTGTTTGCCTGTGTGGTGAAGCCCGACTTCTCCACCTGTGCCTCTC
  GGCGCTTCCTCTTTGGGGTTCTGTTCGCCATCTGCTTCTCTTGTCTGGCGGCTCACGTCTTTGCCCTCAA
  CTTCCTGGCCCGGAAGAACCACGGGCCCCGGGGCTGGGTGATCTTCACTGTGGCTCTGCTGCTGACCCTG
  GTAGAGGTCATCATCAATACAGAGTGGCTGATCATCACCCTGGTTCGGGGCAGTGGCGAGGGCGGCCCTC
  AGGGCAACAGCAGCGCAGGCTGGGCCGTGGCCTCCCCCTGTGCCATCGCCAACATGGACTTTGTCATGGC
  ACTCATCTACGTCATGCTGCTGCTGCTGGGTGCCTTCCTGGGGGCCTGGCCCGCCCTGTGTGGCCGCTAC
  AAGCGCTGGCGTAAGCATGGGGTCTTTGTGCTCCTCACCACAGCCACCTCCGTTGCCATATGGGTGGTGT
  GGATCGTCATGTATACTTACGGCAACAAGCAGCACAACAGTCCCACCTGGGATGACCCCACGCTGGCCAT
  CGCCCTCGCCGCCAATGCCTGGGCCTTCGTCCTCTTCTACGTCATCCCCGAGGTCTCCCAGGTGACCAAG
  TCCAGCCCAGAGCAAAGCTACCAGGGGGACATGTACCCCACCCGGGGCGTGGGCTATGAGACCATCCTGA
  AAGAGCAGAAGGGTCAGAGCATGTTCGTGGAGAACAAGGCCTTTTCCATGGATGAGCCGGTTGCAGCTAA
  GAGGCCGGTGTCACCATACAGCGGGTACAATGGGCAGCTGCTGACCAGTGTGTACCAGCCCACTGAGATG
  GCCCTGATGCACAAAGTTCCGTCCGAAGGAGCTTACGACATCATCCTCCCACGGGCCACCGCCAACAGCC
  AGGTGATGGGCAGTGCCAACTCGACCCTGCGGGCTGAAGACATGTACTCGGCCCAGAGCCACCAGGCGGC
  CACACCGCCGAAAGACGGCAAGAACTCTCAGGTCTTTAGAAACCCCTACGTGTGGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211081 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRGRGSQQQQPTRRQGQKLPSPSPAGKYESAQPGGTQPEPGLGARMAIHKALVMCLGLPLFLFPGAWAQG
  HVPPGCSQGLNPLYYNLCDRSGAWGIVLEAVAGAGIVTTFVLTIILVASLPFVQDTKKRSLLGTQVFFLL
  GTLGLFCLVFACVVKPDFSTCASRRFLFGVLFAICFSCLAAHVFALNFLARKNHGPRGWVIFTVALLLTL
  VEVIINTEWLIITLVRGSGEGGPQGNSSAGWAVASPCAIANMDFVMALIYVMLLLLGAFLGAWPALCGRY
  KRWRKHGVFVLLTTATSVAIWVVWIVMYTYGNKQHNSPTWDDPTLAIALAANAWAFVLFYVIPEVSQVTK
  SSPEQSYQGDMYPTRGVGYETILKEQKGQSMFVENKAFSMDEPVAAKRPVSPYSGYNGQLLTSVYQPTEM
  ALMHKVPSEGAYDIILPRATANSQVMGSANSTLRAEDMYSAQSHQAATPPKDGKNSQVFRNPYVWD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022036
• ORF Size: 1458 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022036.2, NP_071319.2
• RefSeq Size: 2389 bp
• RefSeq ORF: 1326 bp
• Locus ID: 55890
• Domains: 7tm_3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 52.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the type 3 G protein-coupled receptor family. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The specific function of this protein is unknown; however, this protein may mediate the cellular effects of retinoic acid on the G protein signal transduction cascade. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]