PCDHA4 (NM_031500) Human Tagged ORF Clone

CAT#: RC211327

PCDHA4 (Myc-DDK-tagged)-Human protocadherin alpha 4 (PCDHA4), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY410492) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211327 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PCDHA4 protein (Cat# TP311327). The protein was produced from HEK293T cells transfected with PCDHA4 cDNA clone (Cat# RC211327) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDHA4
• Synonyms: CNR1; CNRN1; CRNR1; PCDH-ALPHA4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211327 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTTTCCTGGGGAAGCGGCCAGGAATCCCGGCGTCTGCTGCTCTTACTTCTTCTCCTCGCAGCCT
  GGGAGGCAGGGAACGGTCAGCTCCACTACTCGGTCTCCGAGGAGGCCAAACACGGCACCTTCGTGGGCCG
  CATCGCGCAGGACCTGGGACTGGAGCTGGCGGAGCTGGTGCCGCGCCTGTTCCGGGTGGCGTCCAAGGGC
  CGCGGAGGCCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTCTCGGATCGACCGGGAGG
  AGCTGTGCCGGCGGAGCGCGGAGTGCAGCATCCACCTGGAGGTGATCGTAGACAGGCCGCTGCAGGTTTT
  CCATGTGGACGTGGAGGTGAGGGACATTAACGATAACCCGCCGGTGTTCCCAGCAACACAAAAGAACCTG
  TCCATCGCGGAATCCAGGCCGCTTGACTCTCGGTTTCCACTAGAGGGCGCCTCGGATGCAGATATCGGGG
  AGAACGCCCTGCTCACTTACAGACTGAGCCCAAATGAATACTTTTCTCTGGAAAAACCACCTGATGACGA
  GCTGGTAAAAGGTCTTGGGCTTATATTACGGAAATCTTTAGACAGAGAAGAAGCTCCGGAGATTTTTTTA
  GTGCTCACAGCCACTGATGGAGGCAAACCCGAGTTGACTGGCACCGTTCAGTTACTCATCACAGTACTGG
  ATGCCAATGACAATGCCCCAGCTTTTGACAGAACCATTTATAAGGTGAGATTACTAGAAAATGTTCCTAA
  TGGAACATTGGTAATTAAACTTAACGCCTCAGATTTAGACGAAGGATTGAATGGGGACATTGTTTATTCA
  TTCTCAAATGATATTTCGCCAAATGTGAAATCCAAGTTTCACATAGATCCAATTACTGGACAAATTATTG
  TAAAGGGATATATTGACTTTGAAGAAAGCAAATCCTATGAAATTATTGTAGAGGGCATTGATAAGGGACA
  GCTCCCACTTTCTGGCCATTGTAGAGTTATTGTGGAAGTAGAAGACAACAACGATAATGTCCCAGATTTG
  GAATTCAAGTCTTTATCACTTCCAATTAGAGAGGACGCTCCACTGGGTACAGTCATCGCCCTGATCAGCG
  TGTCCGACAAAGACATGGGTGTCAATGGGCTGGTCACCTGCTCCTTGACGTCCCACGTCCCCTTCAAGCT
  GGTGTCCACCTTCAAGAATTACTACTCGTTGGTGCTGGACAGTGCCCTGGACCGCGAGAGCGTGTCAGCC
  TATGAGCTGGTGGTGACCGCGCGAGACGGGGGCTCGCCTTCGCTGTGGGCCACGGCCAGTGTTTCTGTGG
  AGGTGGCTGATGTGAACGACAACGCTCCGGCGTTCGCGCAGCCCGAGTACACAGTGTTCGTGAAGGAGAA
  CAACCCGCCGGGCTGCCACATCTTCACTGTGTCTGCGTGGGACGCGGACGCGCAGGAGAACGCGCTGGTG
  TCCTACTCGCTGGTAGAGCGGCGGGTAGGGGAGCGCGCGCTGTCGAGCTACGTTTCGGTGCATGCGGAGA
  GCGGCAAGGTGTACGCGCTGCAGCCGCTGGACCACGAGGAGCTAGAGCTGCTGCAGTTTCAGGTGACCGC
  TCGCGATGCCGGCGTGCCACCTCTGGGCAGCAACGTGACGCTGCAGGTGTTCGTGCTGGACGAAAACGAC
  AACGCGCCAGCACTGCTAGCGCCTCGGGCGGGTGGCACTGGTGGCGCAGTGAGCGAGCTGGTGCCATGGT
  CGGTGGGTGTGGGCCACGTGGTGGCAAAGGTGCGCGCGGTGGATGCTGACTCGGGCTACAACGCGTGGCT
  TTCGTACGAGCTGCAGCCGGGGACTGGTGGCGCGCGCATCCCGTTCCGCGTGGGGCTGTACACTGGCGAG
  ATCAGCACAACGCGTGCCCTGGACGAAACGGACGCTCCGCGCCACCGCCTACTGGTACTGGTGAAGGACC
  ACGGCGAGCCCGCGCTGACGGCCACGGCCACTGTGCTGGTGTCACTTGTGGAGAGTGGACAGGCGCCAAA
  GGCCTCCTCACGGGCGTTGGTGGGCGCTGTGGGTCCCGATGCTGCGCTGGTGGATGTCAACGTATACCTG
  ATCATTGCCATCTGCGCGGTGTCCAGCCTTTTGGTGCTCACGCTGCTGCTGTACACCGCGCTGCGGTGCT
  CTGCGCTGCCCACCGAGGGCGCGTGCGCTCCGGGCAAGCCCACGCTGGTGTGCTCCAGTGCGGTGGGGAG
  CTGGTCATACTCGCAGCAGAGGAGGCCGAGGGTGTGCTCTGGTGAGGGCCCACCCAAGACCGACCTCATG
  GCCTTCAGCCCCAGTTTACCTGACTCTAGGGACAGAGAAGATCAGCTGCAGACAACTGAGGAATCCTTTG
  CAAAGGTTAGTGTA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211327 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEFSWGSGQESRRLLLLLLLLAAWEAGNGQLHYSVSEEAKHGTFVGRIAQDLGLELAELVPRLFRVASKG
  RGGLLEVNLQNGILFVNSRIDREELCRRSAECSIHLEVIVDRPLQVFHVDVEVRDINDNPPVFPATQKNL
  SIAESRPLDSRFPLEGASDADIGENALLTYRLSPNEYFSLEKPPDDELVKGLGLILRKSLDREEAPEIFL
  VLTATDGGKPELTGTVQLLITVLDANDNAPAFDRTIYKVRLLENVPNGTLVIKLNASDLDEGLNGDIVYS
  FSNDISPNVKSKFHIDPITGQIIVKGYIDFEESKSYEIIVEGIDKGQLPLSGHCRVIVEVEDNNDNVPDL
  EFKSLSLPIREDAPLGTVIALISVSDKDMGVNGLVTCSLTSHVPFKLVSTFKNYYSLVLDSALDRESVSA
  YELVVTARDGGSPSLWATASVSVEVADVNDNAPAFAQPEYTVFVKENNPPGCHIFTVSAWDADAQENALV
  SYSLVERRVGERALSSYVSVHAESGKVYALQPLDHEELELLQFQVTARDAGVPPLGSNVTLQVFVLDEND
  NAPALLAPRAGGTGGAVSELVPWSVGVGHVVAKVRAVDADSGYNAWLSYELQPGTGGARIPFRVGLYTGE
  ISTTRALDETDAPRHRLLVLVKDHGEPALTATATVLVSLVESGQAPKASSRALVGAVGPDAALVDVNVYL
  IIAICAVSSLLVLTLLLYTALRCSALPTEGACAPGKPTLVCSSAVGSWSYSQQRRPRVCSGEGPPKTDLM
  AFSPSLPDSRDREDQLQTTEESFAKVSV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031500
• ORF Size: 2394 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031500.1, NM_031500.2, NP_113688.1
• RefSeq Size: 2594 bp
• RefSeq ORF: 2397 bp
• Locus ID: 56144
• Domains: CA
• Protein Families: Transmembrane
• MW: 86.2 kDa
• Gene Summary: This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]