PKC mu (PRKD1) (NM_002742) Human Untagged Clone

CAT#: SC118457

PRKD1 (untagged)-Human protein kinase D1 (PRKD1)


  "NM_002742" in other vectors (7)

Reconstitution Protocol

USD 1,840.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PRKD1
Synonyms CHDED; PKC-MU; PKCM; PKD; PRKCM
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_002742 edited
ATGAGCGCCCCTCCGGTCCTGCGGCCGCCCAGTCCGCTGCTGCCCGTGGCGGCGGCAGCT
GCCGCAGCGGCCGCCGCACTGGTCCCAGGGTCCGGGCCCGGGCCCGCGCCGTTCTTGGCT
CCTGTCGCGGCCCCGGTCGGGGGCATCTCGTTCCATCTGCAGATCGGCCTGAGCCGTGAG
CCGGTGCTGCTGCTGCAGGACTCGTCCGGGGACTACAGCCTGGCGCACGTCCGCGAGATG
GCTTGCTCCATTGTCGACCAGAAGTTCCCTGAATGTGGTTTCTACGGAATGTATGATAAG
ATCCTGCTTTTTCGCCATGACCCTACCTCTGAAAACATCCTTCAGCTGGTGAAAGCGGCC
AGTGATATCCAGGAAGGCGATCTTATTGAAGTGGTCTTGTCAGCTTCCGCCACCTTTGAA
GACTTTCAGATTCGTCCCCACGCTCTCTTTGTTCATTCATACAGAGCTCCAGCTTTCTGT
GATCACTGTGGAGAAATGCTGTGGGGGCTGGTACGTCAAGGTCTTAAATGTGAAGGGTGT
GGTCTGAATTACCATAAGAGATGTGCATTTAAAATACCCAACAATTGCAGCGGTGTGAGG
CGGAGAAGGCTCTCAAACGTTTCCCTCACTGGGGTCAGCACCATCCGCACATCATCTGCT
GAACTCTCTACAAGTGCCCCTGATGAGCCCCTTCTGCAAAAATCACCATCAGAGTCGTTT
ATTGGTCGAGAGAAGAGGTCAAATTCTCAATCATACATTGGACGACCAATTCACCTTGAC
AAGATTTTGATGTCTAAAGTTAAAGTGCCGCACACATTTGTCATCCACTCCTACACCCGG
CCCACAGTGTGCCAGTACTGCAAGAAGCTTCTGAAGGGGCTTTTCAGGCAGGGCTTGCAG
TGCAAAGATTGCAGATTCAACTGCCATAAACGTTGTGCACCGAAAGTACCAAACAACTGC
CTTGGCGAAGTGACCATTAATGGAGATTTGCTTAGCCCTGGGGCAGAGTCTGATGTGGTC
ATGGAAGAAGGGAGTGATGACAATGATAGTGAAAGGAACAGTGGGCTCATGGATGATATG
GAAGAAGCAATGGTCCAAGATGCAGAGATGGCAATGGCAGAGTGCCAGAACGACAGTGGC
GAGATGCAAGATCCAGACCCAGACCACGAGGACGCCAACAGAACCATCAGTCCATCAACA
AGCAACAATATCCCACTCATGAGGGTAGTGCAGTCTGTCAAACACACGAAGAGGAAAAGC
AGCACAGTCATGAAAGAAGGATGGATGGTCCACTACACCAGCAAGGACACGCTGCGGAAA
CGGCACTATTGGAGATTGGATAGCAAATGTATTACCCTCTTTCAGAATGACACAGGAAGC
AGGTACTACAAGGAAATTCCTTTATCTGAAATTTTGTCTCTGGAACCAGTAAAAACTTCA
GCTTTAATTCCTAATGGGGCCAATCCTCATTGTTTCGAAATCACTACGGCAAATGTAGTG
TATTATGTGGGAGAAAATGTGGTCAATCCTTCCAGCCCATCACCAAATAACAGTGTTCTC
ACCAGTGGCGTTGGTGCAGATGTGGCCAGGATGTGGGAGATAGCCATCCAGCATGCCCTT
ATGCCCGTCATTCCCAAGGGCTCCTCCGTGGGTACAGGAACCAACTTGCACAGAGATATC
TCTGTGAGTATTTCAGTATCAAATTGCCAGATTCAAGAAAATGTGGACATCAGCACAGTA
TATCAGATTTTTCCTGATGAAGTACTGGGTTCTGGACAGTTTGGAATTGTTTATGGAGGA
AAACATCGTAAAACAGGAAGAGATGTAGCTATTAAAATCATTGACAAATTACGATTTCCA
ACAAAACAAGAAAGCCAGCTTCGTAATGAGGTTGCAATTCTACAGAACCTTCATCACCCT
GGTGTTGTAAATTTGGAGTGTATGTTTGAGACGCCTGAAAGAGTGTTTGTTGTTATGGAA
AAACTCCATGGAGACATGCTGGAAATGATCTTGTCAAGTGAAAAGGGCAGGTTGCCAGAG
CACATAACGAAGTTTTTAATTACTCAGATACTCGTGGCTTTGCGGCACCTTCATTTTAAA
AATATCGTTCACTGTGACCTCAAACCAGAAAATGTGTTGCTAGCCTCAGCTGATCCTTTT
CCTCAGGTGAAACTTTGTGATTTTGGTTTTGCCCGGATCATCGGAGAGAAGTCTTTCCGG
AGGTCAGTGGTGGGTACCCCCGCTTACCTGGCTCCTGAGGTCCTAAGGAACAAGGGCTAC
AATCGCTCTCTAGACATGTGGTCTGTTGGGGTCATCATCTATGTAAGCCTAAGCGGCACA
TTCCCATTTAATGAAGATGAAGACATACACGACCAAATTCAGAATGCAGCTTTCATGTAT
CCACCAAATCCCTGGAAGGAAATATCTCATGAAGCCATTGATCTTATCAACAATTTGCTG
CAAGTAAAAATGAGAAAGCGCTACAGTGTGGATAAGACCTTGAGCCACCCTTGGCTACAG
GACTATCAGACCTGGTTAGATTTGCGAGAGCTGGAATGCAAAATCGGGGAGCGCTACATC
ACCCATGAAAGTGATGACCTGAGGTGGGAGAAGTATGCAGGCGAGCAGGGGCTGCAGTAC
CCCACACACCTGATCAATCCAAGTGCTAGCCACAGTGACACTCCTGAGACTGAAGAAACA
GAAATGAAAGCCCTCGGTGAGCGTGTCAGCATCCTCTGA
>OriGene 5' read for NM_002742 unedited
ATTTACANATTTGTATACACTCACTATAGGCGGCCGCGAATCGGCACGAGTCCGGAAAGT
TTTTATTTTCCGTCTCGGGCTCTCGGNAGAAAGAAGCTCCTGGCTCAGCGGCTGCAAAAC
TTTCCTGACCGCCGCGCCGCCAGCCCCCGCCCTCCGCTGCCCGGCCCTGCGCCCCGCCGA
GCGATGAGCGCCCCTCCGGTCCTGCGGCCGCCCAGTCCGCTTGCTGCCCGTGGCGGCGGC
AGCTGCCGCAGCGGCCGCCGCACTGGTCCCAGGGTCCGGGCCCGGGCCCGCGCCGTTCTT
GGCTCCTGTCGCGGCCCCGGTCGGGGGCATCTCGTTCCATCTGCAGATCGGCCTGAGCCG
TGAGCCGGTGCTGCTGCTGCAGGACTCGTCCGGGGACTACAGCCTGGCGCACGTCCGCGA
GATGGCTTGCTCCATTGTCGACCAGAAGTTCCCTGAATGTGGTTTCTACGGAATGTATGA
TAAGATCCTGCTTTTTCGCCATGACCCTACCTCTGAAAACATCCTTCAGCTGGTGAAAGC
GGCCAGTGATATCCAGGAAGGCGATCTTATTGAAGTGGTCTTGTCAGCTTCCGCCACCCT
TTGAAGACTTTCAGATTCGTCCCCACGCTCTCTTTGTTCATTCATACAGAGCTCCAGCTT
TCTGTGATCACTGCGGAGAAAAGCTGTGCGGGCTGGTACGTCAAAGTCCTAAATGTGAAG
GGTGTGGTCTGAAATACCATAAGAGATGTGCATTTAAAAACCCAACAATTGCAACGGGTG
TGAAGCCGGAGAGGGCTCTCAAACGCTTCCCTCACTGGGGGCAGAACCATCCGCACATAA
TTTGCTGAAATCTCTAAAACTGCCCCCGAGGAGCCCCTTCTGCAAAAATAACCACACAAG
CCGTTTATTGGTCCAGAGAGAAGGCCAAATTCTCATCATC
>OriGene 3' read for NM_002742 unedited
CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGACCAAAATATTTTTTAT
TATTGAAAATACACAAAGGTGAAAGGTTGCTGAGCAGCTGATTTTCCATAGTACAACAGA
GTATTATAAGAAAGAGGGAAATGTATCTGTTATATATGGCAGTTTACATTTCTAAGGCAT
TTAGGCTTAAGAGCACTAGGTCTTGCACAGCTAGGTGCATTGCCTTGAGCCCAAATATAC
TGAGGCCCACCAGCCAGGCACAGGCATCAAACCTCCACCTTAAACCTTGATCTTGCCTTC
GTCCCTCCCGACCTAATCCCCCTATCCTTTCCCCCCTTCGCTCCGCCTTTGGCCCTAGCT
ACCCCCCCTCCCCCCTTTCTTCTTCCCTTCCCTCCACCACTTTCCCCCCCCCCCCCCTCC
TGGAACATCCTCCCCTTCACCTCCTCTCCTCACCCCTCTACCCCTTCCCTCTCTCCTCCC
CCCCCCCCTTCCCCCCCCCTCTCTCCCTCCCACCCTCCCACACCTTTTCCCGCTCTCCCT
CCCCCCTCTCCCATCCTCCTCTATCTCCCCTCTCAAACCCCCTTTTTCCCCACCCCTCCC
CCCTTCCCTTCCTCTCACCCCCCCGTACGCCTATATTTACTTCCTCCCCTTCCTCCCCCC
CCCTTATTCCCTTCTTTCCCACACTTATTCATTCCCCGCGACACCCGCTTCCCCCTCCTC
CCCCACCGCCCTTCCGTTATAGCTTGCCCCCCCACACTCTCCCCATTCCCCACCCCTCGC
CCCCCATCTCCCCCTTTCCTCCCTCCCCTTATCCCTTTCCCTCCCTCACACGCCCCACCT
CATCCCTCCCACCCCCCCACCTCCCACCCCATCCATTATCCCCGTCTCCCCCCCATCTAT
CCCCTCTCCCCCTCTCCTTTCTTATCCTCCTCCCCCCCTTTTCTTATTTTTTTTCCCTCC
CCGCCCCCACCCCTTCTCGTCCCATCCCCCCTCTTATACATCCCCGGTTA
Restriction Sites NotI-NotI     
ACCN NM_002742
Insert Size 3570 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002742.1, NP_002733.1
RefSeq Size 3742 bp
RefSeq ORF 2739 bp
Locus ID 5587
Cytogenetics 14q12
Domains pkinase, TyrKc, PH, DAG_PE-bind, S_TKc
Protein Families Druggable Genome, Protein Kinase
Gene Summary 'The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion. [provided by RefSeq, Jan 2017]'
Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 2.

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