DTX3 (NM_178502) Human Tagged ORF Clone

CAT#: RC211458

DTX3 (Myc-DDK-tagged)-Human deltex homolog 3 (Drosophila) (DTX3)

Product Images

Western blot validation of overexpression lysate (Cat# LY405894) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211458 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DTX3
• Synonyms: deltex3; RNF154
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211458 representing NM_178502
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTTCGTCCTGTCCAGAATGGCAGCCTGTGGAGGCACCTGCAAGAACAAAGTGACTGTGTCCAAGC
  CCGTGTGGGACTTCCTGAGCAAAGAGACCCCAGCCCGGCTGGCCCGGCTTCGGGAGGAGCACCGTGTGTC
  CATCCTCATAGATGGCGAGACTTCTGACATCTATGTTCTCCAGCTTTCCCCACAGGGTCCTCCCCCGGCC
  CCTCCAAATGGGCTCTACCTAGCCCGGAAGGCTCTCAAGGGGCTGCTAAAAGAGGCAGAGAAAGAGCTGA
  AGAAAGCTCAGAGGCAGGGGGAGCTGATGGGCTGCCTGGCTCTGGGGGGTGGAGGGGAGCACCCTGAGAT
  GCACCGCGCAGGCCCACCCCCTCTCCGAGCAGCCCCACTTCTGCCCCCAGGAGCTCGGGGGCTCCCCCCT
  CCTCCTCCCCCCCTGCCCCCACCTCTTCCTCCTCGCCTTCGGGAGGAGGCAGAAGAGCAGGAGAGCACCT
  GCCCCATCTGTCTGGGGGAGATCCAGAATGCCAAGACATTGGAGAAGTGCCGGCATTCATTCTGCGAGGG
  CTGCATCACCCGGGCTCTGCAGGTGAAAAAGGCCTGCCCCATGTGCGGCCGCTTCTATGGGCAGCTGGTG
  GGCAACCAGCCCCAGAATGGGCGGATGCTGGTCTCTAAGGACGCCACCCTCCTACTGCCCAGCTATGAGA
  AGTACGGCACCATTGTCATCCAGTACGTCTTCCCGCCCGGTGTCCAGGGGGCTGAACACCCAAACCCAGG
  AGTTCGGTATCCTGGCACCACACGGGTGGCCTACCTCCCGGACTGCCCTGAGGGCAACAAGGTGCTGACC
  CTGTTCCGCAAGGCGTTTGACCAGCGTCTCACCTTCACTATCGGCACGTCCATGACCACAGGGAGACCGA
  ATGTCATCACCTGGAACGACATCCACCACAAGACCAGCTGCACAGGGGGACCCCAGCTGTTTGGGTACCC
  AGACCCCACCTACCTGACCCGGGTGCAAGAGGAGCTGAGAGCGAAGGGTATCACAGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211458 representing NM_178502
  Red=Cloning site Green=Tags(s)
  
  MSFVLSRMAACGGTCKNKVTVSKPVWDFLSKETPARLARLREEHRVSILIDGETSDIYVLQLSPQGPPPA
  PPNGLYLARKALKGLLKEAEKELKKAQRQGELMGCLALGGGGEHPEMHRAGPPPLRAAPLLPPGARGLPP
  PPPPLPPPLPPRLREEAEEQESTCPICLGEIQNAKTLEKCRHSFCEGCITRALQVKKACPMCGRFYGQLV
  GNQPQNGRMLVSKDATLLLPSYEKYGTIVIQYVFPPGVQGAEHPNPGVRYPGTTRVAYLPDCPEGNKVLT
  LFRKAFDQRLTFTIGTSMTTGRPNVITWNDIHHKTSCTGGPQLFGYPDPTYLTRVQEELRAKGITDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178502
• ORF Size: 1041 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178502.1, NM_178502.2, NM_178502.3, NP_848597.1
• RefSeq Size: 2029 bp
• RefSeq ORF: 1044 bp
• Locus ID: 196403
• Protein Families: Druggable Genome
• Protein Pathways: Notch signaling pathway
• MW: 37.8 kDa
• Gene Summary: DTX3 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]). [supplied by OMIM, Nov 2009]