G6PC2 (NM_021176) Human Tagged ORF Clone
CAT#: RC211146
G6PC2 (Myc-DDK-tagged)-Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1
"NM_021176" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | G6PC2 |
Synonyms | IGRP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC211146 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTCCTTCACAGGAATGGAGTGCTCATAATTCAGCATTTGCAGAAGGACTACCGAGCTTACTACA CTTTTCTAAATTTTATGTCCAATGTTGGAGACCCCAGGAATATCTTTTTCATTTATTTTCCACTTTGTTT TCAATTTAATCAGACAGTTGGAACCAAGATGATATGGGTAGCAGTCATTGGGGATTGGTTAAATCTTATA TTTAAATGGATATTATTTGGTCATCGACCTTACTGGTGGGTCCAAGAAACTCAGATTTACCCAAATCACT CAAGTCCATGCCTTGAACAGTTCCCTACTACATGTGAAACAGGTCCAGGAAGTCCATCTGGCCATGCAAT GGGCGCATCCTGTGTCTGGTATGTCATGGTAACCGCTGCCCTGAGCCACACTGTCTGTGGGATGGATAAG TTCTCTATCACTCTGCACAGACTGACCTGGTCATTTCTTTGGAGTGTTTTTTGGTTGATTCAAATCAGTG TCTGCATCTCCAGAGTATTCATAGCAACACATTTTCCTCATCAAGTTATTCTTGGAGTAATTGGTGGCAT GCTGGTGGCAGAGGCCTTTGAACACACTCCAGGCATCCAAACGGCCAGTCTGGGCACATACCTGAAGACC AACCTCTTTCTCTTCCTGTTTGCAGTTGGCTTTTACCTGCTTCTTAGGGTGCTCAACATTGACCTGCTGT GGTCCGTGCCCATAGCCAAAAAGTGGTGTGCTAACCCCGACTGGATCCACATTGACACCACGCCTTTTGC TGGACTCGTGAGAAACCTTGGGGTCCTCTTTGGCTTGGGCTTTGCAATCAACTCAGAGATGTTCCTCCTG AGCTGCCGAGGGGGAAATAACTACACACTGAGCTTCCGGTTGCTCTGTGCCTTGACCTCATTGACAATAC TGCAGCTCTACCATTTCCTCCAGATCCCGACTCACGAAGAGCATTTATTTTATGTGCTGTCTTTTTGTAA AAGTGCATCCATTCCCCTAACTGTGGTTGCTTTCATTCCCTACTCTGTTCATATGTTAATGAAACAAAGC GGAAAGAAGAGTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211146 protein sequence
Red=Cloning site Green=Tags(s) MDFLHRNGVLIIQHLQKDYRAYYTFLNFMSNVGDPRNIFFIYFPLCFQFNQTVGTKMIWVAVIGDWLNLI FKWILFGHRPYWWVQETQIYPNHSSPCLEQFPTTCETGPGSPSGHAMGASCVWYVMVTAALSHTVCGMDK FSITLHRLTWSFLWSVFWLIQISVCISRVFIATHFPHQVILGVIGGMLVAEAFEHTPGIQTASLGTYLKT NLFLFLFAVGFYLLLRVLNIDLLWSVPIAKKWCANPDWIHIDTTPFAGLVRNLGVLFGLGFAINSEMFLL SCRGGNNYTLSFRLLCALTSLTILQLYHFLQIPTHEEHLFYVLSFCKSASIPLTVVAFIPYSVHMLMKQS GKKSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021176 |
ORF Size | 1065 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021176.1, NM_021176.2, NP_066999.1 |
RefSeq Size | 3096 bp |
RefSeq ORF | 1068 bp |
Locus ID | 57818 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Adipocytokine signaling pathway, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, Starch and sucrose metabolism |
MW | 40.6 kDa |
Gene Summary | This gene encodes an enzyme belonging to the glucose-6-phosphatase catalytic subunit family. These enzymes are part of a multicomponent integral membrane system that catalyzes the hydrolysis of glucose-6-phosphate, the terminal step in gluconeogenic and glycogenolytic pathways, allowing the release of glucose into the bloodstream. The family member encoded by this gene is found in pancreatic islets and does not exhibit phosphohydrolase activity, but it is a major target of cell-mediated autoimmunity in diabetes. Several alternatively spliced transcript variants of this gene have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC304916 | G6PC2 (untagged)-Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1 |
USD 760.00 |
|
RG211146 | G6PC2 (GFP-tagged) - Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1 |
USD 460.00 |
|
RC211146L1 | Lenti ORF clone of Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC211146L2 | Lenti ORF clone of Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC211146L3 | Lenti ORF clone of Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC211146L4 | Lenti ORF clone of Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1, mGFP tagged |
USD 620.00 |
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