PKC mu (PRKD1) (NM_002742) Human Tagged ORF Clone

CAT#: RC211676

PRKD1 (Myc-DDK-tagged)-Human protein kinase D1 (PRKD1)

Product Images

Western blot validation of overexpression lysate (Cat# LY419136) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211676 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PRKD1 protein (Cat# TP311676). The protein was produced from HEK293T cells transfected with PRKD1 cDNA clone (Cat# RC211676) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRKD1
• Synonyms: CHDED; PKC-MU; PKCM; PKD; PRKCM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211676 representing NM_002742
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCCCCTCCGGTCCTGCGGCCGCCCAGTCCGCTGCTGCCCGTGGCGGCGGCAGCTGCCGCAGCGG
  CCGCCGCACTGGTCCCAGGGTCCGGGCCCGGGCCCGCGCCGTTCTTGGCTCCTGTCGCGGCCCCGGTCGG
  GGGCATCTCGTTCCATCTGCAGATCGGCCTGAGCCGTGAGCCGGTGCTGCTGCTGCAGGACTCGTCCGGG
  GACTACAGCCTGGCGCACGTCCGCGAGATGGCTTGCTCCATTGTCGACCAGAAGTTCCCTGAATGTGGTT
  TCTACGGAATGTATGATAAGATCCTGCTTTTTCGCCATGACCCTACCTCTGAAAACATCCTTCAGCTGGT
  GAAAGCGGCCAGTGATATCCAGGAAGGCGATCTTATTGAAGTGGTCTTGTCAGCTTCCGCCACCTTTGAA
  GACTTTCAGATTCGTCCCCACGCTCTCTTTGTTCATTCATACAGAGCTCCAGCTTTCTGTGATCACTGTG
  GAGAAATGCTGTGGGGGCTGGTACGTCAAGGTCTTAAATGTGAAGGGTGTGGTCTGAATTACCATAAGAG
  ATGTGCATTTAAAATACCCAACAATTGCAGCGGTGTGAGGCGGAGAAGGCTCTCAAACGTTTCCCTCACT
  GGGGTCAGCACCATCCGCACATCATCTGCTGAACTCTCTACAAGTGCCCCTGATGAGCCCCTTCTGCAAA
  AATCACCATCAGAGTCGTTTATTGGTCGAGAGAAGAGGTCAAATTCTCAATCATACATTGGACGACCAAT
  TCACCTTGACAAGATTTTGATGTCTAAAGTTAAAGTGCCGCACACATTTGTCATCCACTCCTACACCCGG
  CCCACAGTGTGCCAGTACTGCAAGAAGCTTCTGAAGGGGCTTTTCAGGCAGGGCTTGCAGTGCAAAGATT
  GCAGATTCAACTGCCATAAACGTTGTGCACCGAAAGTACCAAACAACTGCCTTGGCGAAGTGACCATTAA
  TGGAGATTTGCTTAGCCCTGGGGCAGAGTCTGATGTGGTCATGGAAGAAGGGAGTGATGACAATGATAGT
  GAAAGGAACAGTGGGCTCATGGATGATATGGAAGAAGCAATGGTCCAAGATGCAGAGATGGCAATGGCAG
  AGTGCCAGAACGACAGTGGCGAGATGCAAGATCCAGACCCAGACCACGAGGACGCCAACAGAACCATCAG
  TCCATCAACAAGCAACAATATCCCACTCATGAGGGTAGTGCAGTCTGTCAAACACACGAAGAGGAAAAGC
  AGCACAGTCATGAAAGAAGGATGGATGGTCCACTACACCAGCAAGGACACGCTGCGGAAACGGCACTATT
  GGAGATTGGATAGCAAATGTATTACCCTCTTTCAGAATGACACAGGAAGCAGGTACTACAAGGAAATTCC
  TTTATCTGAAATTTTGTCTCTGGAACCAGTAAAAACTTCAGCTTTAATTCCTAATGGGGCCAATCCTCAT
  TGTTTCGAAATCACTACGGCAAATGTAGTGTATTATGTGGGAGAAAATGTGGTCAATCCTTCCAGCCCAT
  CACCAAATAACAGTGTTCTCACCAGTGGCGTTGGTGCAGATGTGGCCAGGATGTGGGAGATAGCCATCCA
  GCATGCCCTTATGCCCGTCATTCCCAAGGGCTCCTCCGTGGGTACAGGAACCAACTTGCACAGAGATATC
  TCTGTGAGTATTTCAGTATCAAATTGCCAGATTCAAGAAAATGTGGACATCAGCACAGTATATCAGATTT
  TTCCTGATGAAGTACTGGGTTCTGGACAGTTTGGAATTGTTTATGGAGGAAAACATCGTAAAACAGGAAG
  AGATGTAGCTATTAAAATCATTGACAAATTACGATTTCCAACAAAACAAGAAAGCCAGCTTCGTAATGAG
  GTTGCAATTCTACAGAACCTTCATCACCCTGGTGTTGTAAATTTGGAGTGTATGTTTGAGACGCCTGAAA
  GAGTGTTTGTTGTTATGGAAAAACTCCATGGAGACATGCTGGAAATGATCTTGTCAAGTGAAAAGGGCAG
  GTTGCCAGAGCACATAACGAAGTTTTTAATTACTCAGATACTCGTGGCTTTGCGGCACCTTCATTTTAAA
  AATATCGTTCACTGTGACCTCAAACCAGAAAATGTGTTGCTAGCCTCAGCTGATCCTTTTCCTCAGGTGA
  AACTTTGTGATTTTGGTTTTGCCCGGATCATCGGAGAGAAGTCTTTCCGGAGGTCAGTGGTGGGTACCCC
  CGCTTACCTGGCTCCTGAGGTCCTAAGGAACAAGGGCTACAATCGCTCTCTAGACATGTGGTCTGTTGGG
  GTCATCATCTATGTAAGCCTAAGCGGCACATTCCCATTTAATGAAGATGAAGACATACACGACCAAATTC
  AGAATGCAGCTTTCATGTATCCACCAAATCCCTGGAAGGAAATATCTCATGAAGCCATTGATCTTATCAA
  CAATTTGCTGCAAGTAAAAATGAGAAAGCGCTACAGTGTGGATAAGACCTTGAGCCACCCTTGGCTACAG
  GACTATCAGACCTGGTTAGATTTGCGAGAGCTGGAATGCAAAATCGGGGAGCGCTACATCACCCATGAAA
  GTGATGACCTGAGGTGGGAGAAGTATGCAGGCGAGCAGGGGCTGCAGTACCCCACACACCTGATCAATCC
  AAGTGCTAGCCACAGTGACACTCCTGAGACTGAAGAAACAGAAATGAAAGCCCTCGGTGAGCGTGTCAGC
  ATCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211676 representing NM_002742
  Red=Cloning site Green=Tags(s)
  
  MSAPPVLRPPSPLLPVAAAAAAAAAALVPGSGPGPAPFLAPVAAPVGGISFHLQIGLSREPVLLLQDSSG
  DYSLAHVREMACSIVDQKFPECGFYGMYDKILLFRHDPTSENILQLVKAASDIQEGDLIEVVLSASATFE
  DFQIRPHALFVHSYRAPAFCDHCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRRRRLSNVSLT
  GVSTIRTSSAELSTSAPDEPLLQKSPSESFIGREKRSNSQSYIGRPIHLDKILMSKVKVPHTFVIHSYTR
  PTVCQYCKKLLKGLFRQGLQCKDCRFNCHKRCAPKVPNNCLGEVTINGDLLSPGAESDVVMEEGSDDNDS
  ERNSGLMDDMEEAMVQDAEMAMAECQNDSGEMQDPDPDHEDANRTISPSTSNNIPLMRVVQSVKHTKRKS
  STVMKEGWMVHYTSKDTLRKRHYWRLDSKCITLFQNDTGSRYYKEIPLSEILSLEPVKTSALIPNGANPH
  CFEITTANVVYYVGENVVNPSSPSPNNSVLTSGVGADVARMWEIAIQHALMPVIPKGSSVGTGTNLHRDI
  SVSISVSNCQIQENVDISTVYQIFPDEVLGSGQFGIVYGGKHRKTGRDVAIKIIDKLRFPTKQESQLRNE
  VAILQNLHHPGVVNLECMFETPERVFVVMEKLHGDMLEMILSSEKGRLPEHITKFLITQILVALRHLHFK
  NIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRNKGYNRSLDMWSVG
  VIIYVSLSGTFPFNEDEDIHDQIQNAAFMYPPNPWKEISHEAIDLINNLLQVKMRKRYSVDKTLSHPWLQ
  DYQTWLDLRELECKIGERYITHESDDLRWEKYAGEQGLQYPTHLINPSASHSDTPETEETEMKALGERVS
  IL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002742
• ORF Size: 2736 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002742.1, NM_002742.2, NP_002733.1
• RefSeq Size: 3742 bp
• RefSeq ORF: 2739 bp
• Locus ID: 5587
• Cytogenetics: 14q12
• Domains: pkinase, TyrKc, PH, DAG_PE-bind, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 101.5 kDa
• Gene Summary: 'The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion. [provided by RefSeq, Jan 2017]'