KCTD16 (NM_020768) Human Tagged ORF Clone

CAT#: RC211283

KCTD16 (Myc-DDK-tagged)-Human potassium channel tetramerisation domain containing 16 (KCTD16)

Product Images

Western blot validation of overexpression lysate (Cat# LY412323) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211283 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KCTD16 protein (Cat# TP311283). The protein was produced from HEK293T cells transfected with KCTD16 cDNA clone (Cat# RC211283) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCTD16
• Synonyms: DKFZp781A1155; KIAA1317; MGC138167
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211283 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGAGTGGAAACTGTAGTCGTTATTATCCTCGAGAACAAGGGTCCGCAGTTCCCAACTCCTTCC
  CTGAGGTGGTAGAGCTGAATGTCGGGGGTCAAGTTTATTTTACTCGCCATTCCACATTGATAAGCATCCC
  TCATTCCCTCCTGTGGAAAATGTTTTCCCCAAAGAGAGACACGGCTAATGATCTAGCCAAGGACTCCAAG
  GGAAGGTTTTTCATTGACAGAGATGGATTCTTGTTCCGTTATATTCTGGACTATCTCAGGGACAGGCAGG
  TGGTCCTGCCTGATCACTTTCCAGAAAAAGGAAGACTGAAAAGGGAAGCTGAATACTTCCAGCTCCCAGA
  CTTGGTCAAACTCCTGACCCCCGATGAAATCAAGCAAAGCCCAGATGAATTCTGCCACAGTGACTTTGAA
  GATGCCTCCCAAGGAAGCGACACAAGAATCTGCCCCCCTTCCTCCCTGCTCCCTGCCGACCGCAAGTGGG
  GTTTCATTACTGTGGGTTACAGAGGATCCTGCACCTTGGGCAGAGAGGGACAGGCAGATGCCAAGTTTCG
  GAGAGTTCCCCGGATTTTGGTTTGTGGAAGGATTTCCTTGGCAAAAGAAGTCTTTGGAGAAACTTTGAAT
  GAAAGCAGAGACCCTGATCGAGCCCCAGAAAGATACACCTCCAGATTTTATCTCAAATTCAAGCACCTGG
  AAAGGGCTTTTGATATGTTGTCAGAGTGTGGATTCCACATGGTGGCCTGTAACTCATCGGTGACAGCATC
  TTTCATCAACCAATATACAGATGACAAGATCTGGTCAAGCTACACTGAATATGTCTTCTACCGTGAGCCT
  TCCAGATGGTCACCCTCACACTGCGATTGCTGCTGCAAGAATGGCAAAGGTGACAAAGAAGGGGAGAGCG
  GCACGTCTTGCAATGACCTCTCCACATCTAGCTGCGACAGCCAGTCTGAGGCCAGCTCTCCCCAGGAGAC
  GGTCATCTGTGGTCCCGTGACACGCCAGACCAACATCCAGACTCTGGACCGTCCCATCAAGAAGGGCCCT
  GTCCAGCTGATCCAACAGTCAGAGATGCGGCGGAAAAGCGACTTACTCCGGACTCTGACTTCAGGCTCCA
  GGGAATCGAACATGAGCAGCAAAAAAAAAGCTGTTAAAGAAAAGCTCTCAATTGAGGAGGAGCTGGAGAA
  ATGTATCCAGGATTTCCTAAAAATCAAAATTCCAGATCGGTTTCCTGAGAGAAAACATCCTTGGCAATCT
  GAACTTTTAAGGAAGTATCATCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211283 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALSGNCSRYYPREQGSAVPNSFPEVVELNVGGQVYFTRHSTLISIPHSLLWKMFSPKRDTANDLAKDSK
  GRFFIDRDGFLFRYILDYLRDRQVVLPDHFPEKGRLKREAEYFQLPDLVKLLTPDEIKQSPDEFCHSDFE
  DASQGSDTRICPPSSLLPADRKWGFITVGYRGSCTLGREGQADAKFRRVPRILVCGRISLAKEVFGETLN
  ESRDPDRAPERYTSRFYLKFKHLERAFDMLSECGFHMVACNSSVTASFINQYTDDKIWSSYTEYVFYREP
  SRWSPSHCDCCCKNGKGDKEGESGTSCNDLSTSSCDSQSEASSPQETVICGPVTRQTNIQTLDRPIKKGP
  VQLIQQSEMRRKSDLLRTLTSGSRESNMSSKKKAVKEKLSIEEELEKCIQDFLKIKIPDRFPERKHPWQS
  ELLRKYHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020768
• ORF Size: 1284 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020768.1, NM_020768.2, NM_020768.3, NP_065819.1
• RefSeq Size: 5183 bp
• RefSeq ORF: 1287 bp
• Locus ID: 57528
• MW: 49.1 kDa