HMG2L1 (HMGXB4) (NM_005487) Human Tagged ORF Clone

CAT#: RC211137

HMGXB4 (Myc-DDK-tagged)-Human HMG box domain containing 4 (HMGXB4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY415406) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215726 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified HMGXB4 protein (Cat# TP311137). The protein was produced from HEK293T cells transfected with HMGXB4 cDNA clone (Cat# RC211137) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HMGXB4
• Synonyms: high-mobility group protein 2-like 1; HMG2L1; HMGBCG; HMG box domain containing 4; OTTHUMP00000028778; THC211630
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211137 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTGTTGAAAGCTATCACTTCCCCACTGGCAGCAGGCTCCAAGCCCTCCAAAAAGACTGGGGAGA
  AATCCTCTGGCTCTTCAAGCCATTCGGAGAGTAAAAAGGAGCACCACAGGAAGAAAGTCAGTGGAAGCAG
  TGGGGAACTACCCCTAGAGGATGGTGTCTCCCACAAATCGAAAAAAATGAAACCTCTCTATGTGAACACA
  GAGACACTGACCCTTCGGGAGCCTGATGGTTTAAAAATGAAACTTATTCTGTCACCAAAGGAGAAGGGAA
  GCAGCTCTGTTGATGAGGAGTCTTTTCAATATCCCTCCCAACAAGCGACTGTGAAAAAATCCTCAAAGAA
  ATCAGCTCGGGATGAGCAGGGTGCTTTACTCCTAGGACATGAGTTACAGAGCTTTCTGAAAACAGCCCGG
  AAAAAGCACAAGTCATCCTCAGACGCACATTCATCTCCTGGCCCTGAAGGCTGTGGGTCTGACGCCTCCC
  AGTTCGCAGAGTCCCACAGTGCTAACCTTGATCTTTCAGGGCTTGAACCTATTCTGGTAGAATCAGACTC
  ATCCTCTGGTGGGGAACTAGAGGCTGGGGAGTTAGTGATAGATGATTCTTACCGAGAAATCAAGAAGAAA
  AAGAAGTCAAAGAAGAGCAAAAAGAAGAAAGACAAGGAGAAGCATAAAGAGAAGCGACACTCCAAGTCCA
  AGAGAAGTTTAGGACTTTCTGCCGTGCCAGTGGGAGAGGTCACAGTGACATCTGGCCCTCCTCCCAGCAT
  CCCATACGCTGGAGCAGCAGCACCTCCCCTGCCACTTCCTGGCCTCCACACAGATGGGCATAGTGAAAAA
  AAAAAGAAAAAAGAAGAGAAGGACAAAGAGAGAGAGAGAGGAGAAAAGCCAAAAAAGAAGAACATGTCGG
  CCTACCAGGTGTTCTGTAAAGAGTATCGCGTGACCATTGTGGCTGACCATCCAGGTATAGATTTTGGGGA
  ACTTAGTAAAAAACTGGCTGAGGTGTGGAAGCAATTACCAGAAAAAGACAAACTGATTTGGAAGCAAAAA
  GCTCAGTATCTGCAGCACAAACAGAACAAAGCAGAAGCCACAACTGTGAAAAGGAAAGCATCCAGCTCAG
  AAGGTTCCATGAAAGTCAAAGCCTCTTCTGTAGGAGTACTGTCACCCCAGAAGAAGTCCCCACCCACCAC
  CATGCTGTTACCAGCCTCACCAGCCAAAGCCCCTGAGACAGAGCCCATTGATGTTGCTGCTCATCTTCAG
  CTGTTGGGAGAGTCCCTAAGCCTCATTGGACACCGTCTGCAGGAAACTGAGGGTATGGTGGCTGTGTCTG
  GCAGTTTGTCAGTGCTTCTGGATTCCATTATCTGTGCCCTTGGCCCCTTGGCATGTCTCACCACACAACT
  ACCTGAATTGAATGGCTGTCCCAAACAGGTCTTGTCAAACACATTAGACAACATTGCTTACATCATGCCG
  GGACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211137 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLLKAITSPLAAGSKPSKKTGEKSSGSSSHSESKKEHHRKKVSGSSGELPLEDGVSHKSKKMKPLYVNT
  ETLTLREPDGLKMKLILSPKEKGSSSVDEESFQYPSQQATVKKSSKKSARDEQGALLLGHELQSFLKTAR
  KKHKSSSDAHSSPGPEGCGSDASQFAESHSANLDLSGLEPILVESDSSSGGELEAGELVIDDSYREIKKK
  KKSKKSKKKKDKEKHKEKRHSKSKRSLGLSAVPVGEVTVTSGPPPSIPYAGAAAPPLPLPGLHTDGHSEK
  KKKKEEKDKERERGEKPKKKNMSAYQVFCKEYRVTIVADHPGIDFGELSKKLAEVWKQLPEKDKLIWKQK
  AQYLQHKQNKAEATTVKRKASSSEGSMKVKASSVGVLSPQKKSPPTTMLLPASPAKAPETEPIDVAAHLQ
  LLGESLSLIGHRLQETEGMVAVSGSLSVLLDSIICALGPLACLTTQLPELNGCPKQVLSNTLDNIAYIMP
  GL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005487
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005487.2, NM_005487.3, NP_005478.2
• RefSeq Size: 4220 bp
• RefSeq ORF: 1478 bp
• Locus ID: 10042
• Protein Families: Transcription Factors
• MW: 53.2 kDa
• Gene Summary: High mobility group (HMG) proteins are nonhistone chromosomal proteins. See HMG2 (MIM 163906) for additional information on HMG proteins. [supplied by OMIM, Nov 2010]