PLXNC1 (NM_005761) Human Tagged ORF Clone

CAT#: RC211396

PLXNC1 (Myc-DDK-tagged)-Human plexin C1 (PLXNC1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417085) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211396 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PLXNC1 protein (Cat# TP311396). The protein was produced from HEK293T cells transfected with PLXNC1 cDNA clone (Cat# RC211396) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLXNC1
• Synonyms: CD232; PLXN-C1; VESPR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211396 representing NM_005761
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTCTCCCGGAGGAAGGCGCCGCCGCGCCCCCCGCGCCCCGCAGCGCCACTGCCCCTGCTCGCCT
  ATCTGCTGGCACTGGCGGCTCCCGGCCGGGGCGCGGACGAGCCCGTGTGGCGGTCGGAGCAAGCCATCGG
  AGCCATCGCGGCGAGCCAGGAGGACGGCGTGTTTGTGGCGAGCGGCAGCTGCCTGGACCAGCTGGACTAC
  AGCCTGGAGCACAGCCTCTCGCGCCTGTACCGGGACCAAGCGGGCAACTGCACAGAGCCGGTCTCGCTGG
  CGCCCCCCGCGCGGCCCCGGCCCGGGAGCAGCTTCAGCAAGCTGCTGCTGCCCTACCGCGAGGGGGCGGC
  CGGCCTCGGGGGGCTGCTGCTCACCGGCTGGACCTTCGACCGGGGCGCCTGCGAGGTGCGGCCCCTGGGC
  AACCTGAGCCGCAACTCCCTGCGCAACGGCACCGAGGTGGTGTCGTGCCACCCGCAGGGCTCGACGGCCG
  GCGTGGTGTACCGCGCGGGCCGGAACAACCGCTGGTACCTGGCGGTGGCCGCCACCTACGTGCTGCCTGA
  GCCGGAGACGGCGAGCCGCTGCAACCCCGCGGCATCCGACCACGACACGGCCATCGCGCTCAAGGACACG
  GAGGGGCGCAGCCTGGCCACGCAGGAGCTGGGGCGCCTCAAGCTGTGCGAGGGCGCGGGCAGCCTGCACT
  TCGTGGACGCCTTTCTCTGGAACGGCAGCATCTACTTCCCCTACTACCCCTACAACTATACGAGCGGCGC
  TGCCACCGGCTGGCCCAGCATGGCGCGCATCGCGCAGAGCACCGAGGTGCTGTTCCAGGGCCAGGCATCC
  CTCGACTGCGGCCACGGCCACCCCGACGGCCGCCGCCTGCTCCTCTCCTCCAGCCTAGTGGAGGCCCTGG
  ACGTCTGGGCGGGAGTGTTCAGCGCGGCCGCTGGAGAGGGCCAGGAGCGGCGCTCCCCCACCACCACGGC
  GCTCTGCCTCTTCAGAATGAGTGAGATCCAGGCGCGCGCCAAGAGGGTCAGCTGGGACTTCAAGACGGCC
  GAGAGCCACTGCAAAGAAGGGGATCAACCTGAAAGAGTCCAACCAATCGCATCATCTACCTTGATCCATT
  CCGACCTGACATCCGTTTATGGCACCGTGGTAATGAACAGGACTGTTTTATTCTTGGGGACTGGAGATGG
  CCAGTTACTTAAGGTTATTCTTGGTGAGAATTTGACTTCAAATTGTCCAGAGGTTATCTATGAAATTAAA
  GAAGAGACACCTGTTTTCTACAAACTCGTTCCTGATCCTGTGAAGAATATCTACATTTATCTAACAGCTG
  GGAAAGAGGTGAGGAGAATTCGTGTTGCAAACTGCAATAAACATAAATCCTGTTCGGAGTGTTTAACAGC
  CACAGACCCTCACTGCGGTTGGTGCCATTCGCTACAAAGGTGCACTTTTCAAGGAGATTGTGTACATTCA
  GAGAACTTAGAAAACTGGCTGGATATTTCGTCTGGAGCAAAAAAGTGCCCTAAAATTCAGATAATTCGAA
  GCAGTAAAGAAAAGACTACAGTGACTATGGTGGGAAGCTTCTCTCCAAGACACTCAAAGTGCATGGTGAA
  GAATGTGGACTCTAGCAGGGAGCTCTGCCAGAATAAAAGTCAGCCCAACCGGACCTGCACCTGTAGCATC
  CCAACCAGAGCAACCTACAAAGATGTTTCAGTTGTCAACGTGATGTTCTCCTTCGGTTCTTGGAATTTAT
  CAGACAGATTCAACTTTACCAACTGCTCATCATTAAAAGAATGCCCAGCATGCGTAGAAACTGGCTGCGC
  GTGGTGTAAAAGTGCAAGAAGGTGTATCCACCCCTTCACAGCTTGCGACCCTTCTGATTATGAGAGAAAC
  CAGGAACAGTGTCCAGTGGCTGTCGAGAAGACATCAGGAGGAGGAAGACCCAAGGAGAACAAGGGGAACA
  GAACCAACCAGGCTTTACAGGTCTTCTACATTAAGTCCATTGAGCCACAGAAAGTATCGACATTAGGGAA
  AAGCAACGTGATAGTAACGGGAGCAAACTTTACCCGGGCATCGAACATCACAATGATCCTGAAAGGAACC
  AGTACCTGTGATAAGGATGTGATACAGGTTAGCCATGTGCTAAATGACACCCACATGAAATTCTCTCTTC
  CATCAAGCCGGAAAGAAATGAAGGATGTGTGTATCCAGTTTGATGGTGGGAACTGCTCTTCTGTGGGATC
  CTTATCCTACATTGCTCTGCCACATTGTTCCCTTATATTTCCTGCTACCACCTGGATCAGTGGTGGTCAA
  AATATAACCATGATGGGCAGAAATTTTGATGTAATTGACAACTTAATCATTTCACATGAATTAAAAGGAA
  ACATAAATGTCTCTGAATATTGTGTGGCGACTTACTGCGGGTTTTTAGCCCCCAGTTTAAAGAGTTCAAA
  AGTGCGCACGAATGTCACTGTGAAGCTGAGAGTACAAGACACCTACTTGGATTGTGGAACCCTGCAGTAT
  CGGGAGGACCCCAGATTCACGGGGTATCGGGTGGAATCCGAGGTGGACACAGAACTGGAAGTGAAAATTC
  AAAAAGAAAATGACAACTTCAATATTTCCAAAAAAGACATTGAAATTACTCTCTTCCATGGGGAAAATGG
  GCAATTAAATTGCAGTTTTGAAAATATTACTAGAAATCAAGATCTTACCACCATCCTTTGCAAAATTAAA
  GGCATCAAGACTGCAAGCACCATTGCCAACTCTTCTAAGAAAGTTCGGGTCAAGCTGGGAAACCTGGAGC
  TCTACGTCGAGCAGGAGTCAGTTCCTTCCACATGGTATTTTCTGATTGTGCTCCCTGTCTTGCTAGTGAT
  TGTCATTTTTGCGGCCGTGGGGGTGACCAGGCACAAATCGAAGGAGCTGAGTCGCAAACAGAGTCAACAA
  CTAGAATTGCTGGAAAGCGAGCTCCGGAAAGAGATACGTGACGGCTTTGCTGAGCTGCAGATGGATAAAT
  TGGATGTGGTTGATAGTTTTGGAACTGTTCCCTTCCTTGACTACAAACATTTTGCTCTGAGAACTTTCTT
  CCCTGAGTCAGGTGGCTTCACCCACATCTTCACTGAAGATATGCATAACAGAGACGCCAACGACAAGAAT
  GAAAGTCTCACAGCTTTGGATGCCCTAATCTGTAATAAAAGCTTTCTTGTTACTGTCATCCACACCCTTG
  AAAAGCAGAAGAACTTTTCTGTGAAGGACAGGTGTCTGTTTGCCTCCTTCCTAACCATTGCACTGCAAAC
  CAAGCTGGTCTACCTGACCAGCATCCTAGAGGTGCTGACCAGGGACTTGATGGAACAGTGTAGTAACATG
  CAGCCGAAACTCATGCTGAGACGCACGGAGTCCGTCGTCGAAAAACTCCTCACAAACTGGATGTCCGTCT
  GCCTTTCTGGATTTCTCCGGGAGACTGTCGGAGAGCCCTTCTATTTGCTGGTGACGACTCTGAACCAGAA
  AATTAACAAGGGTCCCGTGGATGTAATCACTTGCAAAGCCCTGTACACACTTAATGAAGACTGGCTGTTG
  TGGCAGGTTCCGGAATTCAGTACTGTGGCATTAAACGTCGTCTTTGAAAAAATCCCGGAAAACGAGAGTG
  CAGATGTCTGTCGGAATATTTCAGTCAATGTTCTCGACTGTGACACCATTGGCCAAGCCAAAGAAAAGAT
  TTTCCAAGCATTCTTAAGCAAAAATGGCTCTCCTTATGGACTTCAGCTTAATGAAATTGGTCTTGAGCTT
  CAAATGGGCACACGACAGAAAGAACTTCTGGACATCGACAGTTCCTCCGTGATTCTTGAAGATGGAATCA
  CCAAGCTAAACACCATTGGCCACTATGAGATATCAAATGGATCCACTATAAAAGTCTTTAAGAAGATAGC
  AAATTTTACTTCAGATGTGGAGTACTCGGATGACCACTGCCATTTGATTTTACCAGATTCGGAAGCATTC
  CAAGATGTGCAAGGAAAGAGACATCGAGGGAAGCACAAGTTCAAAGTAAAAGAAATGTATCTGACAAAGC
  TGCTGTCGACCAAGGTGGCAATTCATTCTGTGCTTGAAAAACTTTTTAGAAGCATTTGGAGTTTACCCAA
  CAGCAGAGCTCCATTTGCTATAAAATACTTTTTTGACTTTTTGGACGCCCAGGCTGAAAACAAAAAAATC
  ACAGATCCTGACGTCGTACATATTTGGAAAACAAACAGCCTTCCTCTTCGCTTCTGGGTAAACATCCTGA
  AGAACCCTCAGTTTGTCTTTGACATTAAGAAGACACCACATATAGACGGCTGTTTGTCAGTGATTGCCCA
  GGCATTCATGGATGCATTTTCTCTCACAGAGCAGCAACTAGGGAAGGAAGCACCAACTAATAAGCTTCTC
  TATGCCAAGGATATCCCAACCTACAAAGAAGAAGTAAAATCTTATTACAAAGCAATCAGGGATTTGCCTC
  CATTGTCATCCTCAGAAATGGAAGAATTTTTAACTCAGGAATCTAAGAAACATGAAAATGAATTTAATGA
  AGAAGTGGCCTTGACAGAAATTTACAAATACATCGTAAAATATTTTGATGAGATTCTAAATAAACTAGAA
  AGAGAACGAGGGCTGGAAGAAGCTCAGAAACAACTCTTGCATGTAAAAGTCTTATTTGATGAAAAGAAGA
  AATGCAAGTGGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211396 representing NM_005761
  Red=Cloning site Green=Tags(s)
  
  MEVSRRKAPPRPPRPAAPLPLLAYLLALAAPGRGADEPVWRSEQAIGAIAASQEDGVFVASGSCLDQLDY
  SLEHSLSRLYRDQAGNCTEPVSLAPPARPRPGSSFSKLLLPYREGAAGLGGLLLTGWTFDRGACEVRPLG
  NLSRNSLRNGTEVVSCHPQGSTAGVVYRAGRNNRWYLAVAATYVLPEPETASRCNPAASDHDTAIALKDT
  EGRSLATQELGRLKLCEGAGSLHFVDAFLWNGSIYFPYYPYNYTSGAATGWPSMARIAQSTEVLFQGQAS
  LDCGHGHPDGRRLLLSSSLVEALDVWAGVFSAAAGEGQERRSPTTTALCLFRMSEIQARAKRVSWDFKTA
  ESHCKEGDQPERVQPIASSTLIHSDLTSVYGTVVMNRTVLFLGTGDGQLLKVILGENLTSNCPEVIYEIK
  EETPVFYKLVPDPVKNIYIYLTAGKEVRRIRVANCNKHKSCSECLTATDPHCGWCHSLQRCTFQGDCVHS
  ENLENWLDISSGAKKCPKIQIIRSSKEKTTVTMVGSFSPRHSKCMVKNVDSSRELCQNKSQPNRTCTCSI
  PTRATYKDVSVVNVMFSFGSWNLSDRFNFTNCSSLKECPACVETGCAWCKSARRCIHPFTACDPSDYERN
  QEQCPVAVEKTSGGGRPKENKGNRTNQALQVFYIKSIEPQKVSTLGKSNVIVTGANFTRASNITMILKGT
  STCDKDVIQVSHVLNDTHMKFSLPSSRKEMKDVCIQFDGGNCSSVGSLSYIALPHCSLIFPATTWISGGQ
  NITMMGRNFDVIDNLIISHELKGNINVSEYCVATYCGFLAPSLKSSKVRTNVTVKLRVQDTYLDCGTLQY
  REDPRFTGYRVESEVDTELEVKIQKENDNFNISKKDIEITLFHGENGQLNCSFENITRNQDLTTILCKIK
  GIKTASTIANSSKKVRVKLGNLELYVEQESVPSTWYFLIVLPVLLVIVIFAAVGVTRHKSKELSRKQSQQ
  LELLESELRKEIRDGFAELQMDKLDVVDSFGTVPFLDYKHFALRTFFPESGGFTHIFTEDMHNRDANDKN
  ESLTALDALICNKSFLVTVIHTLEKQKNFSVKDRCLFASFLTIALQTKLVYLTSILEVLTRDLMEQCSNM
  QPKLMLRRTESVVEKLLTNWMSVCLSGFLRETVGEPFYLLVTTLNQKINKGPVDVITCKALYTLNEDWLL
  WQVPEFSTVALNVVFEKIPENESADVCRNISVNVLDCDTIGQAKEKIFQAFLSKNGSPYGLQLNEIGLEL
  QMGTRQKELLDIDSSSVILEDGITKLNTIGHYEISNGSTIKVFKKIANFTSDVEYSDDHCHLILPDSEAF
  QDVQGKRHRGKHKFKVKEMYLTKLLSTKVAIHSVLEKLFRSIWSLPNSRAPFAIKYFFDFLDAQAENKKI
  TDPDVVHIWKTNSLPLRFWVNILKNPQFVFDIKKTPHIDGCLSVIAQAFMDAFSLTEQQLGKEAPTNKLL
  YAKDIPTYKEEVKSYYKAIRDLPPLSSSEMEEFLTQESKKHENEFNEEVALTEIYKYIVKYFDEILNKLE
  RERGLEEAQKQLLHVKVLFDEKKKCKWM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005761
• ORF Size: 4704 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005761.1, NM_005761.2, NP_005752.1
• RefSeq Size: 5121 bp
• RefSeq ORF: 4707 bp
• Locus ID: 10154
• Domains: Sema, PSI, IPT, PSI
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance
• MW: 175.6 kDa
• Gene Summary: This gene encodes a member of the plexin family. Plexins are transmembrane receptors for semaphorins, a large family of proteins that regulate axon guidance, cell motility and migration, and the immune response. The encoded protein and its ligand regulate melanocyte adhesion, and viral semaphorins may modulate the immune response by binding to this receptor. The encoded protein may be a tumor suppressor protein for melanoma. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2011]