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Collagen IV (COL4A4) (NM_000092) Human Tagged ORF Clone
CAT#: RC211841
- TrueORF®
COL4A4 (Myc-DDK-tagged)-Human collagen, type IV, alpha 4 (COL4A4)
"NM_000092" in other vectors (2)
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | COL4A4 |
| Synonyms | ATS2; BFH; CA44 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211841 representing NM_000092
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGTCTCTGCACATAGTACTAATGAGGTGCTCCTTCAGATTGACCAAGTCCTTGGCCACAGGTCCCT GGTCACTTATACTCATTCTCTTTTCTGTACAATATGTATATGGGAGTGGAAAGAAATACATTGGTCCTTG TGGAGGAAGAGATTGCTCTGTTTGCCACTGTGTTCCTGAAAAGGGGTCTCGGGGTCCACCAGGACCACCA GGGCCACAGGGTCCAATTGGACCCCTGGGAGCCCCAGGACCCATTGGGCTTTCAGGAGAGAAAGGAATGA GAGGGGACCGCGGCCCTCCTGGAGCAGCAGGGGACAAAGGAGATAAGGGTCCAACTGGTGTTCCTGGATT TCCAGGTTTAGATGGCATACCTGGGCACCCAGGGCCTCCTGGACCCAGAGGCAAACCTGGTATGAGTGGC CACAATGGCTCAAGAGGTGACCCAGGGTTTCCAGGAGGAAGAGGAGCTCTTGGCCCAGGAGGCCCCCTAG GCCATCCTGGGGAAAAGGGAGAAAAAGGAAATTCAGTGTTCATTTTAGGTGCCGTTAAAGGTATTCAGGG AGACAGAGGGGACCCAGGACTGCCTGGCTTACCAGGATCTTGGGGTGCAGGAGGACCGGCAGGTCCCACA GGATATCCTGGAGAGCCAGGGTTAGTGGGACCTCCGGGCCAACCAGGGCGTCCAGGTTTGAAGGGAAATC CCGGTGTGGGAGTAAAGGGGCAAATGGGAGACCCGGGTGAGGTTGGTCAGCAAGGTTCTCCTGGACCCAC CCTGTTGGTAGAGCCACCTGACTTTTGTCTCTATAAAGGAGAAAAGGGTATAAAAGGAATTCCTGGAATG GTTGGACTGCCAGGACCACCAGGACGCAAGGGAGAATCTGGTATTGGGGCAAAAGGAGAAAAAGGTATTC CTGGATTTCCAGGGCCTCGGGGGGATCCTGGTTCCTATGGATCTCCAGGTTTTCCAGGATTAAAGGGAGA ACTAGGACTGGTTGGAGATCCTGGGCTATTTGGATTAATTGGCCCAAAGGGGGATCCTGGAAATCGAGGG CACCCAGGACCACCAGGTGTTTTGGTGACTCCACCTCTTCCACTCAAAGGCCCACCAGGGGACCCAGGGT TCCCTGGCCGCTATGGAGAAACAGGGGATGTTGGACCACCTGGTCCCCCAGGTCTCTTGGGCAGACCAGG GGAAGCCTGTGCAGGCATGATAGGACCCCCTGGGCCACAAGGATTTCCTGGTCTTCCTGGGCTTCCAGGA GAAGCTGGTATTCCTGGGAGACCTGATTCTGCTCCAGGAAAACCAGGGAAGCCAGGATCACCTGGCTTGC CTGGAGCACCAGGCCTGCAGGGCCTCCCAGGATCAAGTGTGATATACTGTAGTGTTGGGAACCCCGGACC ACAAGGAATAAAAGGCAAAGTTGGTCCCCCAGGAGGAAGAGGCCCAAAAGGAGAAAAAGGAAATGAAGGA CTCTGTGCCTGTGAGCCTGGACCCATGGGCCCCCCTGGCCCTCCAGGACTTCCTGGGAGGCAGGGGAGTA AGGGAGACTTGGGGCTCCCTGGCTGGCTTGGAACAAAAGGTGACCCAGGACCTCCTGGTGCTGAAGGACC TCCAGGGCTACCAGGAAAGCATGGTGCCTCTGGACCACCTGGCAACAAAGGGGCGAAGGGTGACATGGTT GTATCAAGAGTTAAAGGGCACAAAGGAGAAAGAGGTCCTGATGGGCCCCCAGGATTTCCAGGGCAGCCAG GATCACATGGTCGGGATGGACATGCTGGAGAAAAAGGGGATCCAGGACCTCCAGGGGATCATGAAGATGC GACCCCAGGTGGTAAAGGATTTCCTGGACCTCTGGGCCCCCCAGGCAAAGCAGGACCTGTGGGGCCCCCA GGACTGGGATTTCCTGGTCCACCAGGAGAGCGAGGCCACCCAGGAGTTCCAGGCCACCCAGGTGTGAGGG GCCCTGATGGCTTGAAGGGTCAGAAAGGTGACACAATTTCTTGCAACGTAACCTACCCTGGGAGGCATGG CCCTCCAGGTTTTGATGGACCTCCAGGTCCGAAGGGATTTCCAGGTCCCCAAGGTGCCCCTGGGCTGAGT GGTTCAGATGGGCATAAAGGCAGACCTGGCACACCAGGAACAGCGGAAATACCAGGTCCACCTGGTTTTC GTGGTGACATGGGAGATCCGGGTTTTGGAGGTGAAAAGGGGTCCTCCCCTGTTGGGCCCCCAGGCCCTCC CGGCTCACCAGGAGTGAATGGTCAGAAAGGAATCCCGGGAGACCCTGCATTTGGTCACCTGGGACCCCCG GGAAAGAGGGGTCTTTCAGGAGTGCCAGGGATAAAAGGACCCAGAGGTGATCCGGGATGTCCAGGGGCTG AAGGGCCAGCTGGCATTCCTGGATTCCTAGGTCTCAAAGGTCCCAAAGGCAGAGAGGGACATGCTGGGTT TCCAGGTGTCCCAGGTCCACCTGGCCATTCCTGTGAAAGAGGTGCTCCAGGGATACCAGGGCAACCGGGA CTCCCTGGGTATCCAGGTAGCCCAGGTGCTCCAGGTGGGAAAGGACAGCCGGGAGATGTGGGGCCTCCCG GGCCAGCTGGAATGAAAGGCCTCCCCGGACTCCCAGGACGGCCTGGGGCACATGGTCCCCCAGGCCTCCC AGGAATCCCAGGTCCCTTTGGAGATGATGGGCTACCTGGTCCTCCAGGTCCAAAGGGACCCCGGGGGCTG CCTGGTTTCCCAGGTTTTCCCGGAGAAAGAGGAAAGCCTGGTGCAGAGGGATGTCCTGGCGCAAAGGGAG AACCTGGAGAGAAGGGCATGTCTGGCCTTCCTGGAGACCGGGGACTGAGAGGGGCCAAAGGAGCCATAGG ACCTCCCGGAGATGAAGGAGAAATGGCTATCATTTCACAAAAGGGAACACCTGGGGAACCTGGACCTCCT GGAGATGATGGATTCCCAGGAGAAAGAGGTGATAAAGGAACTCCCGGGATGCAAGGGAGAAGAGGAGAGC CGGGAAGATACGGACCACCTGGATTTCACAGAGGGGAACCTGGTGAGAAAGGTCAGCCAGGGCCTCCTGG ACCCCCAGGCCCTCCAGGCTCAACTGGTCTAAGAGGGTTCATTGGTTTTCCAGGACTTCCAGGTGACCAG GGTGAGCCAGGTTCTCCAGGTCCCCCTGGATTTTCAGGAATTGATGGAGCAAGAGGACCTAAAGGAAACA AAGGTGACCCTGCCAGTCACTTTGGTCCACCTGGTCCAAAGGGTGAGCCAGGTAGCCCTGGATGTCCAGG GCATTTTGGAGCATCCGGAGAGCAGGGCTTGCCTGGTATTCAAGGGCCCAGAGGATCACCTGGAAGGCCA GGGCCACCTGGCTCCTCTGGACCACCAGGGTGCCCAGGTGATCACGGGATGCCTGGGCTGAGGGGACAGC CAGGAGAAATGGGAGACCCTGGGCCAAGAGGCCTCCAGGGGGATCCAGGGATACCAGGTCCTCCGGGAAT AAAAGGTCCCTCCGGATCACCTGGCCTGAACGGCTTGCATGGATTGAAAGGTCAGAAAGGAACTAAAGGT GCTTCAGGTTTGCATGATGTGGGGCCACCTGGTCCAGTGGGAATACCTGGGCTAAAAGGGGAGAGAGGAG ACCCTGGGAGCCCAGGAATCTCTCCTCCAGGTCCTCGTGGAAAGAAAGGTCCCCCAGGACCCCCAGGGAG TTCAGGACCACCTGGTCCTGCAGGTGCCACAGGAAGAGCTCCTAAGGACATTCCTGACCCGGGTCCACCT GGAGATCAGGGACCTCCTGGTCCTGATGGCCCAAGAGGAGCACCTGGGCCTCCAGGCCTCCCTGGGAGTG TTGACCTTCTGAGAGGGGAGCCAGGTGACTGTGGTCTACCAGGGCCACCAGGTCCCCCTGGCCCACCAGG CCCTCCAGGATACAAAGGCTTTCCAGGATGTGATGGAAAAGATGGCCAGAAAGGACCAGTGGGATTCCCG GGACCGCAGGGACCACATGGATTTCCTGGGCCACCTGGAGAGAAGGGTTTACCTGGACCTCCAGGGAGAA AAGGGCCCACTGGTCTTCCGGGTCCCAGAGGTGAACCGGGGCCACCTGCAGATGTGGATGACTGTCCCCG AATCCCAGGCCTTCCTGGGGCGCCAGGCATGAGAGGACCAGAAGGAGCCATGGGGCTCCCTGGAATGAGA GGCCCCTCAGGACCAGGGTGCAAAGGAGAGCCTGGGCTGGATGGCAGGAGGGGTGTGGATGGCGTCCCTG GGTCTCCTGGGCCTCCCGGACGTAAAGGTGACACAGGAGAAGACGGCTACCCTGGAGGACCAGGGCCTCC TGGTCCCATTGGGGATCCTGGGCCCAAAGGGTTTGGCCCTGGATACCTCGGTGGCTTCCTCCTGGTTCTC CACAGTCAGACGGACCAGGAGCCCACCTGCCCCCTGGGCATGCCCAGGCTCTGGACTGGGTATAGTCTGT TATACCTGGAAGGGCAAGAGAAAGCTCACAATCAAGACCTTGGTCTGGCAGGGTCTTGCCTTCCCGTATT TAGCACGCTGCCCTTTGCCTACTGCAACATCCACCAGGTGTGCCACTATGCCCAGAGAAACGACAGATCC TACTGGCTGGCCAGCGCTGCGCCCCTCCCCATGATGCCACTCTCTGAAGAGGCGATCCGCCCCTATGTCA GCCGCTGTGCGGTATGCGAGGCCCCGGCCCAGGCGGTGGCGGTGCACAGCCAGGACCAGTCCATCCCCCC ATGTCCGCAGACCTGGAGGAGCCTCTGGATCGGGTATTCATTCCTGATGCACACAGGAGCTGGGGACCAA GGAGGAGGGCAGGCCCTTATGTCACCTGGCAGCTGCCTGGAAGATTTCAGAGCAGCACCATTCCTTGAAT GCCAGGGCCGGCAGGGAACTTGCCACTTTTTCGCAAATAAGTATAGCTTCTGGCTCACAACGGTGAAAGC AGACTTGCAGTTTTCCTCTGCTCCAGCACCAGACACCTTAAAAGAAAGCCAGGCCCAACGCCAGAAAATC AGCCGGTGCCAGGTCTGCGTGAAGTATAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211841 representing NM_000092
Red=Cloning site Green=Tags(s) MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPP GPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSG HNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPT GYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGM VGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRG HPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPG EAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEG LCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMV VSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPP GLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLS GSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPP GKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPG LPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGL PGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPP GDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQ GEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRP GPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKG ASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPP GDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFP GPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMR GPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVL HSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRS YWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQ GGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKI SRCQVCVKYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000092 |
| ORF Size | 5070 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000092.1, NM_000092.2, NM_000092.3, NM_000092.4, NP_000083.2, NP_000083.3 |
| RefSeq Size | 7843 bp |
| RefSeq ORF | 5073 bp |
| Locus ID | 1286 |
| Cytogenetics | 2q36.3 |
| Protein Families | Druggable Genome |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer |
| MW | 164.04 kDa |
| Gene Summary | 'This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR. [provided by RefSeq, Jul 2008]' |
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