HIP1 (NM_005338) Human Tagged ORF Clone
CAT#: RC211418
HIP1 (Myc-DDK-tagged)-Human huntingtin interacting protein 1 (HIP1)
"NM_005338" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | HIP1 |
Synonyms | HIP-I; ILWEQ; SHON; SHONbeta; SHONgamma |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC211418 representing NM_005338
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCGGATGGCCAGCTCCATGAAGCAGGTGCCCAACCCACTGCCCAAGGTGCTGAGCCGGCGCGGGG TCGGCGCTGGGCTGGAGGCGGCGGAGCGCGAGAGCTTCGAGCGGACTCAGACTGTCAGCATCAATAAGGC CATTAATACGCAGGAAGTGGCTGTAAAGGAAAAACACGCCAGAACGTGCATACTGGGCACCCACCATGAG AAAGGGGCACAGACCTTCTGGTCTGTTGTCAACCGCCTGCCTCTGTCTAGCAACGCAGTGCTCTGCTGGA AGTTCTGCCATGTGTTCCACAAACTCCTCCGAGATGGACACCCGAACGTCCTGAAGGACTCTCTGAGATA CAGAAATGAATTGAGTGACATGAGCAGGATGTGGGGCCACCTGAGCGAGGGGTATGGCCAGCTGTGCAGC ATCTACCTGAAACTGCTAAGAACCAAGATGGAGTACCACACCAAAAATCCCAGGTTCCCAGGCAACCTGC AGATGAGTGACCGCCAGCTGGACGAGGCTGGAGAAAGTGACGTGAACAACTTTTTCCAGTTAACAGTGGA GATGTTTGACTACCTGGAGTGTGAACTCAACCTCTTCCAAACAGTATTCAACTCCCTGGACATGTCCCGC TCTGTGTCCGTGACGGCAGCAGGGCAGTGCCGCCTCGCCCCGCTGATCCAGGTCATCTTGGACTGCAGCC ACCTTTATGACTACACTGTCAAGCTTCTCTTCAAACTCCACTCCTGCCTCCCAGCTGACACCCTGCAAGG CCACCGGGACCGCTTCATGGAGCAGTTTACAAAGTTGAAAGATCTGTTCTACCGCTCCAGCAACCTGCAG TACTTCAAGCGGCTCATTCAGATCCCCCAGCTGCCTGAGAACCCACCCAACTTCCTGCGAGCCTCAGCCC TGTCAGAACATATCAGCCCTGTGGTGGTGATCCCTGCAGAGGCCTCATCCCCCGACAGCGAGCCAGTCCT AGAGAAGGATGACCTCATGGACATGGATGCCTCTCAGCAGAATTTATTTGACAACAAGTTTGATGACATC TTTGGCAGTTCATTCAGCAGTGATCCCTTCAATTTCAACAGTCAAAATGGTGTGAACAAGGATGAGAAGG ACCACTTAATTGAGCGACTATACAGAGAGATCAGTGGATTGAAGGCACAGCTAGAAAACATGAAGACTGA GAGCCAGCGGGTTGTGCTGCAGCTGAAGGGCCACGTCAGCGAGCTGGAAGCAGATCTGGCCGAGCAGCAG CACCTGCGGCAGCAGGCGGCCGACGACTGTGAATTCCTGCGGGCAGAACTGGACGAGCTCAGGAGGCAGC GGGAGGACACCGAGAAGGCTCAGCGGAGCCTGTCTGAGATAGAAAGGAAAGCTCAAGCCAATGAACAGCG ATATAGCAAGCTAAAGGAGAAGTACAGCGAGCTGGTTCAGAACCACGCTGACCTGCTGCGGAAGAATGCA GAGGTGACCAAACAGGTGTCCATGGCCAGACAAGCCCAGGTAGATTTGGAACGAGAGAAAAAAGAGCTGG AGGATTCGTTGGAGCGCATCAGTGACCAGGGCCAGCGGAAGACTCAAGAACAGCTGGAAGTTCTAGAGAG CTTGAAGCAGGAACTTGCCACAAGCCAACGGGAGCTTCAGGTTCTGCAAGGCAGCCTGGAAACTTCTGCC CAGTCAGAAGCAAACTGGGCAGCCGAGTTCGCCGAGCTAGAGAAGGAGCGGGACAGCCTGGTGAGTGGCG CAGCTCATAGGGAGGAGGAATTATCTGCTCTTCGGAAAGAACTGCAGGACACTCAGCTCAAACTGGCCAG CACAGAGGAATCTATGTGCCAGCTTGCCAAAGACCAACGAAAAATGCTTCTGGTGGGGTCCAGGAAGGCT GCGGAGCAGGTGATACAAGACGCCCTGAACCAGCTTGAAGAACCTCCTCTCATCAGCTGCGCTGGGTCTG CAGATCACCTCCTCTCCACGGTCACATCCATTTCCAGCTGCATCGAGCAACTGGAGAAAAGCTGGAGCCA GTATCTGGCCTGCCCAGAAGACATCAGTGGACTTCTCCATTCCATAACCCTGCTGGCCCACTTGACCAGC GACGCCATTGCTCATGGTGCCACCACCTGCCTCAGAGCCCCACCTGAGCCTGCCGACTCACTGACCGAGG CCTGTAAGCAGTATGGCAGGGAAACCCTCGCCTACCTGGCCTCCCTGGAGGAAGAGGGAAGCCTTGAGAA TGCCGACAGCACAGCCATGAGGAACTGCCTGAGCAAGATCAAGGCCATCGGCGAGGAGCTCCTGCCCAGG GGACTGGACATCAAGCAGGAGGAGCTGGGGGACCTGGTGGACAAGGAGATGGCGGCCACTTCAGCTGCTA TTGAAACTGCCACGGCCAGAATAGAGGAGATGCTCAGCAAATCCCGAGCAGGAGACACAGGAGTCAAATT GGAGGTGAATGAAAGGATCCTTGGTTGCTGTACCAGCCTCATGCAAGCTATTCAGGTGCTCATCGTGGCC TCTAAGGACCTCCAGAGAGAGATTGTGGAGAGCGGCAGGGGTACAGCATCCCCTAAAGAGTTTTATGCCA AGAACTCTCGATGGACAGAAGGACTTATCTCAGCCTCCAAGGCTGTGGGCTGGGGAGCCACTGTCATGGT GGATGCAGCTGATCTGGTGGTACAAGGCAGAGGGAAATTTGAGGAGCTAATGGTGTGTTCTCATGAAATT GCTGCTAGCACAGCCCAGCTTGTGGCTGCATCCAAGGTGAAAGCTGATAAGGACAGCCCCAACCTAGCCC AGCTGCAGCAGGCCTCTCGGGGAGTGAACCAGGCCACTGCCGGCGTTGTGGCCTCAACCATTTCCGGCAA ATCACAGATCGAAGAGACAGACAACATGGACTTCTCAAGCATGACGCTGACACAGATCAAACGCCAAGAG ATGGATTCTCAGGTTAGGGTGCTAGAGCTAGAAAATGAATTGCAGAAGGAGCGTCAAAAACTGGGAGAGC TTCGGAAAAAGCACTACGAGCTTGCTGGTGTTGCTGAGGGCTGGGAAGAAGGAACAGAGGCATCTCCACC TACACTGCAAGAAGTGGTAACCGAAAAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211418 representing NM_005338
Red=Cloning site Green=Tags(s) MDRMASSMKQVPNPLPKVLSRRGVGAGLEAAERESFERTQTVSINKAINTQEVAVKEKHARTCILGTHHE KGAQTFWSVVNRLPLSSNAVLCWKFCHVFHKLLRDGHPNVLKDSLRYRNELSDMSRMWGHLSEGYGQLCS IYLKLLRTKMEYHTKNPRFPGNLQMSDRQLDEAGESDVNNFFQLTVEMFDYLECELNLFQTVFNSLDMSR SVSVTAAGQCRLAPLIQVILDCSHLYDYTVKLLFKLHSCLPADTLQGHRDRFMEQFTKLKDLFYRSSNLQ YFKRLIQIPQLPENPPNFLRASALSEHISPVVVIPAEASSPDSEPVLEKDDLMDMDASQQNLFDNKFDDI FGSSFSSDPFNFNSQNGVNKDEKDHLIERLYREISGLKAQLENMKTESQRVVLQLKGHVSELEADLAEQQ HLRQQAADDCEFLRAELDELRRQREDTEKAQRSLSEIERKAQANEQRYSKLKEKYSELVQNHADLLRKNA EVTKQVSMARQAQVDLEREKKELEDSLERISDQGQRKTQEQLEVLESLKQELATSQRELQVLQGSLETSA QSEANWAAEFAELEKERDSLVSGAAHREEELSALRKELQDTQLKLASTEESMCQLAKDQRKMLLVGSRKA AEQVIQDALNQLEEPPLISCAGSADHLLSTVTSISSCIEQLEKSWSQYLACPEDISGLLHSITLLAHLTS DAIAHGATTCLRAPPEPADSLTEACKQYGRETLAYLASLEEEGSLENADSTAMRNCLSKIKAIGEELLPR GLDIKQEELGDLVDKEMAATSAAIETATARIEEMLSKSRAGDTGVKLEVNERILGCCTSLMQAIQVLIVA SKDLQREIVESGRGTASPKEFYAKNSRWTEGLISASKAVGWGATVMVDAADLVVQGRGKFEELMVCSHEI AASTAQLVAASKVKADKDSPNLAQLQQASRGVNQATAGVVASTISGKSQIEETDNMDFSSMTLTQIKRQE MDSQVRVLELENELQKERQKLGELRKKHYELAGVAEGWEEGTEASPPTLQEVVTEKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_005338 |
ORF Size | 3111 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005338.4, NM_005338.5, NM_005338.6, NP_005329.3 |
RefSeq Size | 8051 bp |
RefSeq ORF | 3114 bp |
Locus ID | 3092 |
Cytogenetics | 7q11.23 |
Domains | I_LWEQ |
Protein Families | Druggable Genome |
Protein Pathways | Huntington's disease |
MW | 116.2 kDa |
Gene Summary | 'The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC108827 | HIP1 (untagged)-Human huntingtin interacting protein 1 (HIP1) |
USD 1,100.00 |
|
SC318457 | HIP1 (untagged)-Human huntingtin interacting protein 1 (HIP1) |
USD 1,100.00 |
|
RG211418 | HIP1 (GFP-tagged) - Human huntingtin interacting protein 1 (HIP1) |
USD 1,200.00 |
|
RC211418L1 | Lenti-ORF clone of HIP1 (Myc-DDK-tagged)-Human huntingtin interacting protein 1 (HIP1) |
USD 1,290.00 |
|
RC211418L2 | Lenti-ORF clone of HIP1 (mGFP-tagged)-Human huntingtin interacting protein 1 (HIP1) |
USD 1,290.00 |
|
RC211418L3 | Lenti-ORF clone of HIP1 (Myc-DDK-tagged)-Human huntingtin interacting protein 1 (HIP1) |
USD 1,290.00 |
|
RC211418L4 | Lenti-ORF clone of HIP1 (mGFP-tagged)-Human huntingtin interacting protein 1 (HIP1) |
USD 1,290.00 |
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