POU4F3 (NM_002700) Human Tagged ORF Clone

CAT#: RC211206L4

Lenti ORF clone of Human POU class 4 homeobox 3 (POU4F3), mGFP tagged

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: POU4F3
• Synonyms: BRN3C; DFNA15; DFNA42; DFNA52
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC211206).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_002700
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002700.1, NP_002691.1
• RefSeq Size: 1182 bp
• RefSeq ORF: 1017 bp
• Locus ID: 5459
• Cytogenetics: 5q32
• Protein Families: Transcription Factors
• MW: 37.1 kDa
• Gene Summary: 'This gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15. [provided by RefSeq, Mar 2009]'