RNF34 (NM_194271) Human Tagged ORF Clone

CAT#: RC211608

RNF34 (Myc-DDK-tagged)-Human ring finger protein 34 (RNF34), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY403664) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211608 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNF34
• Synonyms: CARP-1; CARP1; hRFI; RFI; RIF; RIFF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211608 representing NM_194271
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAGGCGGGTGCCACGTCTATGTGGGCTTCGTGCTGTGGGCTGCTGAATGAAGTCATGGGAACTG
  GAGCTGTCAGGGGCCAGCAGTCAGCATTTGCAGGAGCCACCGGTCCATTCAGATTTACACCAAACCCTGA
  GTTTTCCACCTACCCACCAGCAGCTACGGAAGGGCCCAACATAGTTTGTAAAGCCTGTGGGCTTTCATTT
  TCAGTCTTTAGAAAGAAGCATGTTTGCTGTGACTGCAAGAAGGATTTTTGCTCCGTTTGTTCAGTCTTAC
  AAGAAAATCTCCGTAGATGTTCTACTTGTCACTTATTACAAGAGACAGCATTTCAGCGCCCTCAGTTAAT
  GCGACTGAAGGTGAAGGACCTGCGGCAGTATCTCATTCTGAGAAATATACCCATAGATACTTGTCGTGAG
  AAAGAAGACTTGGTGGATCTAGTACTGTGCCATCATGGACTAGGCTCTGAGGACGACATGGACACAAGCA
  GTCTGAATTCTTCAAGGTCCCAGACTTCTAGCTTTTTTACACGTTCGTTTTTTTCAAACTATACAGCCCC
  CTCTGCTACTATGTCTTCGTTTCAGGGAGAGCTTATGGATGGAGACCAAACATCCAGATCTGGAGTGCCG
  GCACAGGTACAAAGTGAAATCACTTCAGCAAACACAGAAGATGATGATGACGACGATGATGAGGATGATG
  ATGATGAAGAAGAAAACGCAGAGGATCGGAACCCCGGGCTCTCCAAGGAGAGAGTGAGAGCTTCACTGTC
  TGACTTGTCAAGCCTTGATGATGTGGAAGGAATGAGCGTGCGCCAGCTGAAGGAAATTCTGGCTCGGAAT
  TTTGTCAACTATTCTGGCTGTTGTGAAAAATGGGAACTGGTAGAGAAAGTAAACCGGTTATACAAAGAGA
  ATGAAGAAAACCAAAAGTCCTATGGCGAGCGGCTGCAGCTGCAGGATGAGGAAGACGACAGCCTGTGTCG
  CATCTGCATGGATGCCGTCATCGACTGTGTCCTACTGGAGTGTGGGCACATGGTTACCTGCACCAAGTGC
  GGCAAGCGCATGAGTGAGTGTCCCATCTGCCGGCAGTATGTGGTGCGAGCCGTGCACGTGTTCAAGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211608 representing NM_194271
  Red=Cloning site Green=Tags(s)
  
  MRKAGATSMWASCCGLLNEVMGTGAVRGQQSAFAGATGPFRFTPNPEFSTYPPAATEGPNIVCKACGLSF
  SVFRKKHVCCDCKKDFCSVCSVLQENLRRCSTCHLLQETAFQRPQLMRLKVKDLRQYLILRNIPIDTCRE
  KEDLVDLVLCHHGLGSEDDMDTSSLNSSRSQTSSFFTRSFFSNYTAPSATMSSFQGELMDGDQTSRSGVP
  AQVQSEITSANTEDDDDDDDEDDDDEEENAEDRNPGLSKERVRASLSDLSSLDDVEGMSVRQLKEILARN
  FVNYSGCCEKWELVEKVNRLYKENEENQKSYGERLQLQDEEDDSLCRICMDAVIDCVLLECGHMVTCTKC
  GKRMSECPICRQYVVRAVHVFKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194271
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194271.1, NM_194271.2, NP_919247.1
• RefSeq Size: 2105 bp
• RefSeq ORF: 1122 bp
• Locus ID: 80196
• Protein Families: Druggable Genome
• MW: 41.6 kDa
• Gene Summary: The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]