G6PC2 (NM_021176) Human Tagged ORF Clone

CAT#: RC211146

G6PC2 (Myc-DDK-tagged)-Human glucose-6-phosphatase, catalytic, 2 (G6PC2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412044) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211146 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: G6PC2
• Synonyms: IGRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211146 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTCCTTCACAGGAATGGAGTGCTCATAATTCAGCATTTGCAGAAGGACTACCGAGCTTACTACA
  CTTTTCTAAATTTTATGTCCAATGTTGGAGACCCCAGGAATATCTTTTTCATTTATTTTCCACTTTGTTT
  TCAATTTAATCAGACAGTTGGAACCAAGATGATATGGGTAGCAGTCATTGGGGATTGGTTAAATCTTATA
  TTTAAATGGATATTATTTGGTCATCGACCTTACTGGTGGGTCCAAGAAACTCAGATTTACCCAAATCACT
  CAAGTCCATGCCTTGAACAGTTCCCTACTACATGTGAAACAGGTCCAGGAAGTCCATCTGGCCATGCAAT
  GGGCGCATCCTGTGTCTGGTATGTCATGGTAACCGCTGCCCTGAGCCACACTGTCTGTGGGATGGATAAG
  TTCTCTATCACTCTGCACAGACTGACCTGGTCATTTCTTTGGAGTGTTTTTTGGTTGATTCAAATCAGTG
  TCTGCATCTCCAGAGTATTCATAGCAACACATTTTCCTCATCAAGTTATTCTTGGAGTAATTGGTGGCAT
  GCTGGTGGCAGAGGCCTTTGAACACACTCCAGGCATCCAAACGGCCAGTCTGGGCACATACCTGAAGACC
  AACCTCTTTCTCTTCCTGTTTGCAGTTGGCTTTTACCTGCTTCTTAGGGTGCTCAACATTGACCTGCTGT
  GGTCCGTGCCCATAGCCAAAAAGTGGTGTGCTAACCCCGACTGGATCCACATTGACACCACGCCTTTTGC
  TGGACTCGTGAGAAACCTTGGGGTCCTCTTTGGCTTGGGCTTTGCAATCAACTCAGAGATGTTCCTCCTG
  AGCTGCCGAGGGGGAAATAACTACACACTGAGCTTCCGGTTGCTCTGTGCCTTGACCTCATTGACAATAC
  TGCAGCTCTACCATTTCCTCCAGATCCCGACTCACGAAGAGCATTTATTTTATGTGCTGTCTTTTTGTAA
  AAGTGCATCCATTCCCCTAACTGTGGTTGCTTTCATTCCCTACTCTGTTCATATGTTAATGAAACAAAGC
  GGAAAGAAGAGTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211146 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDFLHRNGVLIIQHLQKDYRAYYTFLNFMSNVGDPRNIFFIYFPLCFQFNQTVGTKMIWVAVIGDWLNLI
  FKWILFGHRPYWWVQETQIYPNHSSPCLEQFPTTCETGPGSPSGHAMGASCVWYVMVTAALSHTVCGMDK
  FSITLHRLTWSFLWSVFWLIQISVCISRVFIATHFPHQVILGVIGGMLVAEAFEHTPGIQTASLGTYLKT
  NLFLFLFAVGFYLLLRVLNIDLLWSVPIAKKWCANPDWIHIDTTPFAGLVRNLGVLFGLGFAINSEMFLL
  SCRGGNNYTLSFRLLCALTSLTILQLYHFLQIPTHEEHLFYVLSFCKSASIPLTVVAFIPYSVHMLMKQS
  GKKSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021176
• ORF Size: 1065 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021176.1, NM_021176.2, NP_066999.1
• RefSeq Size: 3096 bp
• RefSeq ORF: 1068 bp
• Locus ID: 57818
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, Starch and sucrose metabolism
• MW: 40.6 kDa
• Gene Summary: This gene encodes an enzyme belonging to the glucose-6-phosphatase catalytic subunit family. These enzymes are part of a multicomponent integral membrane system that catalyzes the hydrolysis of glucose-6-phosphate, the terminal step in gluconeogenic and glycogenolytic pathways, allowing the release of glucose into the bloodstream. The family member encoded by this gene is found in pancreatic islets and does not exhibit phosphohydrolase activity, but it is a major target of cell-mediated autoimmunity in diabetes. Several alternatively spliced transcript variants of this gene have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]