HIP1 (NM_005338) Human Untagged Clone

CAT#: SC108827

HIP1 (untagged)-Human huntingtin interacting protein 1 (HIP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HIP1
• Synonyms: HIP-I; ILWEQ; SHON; SHONbeta; SHONgamma
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108827 sequence for NM_005338 edited (data generated by NextGen Sequencing)
  ATGGATCGGATGGCCAGCTCCATGAAGCAGGTGCCCAACCCACTGCCCAAGGTGCTGAGC
  CGGCGCGGGGTCGGCGCTGGGCTGGAGGCGGCGGAGCGCGAGAGCTTCGAGCGGACTCAG
  ACTGTCAGCATCAATAAGGCCATTAATACGCAGGAAGTGGCTGTAAAGGAAAAACACGCC
  AGAACGTGCATACTGGGCACCCACCATGAGAAAGGGGCACAGACCTTCTGGTCTGTTGTC
  AACCGCCTGCCTCTGTCTAGCAACGCAGTGCTCTGCTGGAAGTTCTGCCATGTGTTCCAC
  AAACTCCTCCGAGATGGACACCCGAACGTCCTGAAGGACTCTCTGAGATACAGAAATGAA
  TTGAGTGACATGAGCAGGATGTGGGGCCACCTGAGCGAGGGGTATGGCCAGCTGTGCAGC
  ATCTACCTGAAACTGCTAAGAACCAAGATGGAGTACCACACCAAAAATCCCAGGTTCCCA
  GGCAACCTGCAGATGAGTGACCGCCAGCTGGACGAGGCTGGAGAAAGTGACGTGAACAAC
  TTTTTCCAGTTAACAGTGGAGATGTTTGACTACCTGGAGTGTGAACTCAACCTCTTCCAA
  ACAGTATTCAACTCCCTGGACATGTCCCGCTCTGTGTCCGTGACGGCAGCAGGGCAGTGC
  CGCCTCGCCCCGCTGATCCAGGTCATCTTGGACTGCAGCCACCTTTATGACTACACTGTC
  AAGCTTCTCTTCAAACTCCACTCCTGCCTCCCAGCTGACACCCTGCAAGGCCACCGGGAC
  CGCTTCATGGAGCAGTTTACAAAGTTGAAAGATCTGTTCTACCGCTCCAGCAACCTGCAG
  TACTTCAAGCGGCTCATTCAGATCCCCCAGCTGCCTGAGAACCCACCCAACTTCCTGCGA
  GCCTCAGCCCTGTCAGAACATATCAGCCCTGTGGTGGTGATCCCTGCAGAGGCCTCATCC
  CCCGACAGCGAGCCAGTCCTAGAGAAGGATGACCTCATGGACATGGATGCCTCTCAGCAG
  AATTTATTTGACAACAAGTTTGATGACATCTTTGGCAGTTCATTCAGCAGTGATCCCTTC
  AATTTCAACAGTCAAAATGGTGTGAACAAGGATGAGAAGGACCACTTAATTGAGCGACTA
  TACAGAGAGATCAGTGGATTGAAGGCACAGCTAGAAAACATGAAGACTGAGAGCCAGCGG
  GTTGTGCTGCAGCTGAAGGGCCACGTCAGCGAGCTGGAAGCAGATCTGGCCGAGCAGCAG
  CACCTGCGGCAGCAGGCGGCCGACGACTGTGAATTCCTGCGGGCAGAACTGGACGAGCTC
  AGGAGGCAGCGGGAGGACACCGAGAAGGCTCAGCGGAGCCTGTCTGAGATAGAAAGGAAA
  GCTCAAGCCAATGAACAGCGATATAGCAAGCTAAAGGAGAAGTACAGCGAGCTGGTTCAG
  AACCACGCTGACCTGCTGCGGAAGAATGCAGAGGTGACCAAACAGGTGTCCATGGCCAGA
  CAAGCCCAGGTAGATTTGGAACGAGAGAAAAAAGAGCTGGAGGATTCGTTGGAGCGCATC
  AGTGACCAGGGCCAGCGGAAGACTCAAGAACAGCTGGAAGTTCTAGAGAGCTTGAAGCAG
  GAACTTGCCACAAGCCAACGGGAGCTTCAGGTTCTGCAAGGCAGCCTGGAAACTTCTGCC
  CAGTCAGAAGCAAACTGGGCAGCCGAGTTCGCCGAGCTAGAGAAGGAGCGGGACAGCCTG
  GTGAGTGGCGCAGCTCATAGGGAGGAGGAATTATCTGCTCTTCGGAAAGAACTGCAGGAC
  ACTCAGCTCAAACTGGCCAGCACAGAGGAATCTATGTGCCAGCTTGCCAAAGACCAACGA
  AAAATGCTTCTGGTGGGGTCCAGGAAGGCTGCGGAGCAGGTGATACAAGACGCCCTGAAC
  CAGCTTGAAGAACCTCCTCTCATCAGCTGCGCTGGGTCTGCAGATCACCTCCTCTCCACG
  GTCACATCCATTTCCAGCTGCATCGAGCAACTGGAGAAAAGCTGGAGCCAGTATCTGGCC
  TGCCCAGAAGACATCAGTGGACTTCTCCATTCCATAACCCTGCTGGCCCACTTGACCAGC
  GACGCCATTGCTCATGGTGCCACCACCTGCCTCAGAGCCCCACCTGAGCCTGCCGACTCA
  CTGACCGAGGCCTGTAAGCAGTATGGCAGGGAAACCCTCGCCTACCTGGCCTCCCTGGAG
  GAAGAGGGAAGCCTTGAGAATGCCGACAGCACAGCCATGAGGAACTGCCTGAGCAAGATC
  AAGGCCATCGGCGAGGAGCTCCTGCCCAGGGGACTGGACATCAAGCAGGAGGAGCTGGGG
  GACCTGGTGGACAAGGAGATGGCGGCCACTTCAGCTGCTATTGAAACTGCCACGGCCAGA
  ATAGAGGAGATGCTCAGCAAATCCCGAGCAGGAGACACAGGAGTCAAATTGGAGGTGAAT
  GAAAGGATCCTTGGTTGCTGTACCAGCCTCATGCAAGCTATTCAGGTGCTCATCGTGGCC
  TCTAAGGACCTCCAGAGAGAGATTGTGGAGAGCGGCAGGGGTACAGCATCCCCTAAAGAG
  TTTTATGCCAAGAACTCTCGATGGACAGAAGGACTTATCTCAGCCTCCAAGGCTGTGGGC
  TGGGGAGCCACTGTCATGGTGGATGCAGCTGATCTGGTGGTACAAGGCAGAGGGAAATTT
  GAGGAGCTAATGGTGTGTTCTCATGAAATTGCTGCTAGCACAGCCCAGCTTGTGGCTGCA
  TCCAAGGTGAAAGCTGATAAGGACAGCCCCAACCTAGCCCAGCTGCAGCAGGCCTCTCGG
  GGAGTGAACCAGGCCACTGCCGGCGTTGTGGCCTCAACCATTTCCGGCAAATCACAGATC
  GAAGAGACAGACAACATGGACTTCTCAAGCATGACGCTGACACAGATCAAACGCCAAGAG
  ATGGATTCTCAGGTTAGGGTGCTAGAGCTAGAAAATGAATTGCAGAAGGAGCGTCAAAAA
  CTGGGAGAGCTTCGGAAAAAGCACTACGAGCTTGCTGGTGTTGCTGAGGGCTGGGAAGAA
  GGAACAGAGGCATCTCCACCTACACTGCAAGAAGTGGTAACCGAAAAAGAATAG
  
  Clone variation with respect to NM_005338.5
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005338
• Insert Size: 7000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005338.3, NP_005329.2
• RefSeq Size: 6626 bp
• RefSeq ORF: 2745 bp
• Locus ID: 3092
• Cytogenetics: 7q11.23
• Domains: I_LWEQ
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease
• Gene Summary: 'The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.