DGKB (NM_145695) Human Tagged ORF Clone

CAT#: RC211233

DGKB (Myc-DDK-tagged)-Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY407898) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211233 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DGKB
• Synonyms: DAGK2; DGK; DGK-BETA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211233 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAACCAGGAAAAATGGGCCCACCTCAGCCCTTCGGAATTTTCCCAACTTCAGAAATATGCTGAGT
  ATTCTACAAAGAAATTAAAGGATGTTCTTGAAGAATTCCATGGTAATGGTGTGCTTGCAAAGTATAATCC
  TGAAGGGAAACAAGACATTCTTAACCAAACAATAGATTTTGAAGGTTTCAAACTATTCATGAAGACATTC
  CTGGAAGCCGAGCTTCCTGATGATTTCACTGCACACCTTTTCATGTCATTTAGCAACAAGTTTCCTCATT
  CTAGTCCAATGGTAAAAAGTAAGCCTGCTCTCCTATCAGGCGGTCTGAGAATGAATAAAGGTGCCATCAC
  CCCTCCCCGGACTACTTCTCCTGCAAATACGTGTTCCCCAGAAGTAATCCATCTGAAGGACATTGTCTGT
  TACCTGTCTCTGCTTGAAAGAGGAAGACCTGAGGATAAGCTTGAGTTTATGTTTCGCCTTTATGACACGG
  ATGGGAATGGCTTCCTGGACAGCTCGGAGCTAGAAAATATCATCAGTCAGATGATGCATGTTGCAGAATA
  CCTTGAGTGGGATGTCACTGAACTTAATCCAATCCTCCATGAAATGATGGAAGAAATTGACTATGATCAT
  GATGGAACCGTGTCTCTGGAGGAATGGATTCAAGGAGGAATGACAACGATTCCACTTCTTGTGCTCCTGG
  GCTTAGAAAATAACGTGAAGGATGATGGACAGCACGTGTGGCGACTGAAGCACTTTAACAAACCTGCCTA
  TTGCAACCTTTGCCTGAACATGCTGATTGGCGTGGGGAAGCAGGGCCTCTGCTGTTCCTTCTGCAAGTAC
  ACAGTCCATGAGCGCTGTGTGGCTCGAGCACCTCCCTCTTGCATCAAGACCTATGTGAAGTCCAAAAGGA
  ACACTGATGTCATGCACCATTACTGGGTTGAAGGTAACTGCCCAACCAAGTGTGATAAGTGCCACAAAAC
  TGTTAAATGTTACCAGGGCCTGACAGGACTGCATTGTGTTTGGTGTCAGATCACACTGCATAATAAATGT
  GCTTCTCATCTAAAACCTGAATGTGACTGTGGACCTTTGAAGGACCATATTTTACCACCCACAACAATCT
  GTCCAGTGGTACTGCAGACTCTGCCCACTTCAGGAGTTTCAGTTCCTGAGGAAAGACAATCAACAGTGAA
  AAAGGAAAAGAGTGGTTCCCAGCAGCCAAACAAAGTGATTGACAAGAATAAAATGCAAAGAGCCAACTCT
  GTTACTGTAGATGGACAAGGCCTGCAGGTCACTCCTGTGCCTGGTACTCACCCACTTTTAGTTTTTGTGA
  ACCCCAAAAGTGGTGGAAAACAAGGAGAACGAATTTACAGAAAATTCCAGTATCTATTAAATCCTCGTCA
  GGTTTACAGTCTTTCTGGAAATGGACCAATGCCAGGGTTAAACTTTTTCCGTGATGTTCCTGACTTCAGA
  GTGTTAGCCTGTGGTGGAGATGGAACCGTGGGCTGGGTTTTGGATTGCATAGAAAAGGCCAATGTAGGCA
  AGCATCCTCCAGTTGCGATTCTGCCTCTTGGGACTGGCAATGATCTAGCAAGATGCCTGCGATGGGGAGG
  AGGTTACGAAGGTGAGAATCTGATGAAAATTCTAAAAGACATTGAAAACAGCACAGAAATCATGTTGGAC
  AGGTGGAAGTTTGAAGTCATACCTAATGACAAAGATGAGAAAGGAGACCCAGTGCCTTACAGTATCATCA
  ATAATTACTTTTCCATTGGCGTGGATGCCTCCATTGCACACAGATTCCACATCATGAGAGAAAAACACCC
  AGAGAAATTCAACAGTAGAATGAAGAACAAATTTTGGTATTTTGAGTTTGGCACATCTGAAACTTTCTCA
  GCCACCTGCAAGAAGCTACATGAATCTGTAGAAATAGAATGTGATGGAGTACAGATAGATTTAATAAACA
  TCTCTCTGGAAGGAATTGCTATTTTGAATATACCAAGCATGCATGGAGGATCCAATCTTTGGGGAGAGTC
  TAAGAAAAGACGAAGCCATCGACGAATAGAGAAAAAAGGGTCTGACAAAAGGACCACCGTCACAGATGCC
  AAAGAGTTGAAGTTTGCAAGTCAAGATCTCAGTGACCAGCTGCTGGAGGTGGTCGGCTTGGAAGGAGCCA
  TGGAGATGGGGCAAATATACACAGGCCTGAAAAGTGCTGGCCGGCGGCTGGCTCAGTGCTCCTGCGTGGT
  CATCAGGACGAGCAAGTCTCTGCCAATGCAAATTGATGGGGAGCCATGGATGCAGACCCCATGCACAGTG
  AGTACAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211233 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGKQDILNQTIDFEGFKLFMKTF
  LEAELPDDFTAHLFMSFSNKFPHSSPMVKSKPALLSGGLRMNKGAITPPRTTSPANTCSPEVIHLKDIVC
  YLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIISQMMHVAEYLEWDVTELNPILHEMMEEIDYDH
  DGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSFCKY
  TVHERCVARAPPSCIKTYVKSKRNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQITLHNKC
  ASHLKPECDCGPLKDHILPPTTICPVVLQTLPTSGVSVPEERQSTVKKEKSGSQQPNKVIDKNKMQRANS
  VTVDGQGLQVTPVPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLNFFRDVPDFR
  VLACGGDGTVGWVLDCIEKANVGKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIENSTEIMLD
  RWKFEVIPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFS
  ATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKRRSHRRIEKKGSDKRTTVTDA
  KELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSCVVIRTSKSLPMQIDGEPWMQTPCTV
  STE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145695
• ORF Size: 2319 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145695.1, NM_145695.2, NP_663733.1
• RefSeq Size: 3360 bp
• RefSeq ORF: 2322 bp
• Locus ID: 1607
• Cytogenetics: 7p21.2
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 87.1 kDa
• Gene Summary: 'Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017]'