DGKB (NM_145695) Human Untagged Clone

CAT#: SC306260

DGKB (untagged)-Human diacylglycerol kinase, beta 90kDa (DGKB), transcript variant 2


  "NM_145695" in other vectors (4)

Reconstitution Protocol

USD 760.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol DGKB
Synonyms DAGK2; DGK; DGK-BETA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_145695, the custom clone sequence may differ by one or more nucleotides


ATGACAAACCAGGAAAAATGGGCCCACCTCAGCCCTTCGGAATTTTCCCAACTTCAGAAATATGCTGAGT
ATTCTACAAAGAAATTAAAGGATGTTCTTGAAGAATTCCATGGTAATGGTGTGCTTGCAAAGTATAATCC
TGAAGGGAAACAAGACATTCTTAACCAAACAATAGATTTTGAAGGTTTCAAACTATTCATGAAGACATTC
CTGGAAGCCGAGCTTCCTGATGATTTCACTGCACACCTTTTCATGTCATTTAGCAACAAGTTTCCTCATT
CTAGTCCAATGGTAAAAAGTAAGCCTGCTCTCCTATCAGGCGGTCTGAGAATGAATAAAGGTGCCATCAC
CCCTCCCCGGACTACTTCTCCTGCAAATACGTGTTCCCCAGAAGTAATCCATCTGAAGGACATTGTCTGT
TACCTGTCTCTGCTTGAAAGAGGAAGACCTGAGGATAAGCTTGAGTTTATGTTTCGCCTTTATGACACGG
ATGGGAATGGCTTCCTGGACAGCTCGGAGCTAGAAAATATCATCAGTCAGATGATGCATGTTGCAGAATA
CCTTGAGTGGGATGTCACTGAACTTAATCCAATCCTCCATGAAATGATGGAAGAAATTGACTATGATCAT
GATGGAACCGTGTCTCTGGAGGAATGGATTCAAGGAGGAATGACAACGATTCCACTTCTTGTGCTCCTGG
GCTTAGAAAATAACGTGAAGGATGATGGACAGCACGTGTGGCGACTGAAGCACTTTAACAAACCTGCCTA
TTGCAACCTTTGCCTGAACATGCTGATTGGCGTGGGGAAGCAGGGCCTCTGCTGTTCCTTCTGCAAGTAC
ACAGTCCATGAGCGCTGTGTGGCTCGAGCACCTCCCTCTTGCATCAAGACCTATGTGAAGTCCAAAAGGA
ACACTGATGTCATGCACCATTACTGGGTTGAAGGTAACTGCCCAACCAAGTGTGATAAGTGCCACAAAAC
TGTTAAATGTTACCAGGGCCTGACAGGACTGCATTGTGTTTGGTGTCAGATCACACTGCATAATAAATGT
GCTTCTCATCTAAAACCTGAATGTGACTGTGGACCTTTGAAGGACCATATTTTACCACCCACAACAATCT
GTCCAGTGGTACTGCAGACTCTGCCCACTTCAGGAGTTTCAGTTCCTGAGGAAAGACAATCAACAGTGAA
AAAGGAAAAGAGTGGTTCCCAGCAGCCAAACAAAGTGATTGACAAGAATAAAATGCAAAGAGCCAACTCT
GTTACTGTAGATGGACAAGGCCTGCAGGTCACTCCTGTGCCTGGTACTCACCCACTTTTAGTTTTTGTGA
ACCCCAAAAGTGGTGGAAAACAAGGAGAACGAATTTACAGAAAATTCCAGTATCTATTAAATCCTCGTCA
GGTTTACAGTCTTTCTGGAAATGGACCAATGCCAGGGTTAAACTTTTTCCGTGATGTTCCTGACTTCAGA
GTGTTAGCCTGTGGTGGAGATGGAACCGTGGGCTGGGTTTTGGATTGCATAGAAAAGGCCAATGTAGGCA
AGCATCCTCCAGTTGCGATTCTGCCTCTTGGGACTGGCAATGATCTAGCAAGATGCCTGCGATGGGGAGG
AGGTTACGAAGGTGAGAATCTGATGAAAATTCTAAAAGACATTGAAAACAGCACAGAAATCATGTTGGAC
AGGTGGAAGTTTGAAGTCATACCTAATGACAAAGATGAGAAAGGAGACCCAGTGCCTTACAGTATCATCA
ATAATTACTTTTCCATTGGCGTGGATGCCTCCATTGCACACAGATTCCACATCATGAGAGAAAAACACCC
AGAGAAATTCAACAGTAGAATGAAGAACAAATTTTGGTATTTTGAGTTTGGCACATCTGAAACTTTCTCA
GCCACCTGCAAGAAGCTACATGAATCTGTAGAAATAGAATGTGATGGAGTACAGATAGATTTAATAAACA
TCTCTCTGGAAGGAATTGCTATTTTGAATATACCAAGCATGCATGGAGGATCCAATCTTTGGGGAGAGTC
TAAGAAAAGACGAAGCCATCGACGAATAGAGAAAAAAGGGTCTGACAAAAGGACCACCGTCACAGATGCC
AAAGAGTTGAAGTTTGCAAGTCAAGATCTCAGTGACCAGCTGCTGGAGGTGGTCGGCTTGGAAGGAGCCA
TGGAGATGGGGCAAATATACACAGGCCTGAAAAGTGCTGGCCGGCGGCTGGCTCAGTGCTCCTGCGTGGT
CATCAGGACGAGCAAGTCTCTGCCAATGCAAATTGATGGGGAGCCATGGATGCAGACCCCATGCACAGTG
AGTACAGAGTAG


Restriction Sites SgfI-MluI     
ACCN NM_145695
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_145695.2, NP_663733.1
RefSeq Size 3360 bp
RefSeq ORF 2322 bp
Locus ID 1607
Cytogenetics 7p21.2
Protein Families Druggable Genome
Protein Pathways Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
Gene Summary 'Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017]'
Transcript Variant: This variant (2) has a distinct 3' UTR and 3' coding region compared to variant (1). The encoded protein (2) has a shorter, alternate C-terminus compared to isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.