POU4F3 (NM_002700) Human Tagged ORF Clone

CAT#: RC211206

POU4F3 (Myc-DDK-tagged)-Human POU class 4 homeobox 3 (POU4F3)

Product Images

Western blot validation of overexpression lysate (Cat# LY400949) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211206 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POU4F3
• Synonyms: BRN3C; DFNA15; DFNA42; DFNA52
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211206 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCCATGAACTCCAAGCAGCCTTTCGGCATGCACCCGGTGCTGCAAGAACCCAAATTCTCCAGTC
  TGCACTCTGGCTCCGAGGCTATGCGCCGAGTCTGTCTCCCAGCCCCGCAGCTGCAGGGTAATATATTTGG
  AAGCTTTGATGAGAGCCTGCTGGCACGCGCCGAAGCTCTGGCGGCGGTGGATATCGTCTCCCACGGCAAG
  AACCATCCGTTCAAGCCCGACGCCACCTACCATACCATGAGCAGCGTGCCCTGCACGTCCACTTCGTCCA
  CCGTGCCCATCTCCCACCCAGCTGCGCTCACCTCACACCCTCACCACGCCGTGCACCAGGGCCTCGAAGG
  CGACCTGCTGGAGCACATCTCGCCCACGCTGAGTGTGAGCGGCCTGGGCGCTCCGGAACACTCGGTGATG
  CCCGCACAGATCCATCCACACCACCTGGGCGCCATGGGCCACCTGCACCAGGCCATGGGCATGAGTCACC
  CGCACACCGTGGCCCCTCATAGCGCCATGCCTGCATGCCTCAGCGACGTGGAGTCAGACCCGCGCGAGCT
  GGAAGCCTTCGCCGAGCGCTTCAAGCAGCGGCGCATCAAGCTGGGGGTGACCCAGGCGGACGTGGGCGCG
  GCTCTGGCTAATCTCAAGATCCCCGGCGTGGGCTCGCTGAGCCAAAGCACCATCTGCAGGTTCGAGTCTC
  TCACTCTCTCGCACAACAACATGATCGCTCTCAAGCCGGTGCTCCAGGCCTGGTTGGAGGAGGCCGAGGC
  CGCCTACCGAGAGAAGAACAGCAAGCCAGAGCTCTTCAACGGCAGCGAACGGAAGCGCAAACGCACGTCC
  ATCGCGGCGCCGGAGAAGCGTTCACTCGAGGCCTATTTCGCTATCCAGCCACGTCCTTCATCTGAGAAGA
  TCGCGGCCATCGCTGAGAAACTGGACCTTAAAAAGAACGTGGTGAGAGTCTGGTTCTGCAACCAGAGACA
  GAAACAGAAACGAATGAAGTATTCGGCTGTCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211206 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMAMNSKQPFGMHPVLQEPKFSSLHSGSEAMRRVCLPAPQLQGNIFGSFDESLLARAEALAAVDIVSHGK
  NHPFKPDATYHTMSSVPCTSTSSTVPISHPAALTSHPHHAVHQGLEGDLLEHISPTLSVSGLGAPEHSVM
  PAQIHPHHLGAMGHLHQAMGMSHPHTVAPHSAMPACLSDVESDPRELEAFAERFKQRRIKLGVTQADVGA
  ALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPVLQAWLEEAEAAYREKNSKPELFNGSERKRKRTS
  IAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAVH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002700
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002700.1, NM_002700.2, NP_002691.1
• RefSeq Size: 1182 bp
• RefSeq ORF: 1017 bp
• Locus ID: 5459
• Cytogenetics: 5q32
• Protein Families: Transcription Factors
• MW: 37.1 kDa
• Gene Summary: 'This gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15. [provided by RefSeq, Mar 2009]'