15 Lipoxygenase 2 (ALOX15B) (NM_001141) Human Tagged ORF Clone

CAT#: RC211380

ALOX15B (Myc-DDK-tagged)-Human arachidonate 15-lipoxygenase, type B (ALOX15B), transcript variant d

Product Images

Western blot validation of overexpression lysate (Cat# LY400457) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211380 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALOX15B
• Synonyms: 15-LOX-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211380 representing NM_001141
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGTTCAGGGTCAGGGTGTCCACCGGAGAAGCCTTCGGGGCTGGCACATGGGACAAAGTGTCTG
  TCAGCATCGTGGGGACCCGGGGAGAGAGCCCCCCACTGCCCCTGGACAATCTCGGCAAGGAGTTCACTGC
  GGGCGCTGAGGAGGACTTCCAGGTGACGCTCCCGGAGGACGTAGGCCGAGTGCTGCTGCTGCGCGTGCAC
  AAGGCGCCCCCAGTGCTGCCCCTGCTGGGGCCCCTGGCCCCGGATGCCTGGTTCTGCCGCTGGTTCCAGC
  TGACACCGCCGCGGGGCGGCCACCTCCTCTTCCCCTGCTACCAGTGGCTGGAGGGGGCGGGGACCCTGGT
  GCTGCAGGAGGGTACAGCCAAGGTGTCCTGGGCAGACCACCACCCTGTGCTCCAGCAACAGCGCCAGGAG
  GAGCTTCAGGCCCGGCAGGAGATGTACCAGTGGAAGGCTTACAACCCAGGTTGGCCTCACTGCCTGGATG
  AAAAGACAGTGGAAGACTTGGAGCTCAATATCAAATACTCCACAGCCAAGAATGCCAACTTTTATCTACA
  GGCTGGCTCTGCTTTTGCAGAGATGAAAATCAAGGGGTTGCTGGACCGCAAGGGGCTCTGGAGGAGTCTG
  AATGAGATGAAAAGGATCTTCAACTTCCGGAGGACCCCAGCAGCTGAGCACGCATTTGAGCACTGGCAGG
  AGGATGCCTTCTTCGCCTCCCAGTTCCTGAATGGTCTCAACCCTGTCCTGATCCGCCGCTGTCACTACCT
  CCCAAAGAACTTCCCCGTCACTGATGCCATGGTGGCCTCAGTGTTGGGTCCTGGGACCAGCTTGCAGGCT
  GAGCTAGAGAAGGGCTCCCTGTTCTTGGTGGATCACGGCATCCTCTCTGGCATCCAGACCAATGTCATTA
  ATGGGAAGCCTCAGTTCTCTGCGGCCCCAATGACCCTGCTATACCAGAGCCCAGGCTGCGGGCCGCTGCT
  GCCTCTCGCCATCCAGCTCAGCCAGACCCCCGGCCCAAACAGCCCCATCTTCCTGCCCACTGATGACAAG
  TGGGACTGGTTGCTGGCCAAGACCTGGGTGCGCAATGCCGAGTTCTCCTTCCATGAGGCCCTCACGCACC
  TGCTGCACTCACATCTGCTGCCTGAGGTCTTCACCCTGGCTACCCTGCGTCAGCTGCCCCACTGCCACCC
  TCTCTTCAAGCTGCTGATCCCGCACACCCGATACACCCTGCACATCAACACACTCGCCCGGGAGCTGCTT
  ATCGTGCCAGGGCAGGTGGTGGACAGGTCCACAGGCATCGGCATTGAAGGCTTCTCTGAGTTGATACAGA
  GGAACATGAAGCAGCTGAACTATTCTCTCCTGTGTCTGCCTGAGGATATCCGGACCCGAGGAGTTGAAGA
  CATCCCAGGCTACTACTACCGTGATGATGGGATGCAGATTTGGGGTGCAGTGGAACGCTTTGTCTCTGAA
  ATCATCGGTATCTACTACCCAAGTGATGAGTCTGTCCAAGATGACAGAGAGCTCCAGGCCTGGGTCAGAG
  AGATCTTCTCCAAGGGCTTCCTAAACCAGGAGAGCTCAGGTATACCCTCCTCACTGGAGACCCGGGAAGC
  CCTGGTGCAGTATGTCACCATGGTGATATTCACCTGCTCAGCCAAGCATGCGGCTGTCAGTGCAGGGCAG
  TTTGACTCCTGTGCTTGGATGCCCAACCTGCCACCCAGCATGCAGCTGCCACCACCCACCTCCAAAGGCC
  TGGCAACATGCGAGGGCTTCATAGCCACCCTCCCACCTGTCAATGCCACATGTGATGTCATCCTTGCTCT
  CTGGTTGCTGAGCAAGGAGCCTGGAGACCAAAGGCCCCTGGGCACCTATCCGGATGAGCACTTCACAGAG
  GAGGCCCCTCGGCGGAGCATCGCCACCTTCCAGAGCCGCCTGGCCCAGATCTCGAGGGGCATCCAGGAGC
  GGAACCGGGGCCTGGTGCTGCCCTACACCTACCTAGACCCTCCCCTCATCGAGAACAGCGTCTCCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211380 representing NM_001141
  Red=Cloning site Green=Tags(s)
  
  MAEFRVRVSTGEAFGAGTWDKVSVSIVGTRGESPPLPLDNLGKEFTAGAEEDFQVTLPEDVGRVLLLRVH
  KAPPVLPLLGPLAPDAWFCRWFQLTPPRGGHLLFPCYQWLEGAGTLVLQEGTAKVSWADHHPVLQQQRQE
  ELQARQEMYQWKAYNPGWPHCLDEKTVEDLELNIKYSTAKNANFYLQAGSAFAEMKIKGLLDRKGLWRSL
  NEMKRIFNFRRTPAAEHAFEHWQEDAFFASQFLNGLNPVLIRRCHYLPKNFPVTDAMVASVLGPGTSLQA
  ELEKGSLFLVDHGILSGIQTNVINGKPQFSAAPMTLLYQSPGCGPLLPLAIQLSQTPGPNSPIFLPTDDK
  WDWLLAKTWVRNAEFSFHEALTHLLHSHLLPEVFTLATLRQLPHCHPLFKLLIPHTRYTLHINTLARELL
  IVPGQVVDRSTGIGIEGFSELIQRNMKQLNYSLLCLPEDIRTRGVEDIPGYYYRDDGMQIWGAVERFVSE
  IIGIYYPSDESVQDDRELQAWVREIFSKGFLNQESSGIPSSLETREALVQYVTMVIFTCSAKHAAVSAGQ
  FDSCAWMPNLPPSMQLPPPTSKGLATCEGFIATLPPVNATCDVILALWLLSKEPGDQRPLGTYPDEHFTE
  EAPRRSIATFQSRLAQISRGIQERNRGLVLPYTYLDPPLIENSVSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001141
• ORF Size: 2028 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001141.1, NM_001141.2, NP_001132.1
• RefSeq Size: 2685 bp
• RefSeq ORF: 2031 bp
• Locus ID: 247
• Cytogenetics: 17p13.1
• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• MW: 75.7 kDa
• Gene Summary: 'This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'