Collagen IV (COL4A4) (NM_000092) Human Untagged Clone

CAT#: SC300009

COL4A4 (untagged)-Human collagen, type IV, alpha 4 (COL4A4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL4A4
• Synonyms: ATS2; BFH; CA44
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000092, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGTCTCTGCACATAGTACTAATGAGGTGCTCCTTCAGATTGACCAAGTCCTTGGCCACAGGTCCCT
  GGTCACTTATACTCATTCTCTTTTCTGTACAATATGTATATGGGAGTGGAAAGAAATACATTGGTCCTTG
  TGGAGGAAGAGATTGCTCTGTTTGCCACTGTGTTCCTGAAAAGGGGTCTCGGGGTCCACCAGGACCACCA
  GGGCCACAGGGTCCAATTGGACCCCTGGGAGCCCCAGGACCCATTGGGCTTTCAGGAGAGAAAGGAATGA
  GAGGGGACCGCGGCCCTCCTGGAGCAGCAGGGGACAAAGGAGATAAGGGTCCAACTGGTGTTCCTGGATT
  TCCAGGTTTAGATGGCATACCTGGGCACCCAGGGCCTCCTGGACCCAGAGGCAAACCTGGTATGAGTGGC
  CACAATGGCTCAAGAGGTGACCCAGGGTTTCCAGGAGGAAGAGGAGCTCTTGGCCCAGGAGGCCCCCTAG
  GCCATCCTGGGGAAAAGGGAGAAAAAGGAAATTCAGTGTTCATTTTAGGTGCCGTTAAAGGTATTCAGGG
  AGACAGAGGGGACCCAGGACTGCCTGGCTTACCAGGATCTTGGGGTGCAGGAGGACCGGCAGGTCCCACA
  GGATATCCTGGAGAGCCAGGGTTAGTGGGACCTCCGGGCCAACCAGGGCGTCCAGGTTTGAAGGGAAATC
  CCGGTGTGGGAGTAAAGGGGCAAATGGGAGACCCGGGTGAGGTTGGTCAGCAAGGTTCTCCTGGACCCAC
  CCTGTTGGTAGAGCCACCTGACTTTTGTCTCTATAAAGGAGAAAAGGGTATAAAAGGAATTCCTGGAATG
  GTTGGACTGCCAGGACCACCAGGACGCAAGGGAGAATCTGGTATTGGGGCAAAAGGAGAAAAAGGTATTC
  CTGGATTTCCAGGGCCTCGGGGGGATCCTGGTTCCTATGGATCTCCAGGTTTTCCAGGATTAAAGGGAGA
  ACTAGGACTGGTTGGAGATCCTGGGCTATTTGGATTAATTGGCCCAAAGGGGGATCCTGGAAATCGAGGG
  CACCCAGGACCACCAGGTGTTTTGGTGACTCCACCTCTTCCACTCAAAGGCCCACCAGGGGACCCAGGGT
  TCCCTGGCCGCTATGGAGAAACAGGGGATGTTGGACCACCTGGTCCCCCAGGTCTCTTGGGCAGACCAGG
  GGAAGCCTGTGCAGGCATGATAGGACCCCCTGGGCCACAAGGATTTCCTGGTCTTCCTGGGCTTCCAGGA
  GAAGCTGGTATTCCTGGGAGACCTGATTCTGCTCCAGGAAAACCAGGGAAGCCAGGATCACCTGGCTTGC
  CTGGAGCACCAGGCCTGCAGGGCCTCCCAGGATCAAGTGTGATATACTGTAGTGTTGGGAACCCCGGACC
  ACAAGGAATAAAAGGCAAAGTTGGTCCCCCAGGAGGAAGAGGCCCAAAAGGAGAAAAAGGAAATGAAGGA
  CTCTGTGCCTGTGAGCCTGGACCCATGGGCCCCCCTGGCCCTCCAGGACTTCCTGGGAGGCAGGGGAGTA
  AGGGAGACTTGGGGCTCCCTGGCTGGCTTGGAACAAAAGGTGACCCAGGACCTCCTGGTGCTGAAGGACC
  TCCAGGGCTACCAGGAAAGCATGGTGCCTCTGGACCACCTGGCAACAAAGGGGCGAAGGGTGACATGGTT
  GTATCAAGAGTTAAAGGGCACAAAGGAGAAAGAGGTCCTGATGGGCCCCCAGGATTTCCAGGGCAGCCAG
  GATCACATGGTCGGGATGGACATGCTGGAGAAAAAGGGGATCCAGGACCTCCAGGGGATCATGAAGATGC
  GACCCCAGGTGGTAAAGGATTTCCTGGACCTCTGGGCCCCCCAGGCAAAGCAGGACCTGTGGGGCCCCCA
  GGACTGGGATTTCCTGGTCCACCAGGAGAGCGAGGCCACCCAGGAGTTCCAGGCCACCCAGGTGTGAGGG
  GCCCTGATGGCTTGAAGGGTCAGAAAGGTGACACAATTTCTTGCAACGTAACCTACCCTGGGAGGCATGG
  CCCTCCAGGTTTTGATGGACCTCCAGGTCCGAAGGGATTTCCAGGTCCCCAAGGTGCCCCTGGGCTGAGT
  GGTTCAGATGGGCATAAAGGCAGACCTGGCACACCAGGAACAGCGGAAATACCAGGTCCACCTGGTTTTC
  GTGGTGACATGGGAGATCCGGGTTTTGGAGGTGAAAAGGGGTCCTCCCCTGTTGGGCCCCCAGGCCCTCC
  CGGCTCACCAGGAGTGAATGGTCAGAAAGGAATCCCGGGAGACCCTGCATTTGGTCACCTGGGACCCCCG
  GGAAAGAGGGGTCTTTCAGGAGTGCCAGGGATAAAAGGACCCAGAGGTGATCCGGGATGTCCAGGGGCTG
  AAGGGCCAGCTGGCATTCCTGGATTCCTAGGTCTCAAAGGTCCCAAAGGCAGAGAGGGACATGCTGGGTT
  TCCAGGTGTCCCAGGTCCACCTGGCCATTCCTGTGAAAGAGGTGCTCCAGGGATACCAGGGCAACCGGGA
  CTCCCTGGGTATCCAGGTAGCCCAGGTGCTCCAGGTGGGAAAGGACAGCCGGGAGATGTGGGGCCTCCCG
  GGCCAGCTGGAATGAAAGGCCTCCCCGGACTCCCAGGACGGCCTGGGGCACATGGTCCCCCAGGCCTCCC
  AGGAATCCCAGGTCCCTTTGGAGATGATGGGCTACCTGGTCCTCCAGGTCCAAAGGGACCCCGGGGGCTG
  CCTGGTTTCCCAGGTTTTCCCGGAGAAAGAGGAAAGCCTGGTGCAGAGGGATGTCCTGGCGCAAAGGGAG
  AACCTGGAGAGAAGGGCATGTCTGGCCTTCCTGGAGACCGGGGACTGAGAGGGGCCAAAGGAGCCATAGG
  ACCTCCCGGAGATGAAGGAGAAATGGCTATCATTTCACAAAAGGGAACACCTGGGGAACCTGGACCTCCT
  GGAGATGATGGATTCCCAGGAGAAAGAGGTGATAAAGGAACTCCCGGGATGCAAGGGAGAAGAGGAGAGC
  CGGGAAGATACGGACCACCTGGATTTCACAGAGGGGAACCTGGTGAGAAAGGTCAGCCAGGGCCTCCTGG
  ACCCCCAGGCCCTCCAGGCTCAACTGGTCTAAGAGGGTTCATTGGTTTTCCAGGACTTCCAGGTGACCAG
  GGTGAGCCAGGTTCTCCAGGTCCCCCTGGATTTTCAGGAATTGATGGAGCAAGAGGACCTAAAGGAAACA
  AAGGTGACCCTGCCAGTCACTTTGGTCCACCTGGTCCAAAGGGTGAGCCAGGTAGCCCTGGATGTCCAGG
  GCATTTTGGAGCATCCGGAGAGCAGGGCTTGCCTGGTATTCAAGGGCCCAGAGGATCACCTGGAAGGCCA
  GGGCCACCTGGCTCCTCTGGACCACCAGGGTGCCCAGGTGATCACGGGATGCCTGGGCTGAGGGGACAGC
  CAGGAGAAATGGGAGACCCTGGGCCAAGAGGCCTCCAGGGGGATCCAGGGATACCAGGTCCTCCGGGAAT
  AAAAGGTCCCTCCGGATCACCTGGCCTGAACGGCTTGCATGGATTGAAAGGTCAGAAAGGAACTAAAGGT
  GCTTCAGGTTTGCATGATGTGGGGCCACCTGGTCCAGTGGGAATACCTGGGCTAAAAGGGGAGAGAGGAG
  ACCCTGGGAGCCCAGGAATCTCTCCTCCAGGTCCTCGTGGAAAGAAAGGTCCCCCAGGACCCCCAGGGAG
  TTCAGGACCACCTGGTCCTGCAGGTGCCACAGGAAGAGCTCCTAAGGACATTCCTGACCCGGGTCCACCT
  GGAGATCAGGGACCTCCTGGTCCTGATGGCCCAAGAGGAGCACCTGGGCCTCCAGGCCTCCCTGGGAGTG
  TTGACCTTCTGAGAGGGGAGCCAGGTGACTGTGGTCTACCAGGGCCACCAGGTCCCCCTGGCCCACCAGG
  CCCTCCAGGATACAAAGGCTTTCCAGGATGTGATGGAAAAGATGGCCAGAAAGGACCAGTGGGATTCCCG
  GGACCGCAGGGACCACATGGATTTCCTGGGCCACCTGGAGAGAAGGGTTTACCTGGACCTCCAGGGAGAA
  AAGGGCCCACTGGTCTTCCGGGTCCCAGAGGTGAACCGGGGCCACCTGCAGATGTGGATGACTGTCCCCG
  AATCCCAGGCCTTCCTGGGGCGCCAGGCATGAGAGGACCAGAAGGAGCCATGGGGCTCCCTGGAATGAGA
  GGCCCCTCAGGACCAGGGTGCAAAGGAGAGCCTGGGCTGGATGGCAGGAGGGGTGTGGATGGCGTCCCTG
  GGTCTCCTGGGCCTCCCGGACGTAAAGGTGACACAGGAGAAGACGGCTACCCTGGAGGACCAGGGCCTCC
  TGGTCCCATTGGGGATCCTGGGCCCAAAGGGTTTGGCCCTGGATACCTCGGTGGCTTCCTCCTGGTTCTC
  CACAGTCAGACGGACCAGGAGCCCACCTGCCCCCTGGGCATGCCCAGGCTCTGGACTGGGTATAGTCTGT
  TATACCTGGAAGGGCAAGAGAAAGCTCACAATCAAGACCTTGGTCTGGCAGGGTCTTGCCTTCCCGTATT
  TAGCACGCTGCCCTTTGCCTACTGCAACATCCACCAGGTGTGCCACTATGCCCAGAGAAACGACAGATCC
  TACTGGCTGGCCAGCGCTGCGCCCCTCCCCATGATGCCACTCTCTGAAGAGGCGATCCGCCCCTATGTCA
  GCCGCTGTGCGGTATGCGAGGCCCCGGCCCAGGCGGTGGCGGTGCACAGCCAGGACCAGTCCATCCCCCC
  ATGTCCGCAGACCTGGAGGAGCCTCTGGATCGGGTATTCATTCCTGATGCACACAGGAGCTGGGGACCAA
  GGAGGAGGGCAGGCCCTTATGTCACCTGGCAGCTGCCTGGAAGATTTCAGAGCAGCACCATTCCTTGAAT
  GCCAGGGCCGGCAGGGAACTTGCCACTTTTTCGCAAATAAGTATAGCTTCTGGCTCACAACGGTGAAAGC
  AGACTTGCAGTTTTCCTCTGCTCCAGCACCAGACACCTTAAAAGAAAGCCAGGCCCAACGCCAGAAAATC
  AGCCGGTGCCAGGTCTGCGTGAAGTATAGCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000092
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000092.4, NP_000083.3
• RefSeq Size: 10341 bp
• RefSeq ORF: 5073 bp
• Locus ID: 1286
• Cytogenetics: 2q36.3
• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
• Gene Summary: 'This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR. [provided by RefSeq, Jul 2008]'