SIX5 (NM_175875) Human Tagged ORF Clone

CAT#: RC211844

SIX5 (Myc-DDK-tagged)-Human SIX homeobox 5 (SIX5)

Product Images

Western blot validation of overexpression lysate (Cat# LY406194) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211844 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SIX5 protein (Cat# TP311844). The protein was produced from HEK293T cells transfected with SIX5 cDNA clone (Cat# RC211844) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIX5
• Synonyms: BOR2; DMAHP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211844 representing NM_175875
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACCTTGCCTGCGGAGCCGAGCGCGGGGCCGGCGGCTGGGGGGGAGGCGGTGGCGGCGGCGGCGG
  CGACCGAAGAGGAGGAGGAGGAAGCGCGCCAGCTCTTGCAGACTTTGCAGGCGGCCGAGGGTGAGGCGGC
  GGCGGCGGCCGGGGCCGGGGCGGGCGCAGCGGCTGCGGGAGCTGAGGGCCCGGGATCCCCGGGCGTCCCC
  GGGTCGCCCCCCGAGGCCGCTTCCGAACCGCCCACGGGCCTCCGCTTCTCGCCCGAGCAGGTGGCGTGCG
  TCTGCGAGGCGCTGCTCCAGGCGGGCCACGCCGGCCGCTTGAGCCGCTTCCTGGGCGCACTGCCCCCGGC
  CGAGCGCCTACGTGGCAGCGACCCGGTGTTGCGCGCGCGGGCCCTGGTGGCCTTCCAGCGGGGCGAGTAC
  GCCGAGCTCTACCGGCTACTCGAGAGCCGCCCCTTCCCCGCCGCCCACCACGCCTTCCTGCAGGACCTCT
  ACCTGCGCGCGCGCTACCATGAGGCCGAGCGGGCCCGCGGCCGCGCGCTTGGCGCAGTGGACAAGTATCG
  ACTGCGCAAGAAGTTCCCGCTGCCCAAGACCATCTGGGACGGCGAGGAGACAGTCTACTGCTTCAAGGAG
  CGCTCCCGCGCAGCGCTCAAGGCCTGCTACCGCGGCAACCGCTACCCCACGCCGGACGAGAAGCGCCGCC
  TGGCCACACTCACCGGCCTGTCGCTCACGCAGGTCAGCAACTGGTTCAAGAACCGGCGACAGCGCGACCG
  GACCGGGGCCGGAGGCGGCGCGCCCTGCAAGAGCGAGTCTGATGGGAATCCCACGACTGAGGACGAGTCC
  AGCCGAAGTCCTGAGGACCTGGAGAGAGGGGCGGCCCCAGTGTCCGCCGAGGCCGCTGCCCAGGGCTCCA
  TATTCCTGGCAGGGACCGGCCCTCCCGCGCCTTGCCCGGCTTCCTCCTCCATCCTGGTGAACGGGAGCTT
  CCTGGCAGCCAGCGGCTCCCCAGCAGTGCTCCTCAACGGGGGCCCCGTCATCATCAACGGCCTGGCCCTG
  GGCGAGGCCTCCAGCCTGGGCCCGCTGCTGCTCACTGGGGGCGGGGGTGCCCCTCCACCGCAGCCCAGCC
  CTCAGGGGGCCAGCGAGACCAAGACCTCTCTGGTCCTGGACCCTCAGACAGGGGAGGTGCGGCTGGAGGA
  GGCTCAGTCGGAGGCCCCTGAGACCAAAGGGGCCCAGGTGGCTGCTCCGGGACCAGCCCTTGGAGAGGAG
  GTCCTGGGGCCCCTGGCCCAAGTGGTGCCTGGCCCCCCGACGGCTGCCACCTTTCCTCTGCCCCCGGGGC
  CAGTGCCTGCTGTGGCTGCCCCACAAGTGGTACCGCTCTCCCCACCCCCGGGGTATCCCACGGGCCTGAG
  CCCCACCTCCCCACTATTGAACCTGCCCCAGGTAGTACCCACCTCACAGGTGGTGACCCTGCCCCAGGCT
  GTGGGGCCCCTGCAGCTGTTGGCAGCCGGGCCAGGCAGCCCTGTGAAGGTGGCAGCTGCAGCAGGCCCTG
  CCAATGTGCACCTCATCAACTCCGGGGTGGGCGTGACTGCCCTGCAGCTGCCTTCGGCCACTGCCCCAGG
  AAACTTCCTCCTGGCCAACCCTGTGTCTGGCAGCCCCATCGTGACGGGTGTGGCCGTGCAGCAGGGCAAG
  ATCATCCTCACCGCCACCTTCCCCACCAGCATGCTCGTCTCCCAGGTCCTGCCGCCAGCCCCCGGCCTGG
  CCCTGCCACTGAAGCCAGAGACGGCCATCTCCGTGCCTGAGGGAGGCCTCCCGGTGGCCCCCAGCCCTGC
  TCTCCCAGAGGCTCACGCCCTAGGCACCCTTTCTGCACAGCAGCCACCCCCCGCCGCTGCCACCACCTCC
  AGCACCAGCCTGTCCTTCTCCCCTGACTCCCCTGGCCTCCTGCCCAACTTCCCGGCGCCCCCACCAGAGG
  GGCTGATGTTGTCACCCGCGGCCGTGCCTGTCTGGTCAGCAGGGCTGGAACTAAGCGCAGGAACAGAGGG
  GCTGCTGGAAGCGGAAAAGGGGCTGGGGACACAGGCCCCCCACACCGTGCTGAGGCTGCCAGACCCCGAC
  CCTGAGGGGCTGCTCCTGGGGGCCACCGCAGGGGGTGAGGTTGACGAGGGGTTGGAAGCTGAGGCCAAGG
  TTCTGACCCAGCTCCAGTCGGTGCCTGTGGAGGAGCCCTTGGAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211844 representing NM_175875
  Red=Cloning site Green=Tags(s)
  
  MATLPAEPSAGPAAGGEAVAAAAATEEEEEEARQLLQTLQAAEGEAAAAAGAGAGAAAAGAEGPGSPGVP
  GSPPEAASEPPTGLRFSPEQVACVCEALLQAGHAGRLSRFLGALPPAERLRGSDPVLRARALVAFQRGEY
  AELYRLLESRPFPAAHHAFLQDLYLRARYHEAERARGRALGAVDKYRLRKKFPLPKTIWDGEETVYCFKE
  RSRAALKACYRGNRYPTPDEKRRLATLTGLSLTQVSNWFKNRRQRDRTGAGGGAPCKSESDGNPTTEDES
  SRSPEDLERGAAPVSAEAAAQGSIFLAGTGPPAPCPASSSILVNGSFLAASGSPAVLLNGGPVIINGLAL
  GEASSLGPLLLTGGGGAPPPQPSPQGASETKTSLVLDPQTGEVRLEEAQSEAPETKGAQVAAPGPALGEE
  VLGPLAQVVPGPPTAATFPLPPGPVPAVAAPQVVPLSPPPGYPTGLSPTSPLLNLPQVVPTSQVVTLPQA
  VGPLQLLAAGPGSPVKVAAAAGPANVHLINSGVGVTALQLPSATAPGNFLLANPVSGSPIVTGVAVQQGK
  IILTATFPTSMLVSQVLPPAPGLALPLKPETAISVPEGGLPVAPSPALPEAHALGTLSAQQPPPAAATTS
  STSLSFSPDSPGLLPNFPAPPPEGLMLSPAAVPVWSAGLELSAGTEGLLEAEKGLGTQAPHTVLRLPDPD
  PEGLLLGATAGGEVDEGLEAEAKVLTQLQSVPVEEPLEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175875
• ORF Size: 2217 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175875.3, NM_175875.4, NP_787071.2
• RefSeq Size: 3145 bp
• RefSeq ORF: 2220 bp
• Locus ID: 147912
• Protein Families: Transcription Factors
• MW: 74.4 kDa
• Gene Summary: The protein encoded by this gene is a homeodomain-containing transcription factor that appears to function in the regulation of organogenesis. This gene is located downstream of the dystrophia myotonica-protein kinase gene. Mutations in this gene are a cause of branchiootorenal syndrome type 2. [provided by RefSeq, Jul 2009]